Application and evaluation of virtual simulation technology in 'corneal contact lens' education.

Background: To evaluate the impact of the corneal contact lens virtual simulation teaching system on students' course experience and self-directed learning in 'corneal contact lens' education.

Methods: This study developed a virtual simulation experimental teaching system for 'corneal contact lens' education. A longitudinal comparison of the course experience and self-directed learning abilities of the same group of students at a university in Fujian Province before and after using the system was conducted through questionnaires.

A relative-independent haplotype derivation method applied for noninvasive prenatal testing for chromosomal rearrangements in a pregnant carrier.

This study aimed to perform noninvasive prenatal testing for structural chromosomal rearrangements (NIPT-SR) for a female pregnant proband carrying a t(4;8) balanced translocation, whose husband exhibited a normal karyotype. NIPT-SR could accurately detect transmission status of structural rearrangements in fetus through Hidden Markov Model (HMM) analysis, which requires the construction of parental haplotypes. To address the challenge of lacking genetic information from other family members of this proband, we developed a novel strategy to infer the fetal inheritance of structural variants by integrating Oxford Nanopore Technologies (ONT) with the NIPT-SR approach.

Meibomian gland alterations in allergic conjunctivitis: insights from a novel quantitative analysis algorithm.

Purpose: To investigate the changes in meibomian gland (MG) structure in allergic conjunctivitis (AC) patients using an intelligent quantitative analysis algorithm and to explore the relationship between these changes and clinical parameters.

Methods: A total of 252 eyes from patients with AC and 200 eyes from normal controls were examined. Infrared meibography was performed using the non-contact mode of the Keratograph 5M.

Fluid Classification via the Dual Functionality of Moisture-Enabled Electricity Generation Enhanced by Deep Learning.

Classifications of fluids using miniaturized sensors are of substantial importance for various fields of application. Modified with functional nanomaterials, a moisture-enabled electricity generation (MEG) device can execute a dual-purpose operation as both a self-powered framework and a fluid detection platform. In this study, a novel intelligent self-sustained sensing approach was implemented by integrating MEG with deep learning in microfluidics.

An integrative predictive model for orthokeratology lens decentration based on diverse metrics.

Purpose: To develop a predictive model for orthokeratology (Ortho-K) lens decentration 1 month after wear.

Methods: This study included myopic children who were fitted with Ortho-K lenses at Fujian Provincial Hospital between December 2022 and May 2024. Corneal topography parameters and other relevant metrics were collected pre- and post-treatment.

Efficacy analysis of HAIC combined with lenvatinib plus PD1 inhibitor vs. first-line systemic chemotherapy for advanced intrahepatic cholangiocarcinoma.

This research was intended to compare the clinical efficacy of hepatic arterial infusion chemotherapy (HAIC) in conjunction with lenvatinib and PD1 inhibitors to first-line systemic chemotherapy for advanced intrahepatic cholangiocarcinoma(ICC). The research enrolled advanced ICC patients who underwent HAIC plus lenvatinib and PD1 inhibitor(n = 51) or first-line systemic chemotherapy(cisplatin + gemcitabine, n = 39) between July 2020 to January 2023 in Zhongshan People's Hospital.Their clinical outcomes were assessed through measurement of parameters encompassing objective response rate (ORR), disease control rate (DCR), median overall survival (mOS), median progression-free survival (mPFS), median duration of response (mDOR), and treatment-related adverse events (TRAEs).

Elevated Risk of Adverse Prognosis in Patients with T2-3 Stage Breast Cancer Exhibiting Non-Pathological Complete Response Following Neoadjuvant Chemotherapy: Significance of Regenerating Islet-Derived Family Member 4.

Objective: In this study, we aimed to establish the role of regenerating islet-derived family member 4 (Reg IV) as an independent risk factor and prognostic predictor in patients with T2-3 stage breast cancer who exhibit a non-pathological complete response (non-pCR) following neoadjuvant chemotherapy (NACT). Additionally, we examined the potential correlation and interaction between Reg IV and epidermal growth factor receptor (EGFR).

