Phylogeny-Informed Discovery of New Glycopeptide Antibiotics Produced by a Minimal Biosynthetic Gene Cluster.

Glycopeptide antibiotics (GPAs) are the frontline medicines to treat multidrug-resistant Gram-positive infections. Resistance to GPAs warrants the development of new antibiotics. Phylogeny reclassification of GPA biosynthetic gene clusters with a focus on the type V subgroup bearing a heptapeptide scaffold identified new GPAs, varsomycins, possessing unprecedented 3,5-dihydroxyphenylalanine at position 5 and an F-G biphenyl cross-link.

Efficient spatial self-phase modulation in the near-infrared and visible regimes of transition metal carbonitride TiCN.

Transition metal carbides, nitrides, and carbonitrides (MXenes) have attracted great interest in all-optical modulation applications due to their excellent nonlinear optical properties. Herein, we investigated the spatial self-phase modulation (SSPM) of TiCN flake suspension at wavelengths ranging from 405 to 1064 nm. Broadband third-order nonlinear optical susceptibility values of TiCN were found to be ∼10 e.

An Efficient MEMS Microelectrode Array with Reliable Interelectrode Insulation Processes for In Vivo Neural Recording.

Microelectrode arrays, particularly Utah arrays, offer irreplaceable advantages in clinical applications and play a crucial role in advancing brain-computer interactions. However, the glass-fused monolithic structure of Utah arrays limits functional expansion, and the glass insulation process is complex, costly, and time-intensive. This paper presents a microelectrode array with a simple and time-saving fabrication process, utilizing low-resistance silicon and borosilicate glass wafers as electrodes and insulation substrates, respectively.

Laser-induced hole coherence in 2D antiferromagnet MPS through spatial self-phase modulation.

Transition metal phosphorus sulfides (MPS), a family of two-dimensional magnetic materials with a van der Waals structure, exhibit promising applications in nonlinear optical devices. The emergence of carrier coherence in MPS is a fascinating topic in coherently controlling the nonlinear effect (or other novel phenomena). Herein, we systematically investigated the third-order nonlinear optical responses of MPS (M = Ni, Fe, Mn) flake suspensions based on spatial self-phase modulation (SSPM) effect.

4D-Printed Magnetic Responsive Bilayer Hydrogel.

Despite its widespread application in targeted drug delivery, soft robotics, and smart screens, magnetic hydrogel still faces challenges from lagging mechanical performance to sluggish response times. In this paper, a methodology of in situ generation of magnetic hydrogel based on 3D printing of poly-N-isopropylacrylamide (PNIPAM) is presented. A temperature-responsive PNIPAM hydrogel was prepared by 3D printing, and FeO magnetic particles were generated in situ within the PNIPAM network to generate the magnetic hydrogel.

Controllable tip exposure of ultramicroelectrodes coated by diamond-like carbon via direct microplasma jet for enhanced stability and fidelity in single-cell recording.

Precise and long-term electroanalysis at the single-cell level is crucial for the accurate diagnosis and monitoring of brain diseases. The reliable protection in areas outside the signal acquisition points at sharp ultramicroelectrode (UME) tips has a significant impact on the sensitivity, fidelity, and stability of intracellular neural signal recording. However, it is difficult for existing UMEs to achieve controllable exposure of the tip functional structure, which affects their ability to resist environmental interference and shield noise, resulting in unsatisfactory signal-to-noise ratio and signal fidelity of intracellular recordings.

Targeting estrogen-regulated system x promotes ferroptosis and endocrine sensitivity of ER+ breast cancer.

Estrogen receptor positive (ER+) breast cancer accounts for approximately 70% of cases. Endocrine therapies targeting estrogen are the first line therapies for ER+ breast cancer. However, resistance to these therapies occurs in about half of patients, leading to decreased survival rates.

USP35 promotes the growth of ER positive breast cancer by inhibiting ferroptosis via BRD4-SLC7A11 axis.

Anti-estrogen endocrine therapies greatly improve survival of estrogen receptor positive (ER + ) breast cancer. Unfortunately, about 30% of patients do not respond to endocrine therapies initially. We previously showed that deubiquitinase USP35 and ERα act in a positive feedback loop to promote the carcinogenesis of ER+ breast cancer although it is unclear whether USP35 regulates cell death in ER+ breast cancer.

A Wavy Multiple Tm(III)-Containing Open Wells-Dawson Silicotungstate: Synthesis, Structure, and Catalytic Application.

A Tm(III)-rich silicotungstate Na{Tm(HO)[Tm(HO)(SiWO)]}·35HO () based on the open Wells-Dawson-type [SiWO] building unit was synthesized by the reaction of Na[α-SiWO]·18HO, itaconic acid, and TmCl in a HAc/NaAc buffer solution. Five kinds of Tm(III) ions were found in this compound and further linked the {TmSiW} subunit to form an interesting wavy 1D chain structure, which achieved the introduction of more lanthanide (Ln) ions into the [SiWO] unit for the first time. contains multiple exposed Tm-metal active sites, making it an efficient catalyst for the acetalization of 2-aminobenzamides/2-aminobenzenesulphonamides with aldehydes.

An Opto-electrophysiology Neural Probe with Photoelectric Artifact-Free for Advanced Single-Neuron Analysis.

Opto-electrophysiology neural probes targeting single-cell levels offer an important avenue for elucidating the intrinsic mechanisms of the nervous system using different physical quantities, representing a significant future direction for brain-computer interface (BCI) devices. However, the highly integrated structure poses significant challenges to fabrication processes and the presence of photoelectric artifacts complicates the extraction and analysis of target signals. Here, we propose a highly miniaturized and integrated opto-electrophysiology neural probe for electrical recording and optical stimulation at the single-cell/subcellular level.

