Identification of a novel hybrid sterility locus S67 between temperate japonica subgroup and basmati subgroup in Oryza sativa L.

Asian cultivated rice (Oryza sativa L.) is the most important cultivated species in the AA genome species of the genus Oryza. basmati is a special and famous subgroup in Asian cultivated rice, and temperate japonica is one of the most important cultivated subgroup, too.

Improving bridge effect to overcome interspecific hybrid sterility by pyramiding hybrid sterile loci from Oryza glaberrima.

In order to evaluate the genetic effect caused by hybrid sterile loci, NILs with O. glaberrima fragment at six hybrid sterile loci under O. sativa genetic background (single-locus-NILs) were developed; two lines harboring two hybrid sterile loci, one line harboring three hybrid sterile loci were further developed.

A toxin-antidote system contributes to interspecific reproductive isolation in rice.

Breakdown of reproductive isolation facilitates flow of useful trait genes into crop plants from their wild relatives. Hybrid sterility, a major form of reproductive isolation exists between cultivated rice (Oryza sativa) and wild rice (O. meridionalis, Mer).

A natural gene drive system confers reproductive isolation in rice.

Hybrid sterility restricts the utilization of superior heterosis of indica-japonica inter-subspecific hybrids. In this study, we report the identification of RHS12, a major locus controlling male gamete sterility in indica-japonica hybrid rice. We show that RHS12 consists of two genes (iORF3/DUYAO and iORF4/JIEYAO) that confer preferential transmission of the RHS12-i type male gamete into the progeny, thereby forming a natural gene drive.

Three QTL from Oryza meridionalis Could Improve Panicle Architecture in Asian Cultivated Rice.

Rice panicle architecture is directly associated with grain yield and is also the key target in high-yield rice breeding program. In this study, three BCF segregation populations derived from the crosses between two accessions of Oryza meridionalis and a O. sativa spp.

Interspecific Hybridization Is an Important Driving Force for Origin and Diversification of Asian Cultivated Rice L.

As one of the most important crops, Asian cultivated rice has evolved into a complex group including several subgroups adapting various eco-climate-systems around the globe. Here, we pictured a comprehensive view of its original domestication, divergences, and the origin of different subgroups by integrating agriculture, archeology, genetics, nuclear, and cytoplasm genome results. Then, it was highlighted that interspecific hybridization-introgression has played important role in improving the genetic diversity and adaptation of during its evolution process.

Understanding the Nature of Hybrid Sterility and Divergence of Asian Cultivated Rice.

Intraspecific hybrid sterility is a common form of postzygotic reproductive isolation in Asian cultivated rice, which is also the major obstacle to utilize the strong heterosis in the rice breeding program. Here, we review recent progress in classification and hybrid sterility in Asian cultivated rice. A genome-wide analysis of numerous wild relatives of rice and Asian cultivated rice has provided insights into the origin and differentiation of Asian cultivated rice, and divided Asian cultivated rice into five subgroups.

A Genetic Resource for Rice Improvement: Introgression Library of Agronomic Traits for All AA Genome Species.

Rice improvement depends on the availability of genetic variation, and AA genome species are the natural reservoir of favorable alleles that are useful for rice breeding. To systematically evaluate and utilize potentially valuable traits of new QTLs or genes for the Asian cultivated rice improvement from all AA genome species, 6,372 agronomic trait introgression lines (ILs) from BC to BC were screened and raised based on the variations in agronomic traits by crossing 170 accessions of 7 AA genome species and 160 upland rice accessions of as the donor parents, with three elite cultivars of , Dianjingyou 1 (a variety), Yundao 1 (a variety), and RD23 (an variety) as the recurrent parents, respectively. The agronomic traits, such as spreading panicle, erect panicle, dense panicle, lax panicle, awn, prostrate growth, plant height, pericarp color, kernel color, glabrous hull, grain size, 1,000-grain weight, drought resistance and aerobic adaption, and blast resistance, were derived from more than one species.

Effects of WeChat platform-based health management on health and self-management effectiveness of patients with severe chronic heart failure.

Background: Epidemiological studies have found that the prevalence of chronic heart failure in China is 0.9%, the number of people affected is more than 4 million, and the 5-year survival rate is even lower than that of malignant tumors.

Aim: To determine the impact of WeChat platform-based health management on severe chronic heart failure patients' health and self-management efficacy.

Natural alleles of a uridine 5'-diphospho-glucosyltransferase gene responsible for differential endosperm development between upland rice and paddy rice.

Traditional upland rice generally exhibits insufficient grains resulting from abnormal endosperm development compared to paddy rice. However, the underlying molecular mechanism of this trait is poorly understood. Here, we cloned the uridine 5'-diphospho (UDP)-glucosyltransferase gene EDR1 (Endosperm Development in Rice) responsible for differential endosperm development between upland rice and paddy rice by performing quantitative trait loci analysis and map-based cloning.

A hybrid sterile locus leads to the linkage drag of interspecific hybrid progenies.

Interspecific hybridization plays an important role in rice breeding by broadening access to desirable traits such as disease resistance and improving yields. However, interspecific hybridization is often hindered by hybrid sterility, linkage drag, and distorted segregation. To mine for favorable genes from , we cultivated a series of BC introgression lines (ILs) of in the rice variety background (Dianjingyou 1) in which the IL-2769 (BCF) showed longer sterile lemmas, wider grains and spreading panicles compared with its receptor parent, suggesting that linkage drag may have occurred.

New Insights Into the Nature of Interspecific Hybrid Sterility in Rice.