Methods: A total of 67 patients with T2-3 stage breast cancer exhibiting non-pCR after NACT between September 2019 and December 2021 were included in this study.

Optimization of preparation conditions for β-chitosan derived from diatom biomanufacturing using response surface methodology.

Chitosan is a polymeric polysaccharide with widely application. At present, commercialized chitosan obtained by deacetylating chitin with acid-alkali method. The homogeneity of the molecular weight of chitosan is difficult to adjust due to the low homogeneity of chitosan itself and the degradation effect of the extraction process.

An arteriovenous fistula model: Carotid artery-posterior facial vein fistula in rats.

Objective: To describe an alternative arteriovenous fistula (AVF) model involving anastomosis of the common carotid artery (CCA) with the posterior facial vein (PFV).

Methods: Twenty-two male Sprague-Dawley rats (age 6-8 weeks) were used to establish the AVF model involving end-to-side anastomosis of PFV and CCA. The peak velocity of the CCA and the diameter of the outflow vein were recorded at 7, 14, and 42 days after the operation using Doppler ultrasound.

Development and validation of a neoadjuvant chemotherapy pathological complete remission model based on Reg IV expression in breast cancer tissues: a clinical retrospective study.

Objective: To develop and authenticate a neoadjuvant chemotherapy (NACT) pathological complete remission (pCR) model based on the expression of Reg IV within breast cancer tissues with the objective to provide clinical guidance for precise interventions.

Method: Data relating to 104 patients undergoing NACT were collected. Variables derived from clinical information and pathological characteristics of patients were screened through logistic regression, random forest, and Xgboost methods to formulate predictive models.

Theoretical Study of Dissolved Gas Molecules in Transformer Oil Adsorbed on Intrinsic and TM (Ta, V)-Doped MoTe Monolayer.

In this paper, CH, CH, H, and CO adsorbed on intrinsic MoTe monolayer and transition metal atom (Ta, V)-doped MoTe monolayer have been investigated with density functional theory based on first-principles study. The adsorption energy, geometries, band structures, and density of states of four gases (CH, CH, H, and CO) adsorbed on the MoTe and doped MoTe surfaces were analyzed. The results shown that the gas adsorption performance of transition metal atom (Ta, V)-doped MoTe monolayers is more superior than that of intrinsic MoTe, and the adsorption energy and charge transfer of the adsorbed gases on the TM-MoTe monolayer are significantly increased in comparison with both sides.

The role of ferroptosis in DM-induced liver injury.

The liver damage caused by Diabetes Mellitus (DM) has attracted increasing attention in recent years. Liver injury in DM can be caused by ferroptosis, a form of cell death caused by iron overload. However, the role of iron transporters in this context is still not clear.

Meibomian glands segmentation in infrared images with limited annotation.

Aim: To investigate a pioneering framework for the segmentation of meibomian glands (MGs), using limited annotations to reduce the workload on ophthalmologists and enhance the efficiency of clinical diagnosis.

Methods: Totally 203 infrared meibomian images from 138 patients with dry eye disease, accompanied by corresponding annotations, were gathered for the study. A rectified scribble-supervised gland segmentation (RSSGS) model, incorporating temporal ensemble prediction, uncertainty estimation, and a transformation equivariance constraint, was introduced to address constraints imposed by limited supervision information inherent in scribble annotations.

Uncovering specific taxonomic and functional alteration of gut microbiota in chronic kidney disease through 16S rRNA data.

Introduction: Chronic kidney disease (CKD) is worldwide healthcare burden with growing incidence and death rate. Emerging evidence demonstrated the compositional and functional differences of gut microbiota in patients with CKD. As such, gut microbial features can be developed as diagnostic biomarkers and potential therapeutic target for CKD.

AI-enhanced biomedical micro/nanorobots in microfluidics.

