Novel electrochemiluminescence platform utilizing AuNPs@Uio-66-NH bridged luminescent substrates and aptamers for the detection of pesticide residues in Chinese herbal medicines.

A large number of Chinese herbal medicines (CHMs) are included in daily recipes, but their pesticide residues have aroused more and more concerns. In this paper, an electrochemiluminescence aptasensor was constructed for the trace detection of acetamiprid (ACE) in Angelica sinensis and Lycium barbarum. Possessing a large specific surface area, UiO-66 was modified with amino groups to improve biocompatibility, and the addition of AuNPs allowed UiO-66-NH to catalyze the formation of excited states of luminescent molecules (TPrA; Ru(bpy)), and AuNPs@UiO-66-NH was used to bridge the aptamer (Au-S) and luminescent substrate (peptide bond).

Novel electrochemiluminescence sensing platform for ultrasensitive detection of malathion residue in tea based on SiONSs doped Luminol/AgNPs as a signal amplification strategy.

To address the potential hazards of organophosphorus pesticides (OPs) residues in tea, an electrochemiluminescence (ECL) aptasensor based on functionalized nanomaterials was constructed in this work. Firstly, gold nanoparticles (AuNPs) were attached on the surface of multi-walled carbon nanotubes (MWCNTs) by the constant potential electrodeposition to form a compound, and it was utilized to provide excellent immobilization sites for complementary DNA (cDNA). Subsequently, composite nanomaterials were synthesized by a one-pot method with aminated Luminol/silver nanoparticles@silica nanospheres (NH-Luminol/Ag@SiONSs).

Preparation of dual recognition adsorbents based on molecularly imprinted polymers and aptamer for highly sensitive recognition and enrichment of ochratoxin A.

In light of the significant risks that mycotoxins posed to public health and environmental safety, this research developed an adsorbent MIPs/Apt/AuNPs@ZIF-67 (MA-AZ) utilizing a dual-recognition approach combining molecularly imprinted polymers (MIPs) and aptamer (Apt). This innovative method enabled the effective and highly selective recognition and enrichment of ochratoxin A (OTA). ZIF-67 was utilized as a carrier with a substantial specific surface area, and gold nanoparticles (AuNPs) were loaded on its surface to fix the thiol-modified Apt on the surface of the carrier.

Recent progress of the research of metal-organic frameworks-molecularly imprinted polymers (MOFs-MIPs) in food safety detection field.

Food safety is an important cornerstone of protecting human health and life. Therefore, it is of great significance to detect possible pollutants in food sensitively and efficiently. Molecularly imprinted polymers (MIPs) and metal-organic frameworks (MOFs) have been widely used in the adsorption and detection of food pollutants.

Novel cross-linkable fluorescent probe with oriented antibody to enhance lateral immunoassay strip for the detection of acetamiprid.

Time-resolved fluorescent lateral immunoassay strip (TRFLIS) is a reliable and rapid method for detecting acetamiprid. However, its sensitivity is often affected by the structural patterns and stability of the fluorescent probe. Researchers have shown significant interests in using goat anti-mouse IgG (GaMIgG) which is indirectly bound to time-resolved fluorescent microsphere (TRFM) and antibody.

Metal-organic framework-based aptasensor utilizing a novel electrochemiluminescence system for detecting acetamiprid residues in vegetables.

The work was based on N-(4-Aminobutyl)-N-ethylisoluminol (ABEI)-functionalized Fe-MIL-101 and gold nanoparticles (AuNPs) as sensing materials, and an electrochemiluminescence (ECL) aptasensor was constructed for detecting acetamiprid. As a metal-organic framework (MOF) material, Fe-MIL-101, was renowned for its unique three-dimensional network structure and efficient catalytic capability. ABEI, a common ECL reagent, was widely applied.

A novel self-enhanced electrochemiluminescent aptamer sensor based on ternary nanocomposite PEI/RuSi-MWCNTs for the detection of profenofos residues in vegetables.