Flexible Sono-Piezo Patch for Functional Sweat Gland Repair through Endogenous Microenvironmental Remodeling.

Remodeling the endogenous regenerative microenvironment in wounds is crucial for achieving scarless, functional tissue regeneration, especially the functional recovery of skin appendages such as sweat glands in burn patients. However, current approaches mostly rely on the use of exogenous materials or chemicals to stimulate cell proliferation and migration, while the remodeling of a pro-regenerative microenvironment remains challenging. Herein, we developed a flexible sono-piezo patch (fSPP) that aims to create an endogenous regenerative microenvironment to promote the repair of sweat glands in burn wounds.

Beyond the Focus on Individuals: Adding Environment Into the Redefined Successful Aging Paradigm.

Since the origin of the successful aging (SA) model by Rowe and Kahn, scholars have been working on enriching the content of SA and taking actions to promote this concept worldwide. While most studies primarily focus on older individuals, only a few scholars have considered the environmental aspect of SA. However, the environment, directly and indirectly, enhances older adults' abilities to achieve SA.

SLC44A2 regulates vascular smooth muscle cell phenotypic switching and aortic aneurysm.

Aortic aneurysm is a life-threatening disease with limited interventions that is closely related to vascular smooth muscle cell (VSMC) phenotypic switching. SLC44A2, a member of the solute carrier series 44 (SLC44) family, remains undercharacterized in the context of cardiovascular diseases. Venn diagram analysis based on microarray and single-cell RNA sequencing identified SLC44A2 as a major regulator of VSMC phenotypic switching in aortic aneurysm.

Triptolide decreases podocytes permeability by regulating TET2-mediated hydroxymethylation of ZO-1.

Podocyte injury or dysfunction can lead to proteinuria and glomerulosclerosis. Zonula occludens 1 (ZO-1) is a tight junction protein which connects slit diaphragm (SD) proteins to the actin cytoskeleton. Previous studies have shown that the expression of ZO-1 is decreased in chronic kidney disease (CKD).

Improving product quality and productivity of an antibody-based biotherapeutic using inverted frustoconical shaking bioreactors.

The Chinese hamster ovarian (CHO) cells serve as a common choice in biopharmaceutical production, traditionally cultivated in stirred tank bioreactors (STRs). Nevertheless, the pursuit of improved protein quality and production output for commercial purposes demand exploration into new bioreactor types. In this context, inverted frustoconical shaking bioreactors (IFSB) present unique physical properties distinct from STRs.

In situ self-referenced intracellular two-electrode system for enhanced accuracy in single-cell analysis.

Since the emergence of single-cell electroanalysis, the two-electrode system has become the predominant electrochemical system for real-time behavioral analysis of single-cell and multicellular populations. However, due to the transmembrane placement of the two electrodes, cellular activities can be interrupted by the transmembrane potentials, and the test results are susceptible to influences from factors such as intracellular solution, membrane, and bulk solution. These limitations impede the advancement of single-cell analysis.

KCTD5 regulates Ikaros degradation induced by chemotherapeutic drug etoposide in hematological cells.

Therapy-related leukemia carries a poor prognosis, and leukemia after chemotherapy is a growing risk in clinic, whose mechanism is still not well understood. Ikaros transcription factor is an important regulator in hematopoietic cells development and differentiation. In the absence of Ikaros, lymphoid cell differentiation is blocked at an extremely early stage, and myeloid cell differentiation is also significantly affected.

A novel IQ mismatch compensation method in wideband direct-conversion receivers.

Direct-conversion receivers (DCRs) have been widely used in recent years due to their small size and low power consumption. However, the mismatch between the in-phase (I) and the quadrature (Q) branches will seriously affect the performance of the DCRs. This paper proposes a novel blind compensation method to suppress the interference introduced by IQ mismatch.

Do People Prefer Home Palliative Care? A Survey Study and Assessment of Associated Factors in China.

This study examined people's preference for the location to receive palliative care services and determined the associated factors. A questionnaire with reference to the Chinese version of the Hospice Attitude Scale and the Death Correspondence Scale was designed, piloted, revised, and distributed online and in person to collect data (N = 762). Binary logistic regression was used to analyze the effects of relevant factors.

Dissecting climate change risk and financial market instability: Implications for ecological risk management.

This research investigates the impact of climate challenges on financial markets by introducing an innovative approach to measure climate risk, specifically the aggregate climate change concern (ACCC) index. The study aims to assess and quantify the potential influence of climate change and risk-related factors on the performance and dynamics of financial markets. In this paper, concern is defined as the attention paid to the risk of climate change and the associated negative consequences.

PRRG4 regulates mitochondrial function and promotes migratory behaviors of breast cancer cells through the Src-STAT3-POLG axis.

Background: Breast cancer is the leading cause of cancer death for women worldwide. Most of the breast cancer death are due to disease recurrence and metastasis. Increasingly accumulating evidence indicates that mitochondria play key roles in cancer progression and metastasis.

Multifunctional IrOx Neural Probe for In Situ Dynamic Brain Hypoxia Evaluation.

Perioperative cerebral hypoxia and neonatal hypoxia-ischemic encephalopathy are the main triggers that lead to temporary or permanent brain dysfunction. The pathogenesis is intimately correlated to neural activities and the pH of the microenvironment, which calls for a high demand for in situ multitype physiological signal acquisition in the brain. However, conventional pH sensing neural interfaces cannot obtain the characteristics of multimodes, multichannels, and high spatial resolution of physiological signals simultaneously.