Human beings encompass sophisticated microcirculation and microenvironments, incorporating a broad spectrum of microfluidic systems that adopt fundamental roles in orchestrating physiological mechanisms. recapitulation of human microenvironments based on lab-on-a-chip technology represents a critical paradigm to better understand the intricate mechanisms. Moreover, the advent of micro/nanorobotics provides brand new perspectives and dynamic tools for elucidating the complex process in microfluidics.

A fNIRS investigation of menopausal-related symptoms and brain cortical activity in menopause.

Background: This study aimed to delineate the association between menopausal-related symptoms and brain cortical hemodynamics in peri-postmenopause women.

Methods: Cross-sectional data from a total of 358 Han-Chinese women who visited the Menopause Clinic in the Shanghai Sixth People's Hospital from August 2019 to August 2022. Menopausal-related symptoms were analyzed through Kupperman index (KMI) scale and PSQI scale, while cerebral blood flow was measured using a functional near-infrared spectroscopy (fNIRS).

Effects of orthokeratology lenses on tear film and tarsal glands and control of unilateral myopia in children.

To investigate the effects of an orthokeratology lens on the tear film and tarsal glands and myopia control in children with unilateral myopia using an intelligent analysis model. We retrospectively reviewed the medical records from November 2020 to November 2022 of 68 pediatric patients with unilateral myopia in Fujian Provincial Hospital who had been wearing an orthokeratology lens for more than 1 year. The 68 myopic eyes were included in the treatment group, while the 68 healthy, untreated contralateral eyes were included in the control group.

Inhibition of Fatty Acid β-Oxidation by Fatty Acid Binding Protein 4 Induces Ferroptosis in HK2 Cells Under High Glucose Conditions.

Backgruound: Ferroptosis, which is caused by an iron-dependent accumulation of lipid hydroperoxides, is a type of cell death linked to diabetic kidney disease (DKD). Previous research has shown that fatty acid binding protein 4 (FABP4) is involved in the regulation of ferroptosis in diabetic retinopathy. The present study was constructed to explore the role of FABP4 in the regulation of ferroptosis in DKD.

Diatomite hemostatic particles with hierarchical porous structure for rapid and effective hemostasis.

The development of fast, safe and effective hemostatic materials is crucial for pre-hospital first aid. In this study, diatomite hemostatic granules (Dhp) were developed by rotating granulation method using silica sol as binder. During rotating granulation process, the Pre-Dhp were prepared by rolling snowball effect, in which nano-silica in silica sol uniformly distributed on the surface of diatomite and polymerized through hydrogen bond to produce strong adhesion.

Sinigrin Impedes the Breast Cancer Cell Growth through the Inhibition of PI3K/AKT/mTOR Phosphorylation-Mediated Cell Cycle Arrest.

Breast carcinoma, one of the most lethal variants of carcinogenesis, significantly diagnosed type of cancer amongst the female population. Sinigrin, also known as glucosinolate, is found in the seeds of Brassica nigra and shown to enhance various cancer cells potentially. Nevertheless, the mechanistic explanation of sinigrin (SGN)-mediated breast cancer growth and augmentation is still to be investigated.

Rapid detection of the New Delhi metallo-β-lactamase (NDM) gene by recombinase polymerase amplification.

New Delhi metallo-β-lactamase (NDM) is a series of enzyme conferring resistance to β-lactam antibiotics including the carbapenems. The bla gene has been reported in a variety of Gram-negative bacilli, especially in the Enterobacteriaceae and Acinetobacter spp., which is deeply disconcerting for public health worldwide.

[A novel method of optic disk segmentation based on visual saliency and rotary scanning].

Fast optic disk localization and boundary segmentation is an important research topic in computer aided diagnosis. This paper proposes a novel method to effectively segment optic disk by using human visual characteristics in analyzing and processing fundus image. After a general analysis of optic disk features in fundus images, the target of interest could be located quickly, and intensity, color and spatial distribution of the disc are used to generate saliency map based on pixel distance.