Interspecific and intraspecific hybrid sterility is a typical and common phenomenon of postzygotic reproductive barrier in rice. This is an indicator of speciation involved in the formation of new species or subspecies, and it significantly hampers the utilization of favorable genes from distant parents for rice improvement. The genus includes eight species with the same AA genome and is a model plant for studying the nature of hybrid sterility and its relationship with speciation.

Identification and Fine Mapping of (t), a New Gene Conferring Broad-Spectrum Resistance Against From Steud.

The discovery and deployment of new broad-spectrum resistance () genes from cultivated rice and its wild relatives is a strategy to broaden the genetic basis of modern rice cultivars to combat rice blast disease. possessing many valuable traits for tolerance to biotic and abiotic stresses, is an elite gene pool for improvement of Asian cultivated rice. An introgression line IL106 derived from (Acc.

Identification and Validation of Aerobic Adaptation QTLs in Upland Rice.

The aerobic adaptation of upland rice is considered as the key genetic difference between upland rice and lowland rice. Genetic dissection of the aerobic adaptation is important as the basis for improving drought tolerance and terrestrial adaptation by using the upland rice. We raised BC-BC introgression lines (ILs) in lowland rice Minghui 63 (MH63) background.

Two SNP Mutations Turned off Seed Shattering in Rice.

Seed shattering is an important agronomic trait in rice domestication. In this study, using a near-isogenic line (NIL-) from , we found a hybrid seed shattering phenomenon between the NIL- and its recurrent parent, a variety Yundao 1. The heterozygotes at () exhibited the shattering phenotype, whereas the homozygotes from both parents conferred the non-shattering.

Mapping and breeding value evaluation of a semi-dominant semi-dwarf gene in upland rice.

Plant height is an important trait related to yield potential and plant architecture. A suitable plant height plays a crucial role in improvement of rice yield and lodging resistance. In this study, we found that the traditional upland landrace 'Kaowenghan' (KWH) showed a special semi-dwarf phenotype.

Mapping five novel interspecific hybrid sterility loci between and .

is a potential source for improving Asian cultivated rice via direct hybridization and backcrossing. However, hybrid sterility between and is the main barrier of reproduction hindering the transfer of favorable genes from to . To investigate the nature of hybrid sterility between and , three accessions of were used as male parents to cross Dianjingyou 1, an subsp.

Neutral alleles at hybrid sterility loci of from AA genome relatives in Genus .

Hybrid sterility between and is a main reproduction barrier when transferring the favorable alleles from to and it happens due to allelic interaction at sterility loci. Neutral alleles at each locus have the potential to overcome the sterility between the two cultivated rice species. In this study, an cultivar Dianjingyou 1 (DJY1) and its near-isogenic lines (NILs) harboring the single sterility allele -glab, -glab, -glab, -glab, -glab and -glab as the tested lines were crossed with , , , , , and so as to detect the neutral alleles of these loci.

A selfish genetic element confers non-Mendelian inheritance in rice.

Selfish genetic elements are pervasive in eukaryote genomes, but their role remains controversial. We show that , a major quantitative genetic locus for hybrid male sterility between wild rice () and Asian cultivated rice (), contains two tightly linked genes [ () and ]. encodes a toxic genetic element that aborts pollen in a sporophytic manner, whereas encodes an antidote that protects pollen in a gametophytic manner.

Mapping of (t) responsible for interspecific hybrid sterility between and .

originates from South America continent and contains many valuable traits, such as tolerance to abiotic stress, high yield and good cooking qualities. However, hybrid sterility severely hindered the utilization of favorable genes of by interspecific hybridization. In order to further understand the nature of hybrid sterility between and , a near isogenic line (NIL) was developed using a variety Dianjingyou 1 as the recurrent parent and an accession of as the donor parent.

G-protein βγ subunits determine grain size through interaction with MADS-domain transcription factors in rice.

The simultaneous improvement of grain quality and yield of cereal crops is a major challenge for modern agriculture. Here we show that a rice grain yield quantitative trait locus qLGY3 encodes a MADS-domain transcription factor OsMADS1, which acts as a key downstream effector of G-protein βγ dimers. The presence of an alternatively spliced protein OsMADS1 is shown to be associated with formation of long and slender grains, resulting in increases in both grain quality and yield potential of rice.

Fine mapping of Pi57(t) conferring broad spectrum resistance against Magnaporthe oryzae in introgression line IL-E1454 derived from Oryza longistaminata.

Wild species of the genus Oryza are excellent gene pools for improvement of agronomic traits of Asian cultivated rice. The blast resistance gene Pi57(t) in the introgression line IL-E1454 derived from Oryza longistaminata was previously mapped on rice chromosome 12. Inoculation with 322 Magnaporthe oryzae isolates collected from 6 countries indicated that Pi57(t) conferred broad spectrum resistance against M.

Mapping QTLs for hybrid sterility in three AA genome wild species of Oryza.

In order to know the genetic nature of hybrid sterility further, three populations, a BC4F2 population derived from Oryza nivara crossed with Yundao 1, a BC4F2 population derived from O. rufipogon crossed with Yundao 1, and a BC10F1 population derived from a cross between O. barthii and Dianjingyou 1 were developed, respectively.

Fine mapping of S37, a locus responsible for pollen and embryo sac sterility in hybrids between Oryza sativa L. and O. glaberrima Steud.

Hybrid sterility locus S37 between Oryza glaberrima and Oryza sativa results in both pollen and embryo sac sterility. Interspecific crossing between African cultivated rice Oryza glaberrima and Oryza sativa cultivars is hindered by hybrid sterility. To dissect the mechanism of interspecific hybrid sterility, we developed a near-isogenic line (NIL)-S37 using Dianjingyou1 (DJY1) as the recipient parent and an African cultivated rice variety as the donor parent.