In this work, a novel ternary nanocomposite of PEI/RuSi-MWCNTs was designed and synthesized for the first time, which an ultrasensitive and self-enhanced electrochemiluminescent (ECL) aptasensor was developed for the detection of profenofos residues in vegetables. The self-enhanced complex PEI-Ru (II) enhanced the emission and stability of ECL, and the multi-walled carbon nanotubes (MWCNTs) acted as an excellent carrier and signal amplification. The PEI/RuSi-MWCNTs were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive spectrometer (EDS).

Electrochemiluminescence Sensor Based on CTS-MoS and AB@CTS with Functionalized Luminol for Detection of Malathion Pesticide Residues.

The accumulation of pesticide residues poses a significant threat to the health of people and the surrounding ecological systems. However, traditional methods are not only costly but require expertise in analysis. An electrochemiluminescence (ECL) aptasensor was developed using chitosan and molybdenum disulfide (CTS-MoS), along with acetylene black (AB@CTS) for the rapid detection of malathion residues.

Advances in signal amplification strategies applied in pathogenic bacteria apta-sensing analysis-A review.

Pathogenic bacteria are primarily kinds of food hazards that provoke serious harm to human health via contaminated or spoiled food. Given that pathogenic bacteria continue to reproduce and expand once they contaminate food, pathogenic bacteria of high concentration triggers more serious losses and detriments. Hence, it is essential to detect low-dose pollution at an early stage with high sensitivity.

Novel electrochemiluminescence aptasensor based on AuNPs-ABEI encapsulated TiO nanorod for the detection of acetamiprid residues in vegetables.

Gold nanoparticles (AuNPs)@N-(4-aminobutyl)-N-ethylisoluminol (ABEI)@Titanium dioxide nanorods (TiONRs) were used as sensing materials to produce a unique encapsulated nanostructure aptasensor for the detection of acetamiprid residues in this work. ABEI, an analog of luminol, was extensively used as an electrochemiluminescence (ECL) reagent. The ECL mechanism of ABEI- hydrogen peroxide (HO) system had connections to a number of oxygen-centered free radicals.

Molecularly imprinted polymers-aptamer electrochemical sensor based on dual recognition strategy for high sensitivity detection of chloramphenicol.

In this paper, an electrochemical sensor based on a dual recognition strategy of molecularly imprinted polymers (MIPs) and aptamer (Apt) has been designed for the high sensitivity detection of chloramphenicol (CAP). Here, MIPs and Apt have provided dual recognition sites to greatly improve the specific recognition ability of the sensor. Chitosan-multi-walled carbon nanotubes (CS-MWNTs) and AuNPs (gold nanoparticles) have been used for their excellent electrical conductivity.

A dual-mode biosensor featuring single-atom Fe nanozyme for multi-pesticide detection in vegetables.

The single-atom iron nanozyme (SA-Fe-NZ) exhibits high catalytic activity and excellent electron transfer efficiency in biosensors. However, the binding of bioreceptors to the surface of SA-Fe-NZ results in a decrease in the catalytic activity of the nanozyme due to its toxic effects. We utilized the toxic effects and excellent electrochemical properties of the SA-Fe-NZ to successfully construct a smartphone-assisted dual-mode biosensor.

Portable multichannel detection instrument based on time-resolved fluorescence immunochromatographic test strip for on-site detecting pesticide residues in vegetables.

In this work, a portable multichannel detection instrument based on time-resolved fluorescence immunochromatographic test strip (TRFIS) was proposed for on-site detecting pesticide residues in vegetables. Its hardware consisted of a silicon photodiode and excitation light source array, a mainboard of the lower machine with STMicroelectronics 32 (STM32) and a linear stepping motor. While detecting, cardboard with 6-channel TRFIS was pulled into the cassette by the stepping motor.

A multivariate algorithm for identifying contaminated peanut using visible and near-infrared hyperspectral imaging.

