Research of Fluridone's Effects on Growth and Pigment Accumulation of Based on Transcriptome Sequencing.

has high economic value because of its high astaxanthin-producing ability. The mutation breeding of is an important method to improve the yield of astaxanthin. Fluoridone, an inhibitor of phytoene dehydrogenase, can be used as a screening reagent for mutation breeding of .

Characterization of deoxyribonucleic methylation and transcript abundance of sex-related genes during tempera ture-dependent sex determination in Mauremys reevesii†.

A number of genes relevant for sex determination have been found in species with temperature-dependent sex determination. Epigenetics play a key role in sex determination, but characterization of deoxyribonucleic acid methylation of sex-related genes on temperature-dependent sex determination remains unclear. Mauremys reevesii is a typical species with temperature-dependent sex determination.

[miR-593 inhibits proliferation of colon cancer cells by down-regulating PLK1].

Objective: To explore the role of miR-593 in regulating the proliferation of colon cancer cells and the molecular mechanism.

Methods: Bioinformatics analysis identified PLK1 as the possible target gene of miR-593. Luciferase assay was employed to verify the binding between miR-593 and PLK1, and qRT-PCR and Western blotting were used to verify that PLK1 was the direct target gene of miR-593.

Transcriptome sequencing and comparative analysis of adult ovary and testis identify potential gonadal maintenance-related genes in with temperature-dependent sex determination.

is a classical organism with temperature-dependent sex determination (TSD). Gonad development in early life has recently received considerable attention but gonadal maintenance after sex differentiation in turtles with TSD remains a mystery. In this study, we sequenced the transcriptomes for the adult testis and ovary using RNA-seq, and 36,221 transcripts were identified.

Detecting coevolution of positively selected in turtles sperm-egg fusion proteins.

Physically interacting sperm-egg proteins have been identified using gene-modified animals in some mammal species. Three proteins are essential for sperm-egg binding: Izumo1 on the sperm surface, and JUNO and CD9 on the egg surface. Most proteins linked to reproductive function evolve rapidly among species by positive selection, and have correlated evolutionary rates to compensate for changes on both the sperm and egg.

miR-21-5p induces cell proliferation by targeting TGFBI in non-small cell lung cancer cells.

The mortality rate of non-small cell lung cancer (NSCLC) remains high worldwide. miR-21-5p plays an important part in many cancer types, including NSCLC. However, the effect of miR-21-5p in NSCLC tumorigenesis remains poorly understood.

Single-molecule long-read transcriptome profiling of Platysternon megacephalum mitochondrial genome with gene rearrangement and control region duplication.

Platysternon megacephalum is the sole living representative of the poorly studied turtle lineage Platysternidae. Their mitochondrial genome has been subject to gene rearrangement and control region duplication, resulting in a unique mitochondrial gene order in vertebrates. In this study, we sequenced the first full-length turtle (P.

miR-24-3p promotes cell migration and proliferation in lung cancer by targeting SOX7.

Lung cancer (LC) is one of the leading causes of cancer-related death in the world. miR-24-3p plays critical roles in many cancer types, including LC. In this study, we first investigated whether miR-24-3p promoted LC cell migration and proliferation in vitro.

Complete mitochondrial genome of the (Chelonia: Geoemydidae).

In this study, we obtained complete mitochondrial genome sequence of . The mitochondrial genome reaches a length of 16,527 bp, containing 13 protein-coding genes (PCGs), 22tRNA genes, 2 rRNA genes and 1 control region. All protein-coding genes initiate with ATG as start codon, except for started with GTG.