In this study, a novel uniform manifold approximation and projection combined-improved simultaneous optimization genetic algorithm-convolutional neural network (UMAP-ISOGA-CNN) algorithm was proposed. The improved simultaneous optimization genetic algorithm (ISOGA) combined with convolutional neural network (CNN) to optimize the architecture, hyperparameters, and optimizer of the CNN model simultaneously. Additionally, a uniform manifold approximation and projection (UMAP) method was used to visualize the feature space of all feature layers of the ISOGA-CNN model.

Paper-based biosensors relying on core biological immune scaffolds for the detection of procymidone in vegetables.

In order to achieve a highly sensitive detection of procymidone in vegetables, three paper-based biosensors based on a core biological immune scaffold (CBIS) were developed, which were time-resolved fluorescence immunochromatography strips with Europium (III) oxide (Eu-TRFICS). Goat anti-mouse IgG and europium oxide time-resolved fluorescent microspheres formed secondary fluorescent probes. CBIS was formed by secondary fluorescent probes and procymidone monoclonal antibody (PCM-Ab).

Spatio-temporal distribution patterns and quantitative detection of aflatoxin B and total aflatoxin in peanut kernels explored by short-wave infrared hyperspectral imaging.

Aflatoxin contamination in peanut kernels seriously harms the health of humans and causes significant economic losses. Rapid and accurate detection of aflatoxin is necessary to minimize its contamination. However, current detection methods are time-consuming, expensive and destructive to samples.

Label-free colorimetric apta-assay for detection of based on gold nanoparticles with peroxidase-like amplification.

In this work, aptamers against with better performance were obtained cell systematic evolution of ligands by exponential enrichment (cell-SELEX) and dissociation constants (Kd) of aptamers were estimated to range from 133.87 to 199.44 nM.

Multiplex strategy electrochemical platform based on self-assembly dual-site DNA tetrahedral scaffold for one-step detection of diazinon and profenofos.

In the work, based on self-assembly dual-site DNA tetrahedral scaffold (DTS), thionine (Thi), and 6-(Ferrocenyl)hexanethiol (Fc6S), a multiplex strategy electrochemical platform was fabricated for the simultaneous detection of profenofos (PFF) and diazinon (DZN). Thi and Fc6S were used to label aptamers for the synthesis of probes respectively. Notably, Thi and Fc6S engendered recognizable DPV peaks at different potentials to achieve simultaneous detection of PFF and DZN.

Portable electrochemiluminescence detection system based on silicon photomultiplier single photon detector and aptasensor for the detection of tetracycline in milk.

In this work, a portable electrochemiluminescence (ECL) detection system based on silicon photomultiplier (SiPM) single photon detector was proposed for the detection of ECL signals on a screen-printed electrode (SPE). This instrument innovatively used SiPM single photon detector to detect the ECL signal, which solved friability and bloat caused by the high operating voltage and the limitation of detection components in the traditional ECL detection instrument. This detection instrument showed excellent electrochemical and ECL detection performance.

Interference-resistant aptasensor with tetrahedral DNA nanostructure for profenofos detection based on the composites of graphene oxide and polyaniline.

In this work, an interference-resistant electrochemical aptasensor that could detect profenofos in vegetables was constructed based on complexes of graphene oxide and polyaniline (GO@PANI) and gold nanoparticles-tetrahedral DNA nanostructure (Au-TDN). Compared with a single chain aptamer, the tetrahedral DNA nanostructure is highly stable and allows the aptamer on this structure to stand in a highly ordered position on an electrode surface. Moreover, the AuNPs are biocompatible and can protect the activity of the aptamer, which can improve the assembly success rate of Au-TDN.

Ultrasensitive electrochemiluminescence aptasensor based on ABEI reduced silver nanoparticles for the detection of profenofos.

An ultrasensitive electrochemiluminescence (ECL) aptasensor for detection of profenofos was constructed by the reducibility and chemiluminescence property of N-(aminobutyl)-N-(ethylisoluminol) (ABEI). ABEI was used to reduce silver nitrate (AgNO) to silver nanoparticles (AgNPs), which could be adsorbed on the lattice of graphene oxide (GO) to form ABEI-AgNPs-GO complex. This compound could achieve excellent luminescence.