Effects of nonalcoholic fatty liver disease on sarcopenia: evidence from genetic methods.

With the aging of the population, sarcopenia has become more common. Studies have shown a broad association between liver disease and sarcopenia. However, this link remains unclear.

Integration of multiomics analysis to reveal the major pathways of vitamin A deficiency aggravates acute respiratory distress syndrome in neonatal rats.

Acute respiratory distress syndrome (ARDS) is a major disease that threatens the life and health of neonates. Vitamin A (VA) can participate in early fetal lung development and affect lung immune function. Researches revealed that the serum VA level in premature infants with ARDS was lower than that in premature infants without ARDS of the same gestational age, and premature infants with VA deficiency (VAD) were more likely to develop ARDS.

An inverse causal association between genetically predicted vitamin D and chronic obstructive pulmonary disease risk.

Aim: Observational studies have reported that levels of vitamin D were associated with the incidence of chronic obstructive pulmonary disease (COPD), but the relationship between them may have been confounded in previous studies. In this study, we aimed to determine the relationship between the levels of 25-hydroxyvitamin D (25OHD) and the risk of COPD by two-sample Mendelian randomization (MR) analysis.

Methods: Summary statistics for 25OHD and COPD in this study were obtained from the EBI ( = 496,946) consortium and Finn ( = 187,754) consortium.

Corrigendum: Genetically predicted C-reactive protein mediates the association between rheumatoid arthritis and atlantoaxial subluxation.

[This corrects the article DOI: 10.3389/fendo.2022.

Genetically predicted obesity and risk of hip osteoarthritis.

Objectives: To determine the causal association between genetically predicted obesity and the risk of hip osteoarthritis.

Methods: We performed two-sample Mendelian randomization (MR) analysis to analyze the association between body mass index (BMI) and hip osteoarthritis using pooled-level genome-wide association study (GWAS) data. The inverse variance weighted (IVW), MR‒Egger, and weighted median methods were used to estimate the causal association.

Genetically predicted C-reactive protein mediates the association between rheumatoid arthritis and atlantoaxial subluxation.

Objective: Investigating the causal relationship between rheumatoid arthritis (RA) and atlantoaxial subluxation (AAS) and identifying and quantifying the role of C-reactive protein (CRP) as a potential mediator.

Methods: Using summary-level data from a genome-wide association study (GWAS), a two-sample Mendelian randomization (MR) analysis of genetically predicted rheumatoid arthritis (14,361 cases, and 43,923 controls) and AAS (141 cases, 227,388 controls) was performed. Furthermore, we used two-step MR to quantitate the proportion of the effect of c-reactive protein-mediated RA on AAS.

Biological activity of galacto-oligosaccharides: A review.

Galacto-oligosaccharides (GOS) are oligosaccharides formed by β-galactosidase transgalactosylation. GOS is an indigestible food component that can pass through the upper gastrointestinal tract relatively intact and ferment in the colon to produce short-chain fatty acids (SCFAs) that further regulate the body's intestinal flora. GOS and other prebiotics are increasingly recognized as useful food tools for regulating the balance of colonic microbiota-human health.

Causal Effects of Genetically Predicted Cystatin C on Osteoporosis: A Two-Sample Mendelian Randomization Study.

Although it has long been reported that high levels of cystatin C could contribute to the development of osteoporosis in some studies, no evidence has established a causal association between them thus far. A Mendelian randomization (MR) study was conducted to determine the causal effect of cystatin C on osteoporosis based on public databases obtained from separately published genome-wide association studies (GWASs). The single-nucleotide polymorphisms (SNPs) for cystatin C were extracted from the MR-Base (CKDGen, 33,152 participants), and the SNPs for osteoporosis were extracted from the United Kingdom Biobank project (United Kingdom Biobank, including 5,266 osteoporosis cases and 331,893 controls).

Alleviation of Diabetic Tendon Injury via Activation of Tendon Fibroblasts Autophagy under Berberine Treatment.

Berberine (BBR) is an isoquinoline alkaloid isolated from Coptis chinensis and possesses valuable pharmacological activities, including anti-inflammatory, anti-tumor, and alleviating several complications of type 2 diabetes mellitus (T2DM). However, the role of BBR in regulating diabetic tendon injury remains poorly understood. In this study, a rat model of T2DM was constructed, and cell apoptosis and autophagy were assessed in tendon tissues after BBR treatment through TdT-Mediated dUTP nick-end labeling (TUNEL) assay and immunohistochemical analysis.

High glucose represses the proliferation of tendon fibroblasts by inhibiting autophagy activation in tendon injury.

Diabetic foot ulcer (DFU) is a kind of common and disabling complication of Diabetes Mellitus (DM). Emerging studies have demonstrated that tendon fibroblasts play a crucial role in remodeling phase of wound healing. However, little is known about the mechanism underlying high glucose (HG)-induced decrease in tendon fibroblasts viability.

Ameliorative effects of the traditional Chinese medicine formula on arteriosclerosis obliterans in a rabbit model.

Context: (QMY) is a clinically used herbal formula for treating arteriosclerosis obliterans (ASO).

Objective: To evaluate the chemical constituents and effects of QMY on ASO rabbit model.

Materials And Methods: Forty-eight New Zealand rabbits were divided into six groups ( = 8): normal (normal rabbits treated with 0.

[In vivo high-resolution imaging of rat retina with optical coherence tomography and the expression of Bcl-2, Bax, Caspase-3 mRNA during critical period plasticity].

Objective: To observe the changes of retinal thickness during critical period plasticity in rat, and to investigate whether apoptosis participates in the structural forming of retina.

Methods: Experimental research. 50 normal newborn pups of SD rat were randomly selected in the experiment.

F-heparin modified intraocular lenses in Rhesus monkeys.

Aim: In order to improve the biocompatibility of intraocular lenses (IOL), the polymethylmethacrylate (PMMA) IOL was modified with F-heparin.

Methods: The PMMA IOL was modified with F ions and heparin by the technique of ion beam combined with low temperature and low pressure plasma. The monkeys (20 eyes) with cataract partly were randomly classified into 2 groups and implanted with PMMA IOL and modified IOL respectively for 180 days.

[Combined rapamycin eye drop in nanometer vector and poly (lactic acid) wafers of cyclosporine A effectively prevents high-risk corneal allograft rejection in rabbits].

Objective: To evaluate the combined effect of topical rapamycin (RAPA) eye drop in nanometer vector and poly (lactic acid) (PLA) wafers of cyclosporine A (CsA) in the prevention of acute allograft rejection after rabbit corneal transplantation. Methods It was an experimental study. RAPA was incorporated into the nanometer particles and CsA was incorporated into PLA wafers.

Effects of bone-marrow mesenchymal stem cells transplanted into vitreous cavity of rat injured by ischemia/reperfusion.

Objective: To examine the survival, migration, integration, differentiation and the expression of various neurotrophic factors of bone-marrow mesenchymal stem cells (BMSCs) transplanted into the vitreous cavity of rats injured by ischemia/reperfusion(I/R).

Methods: The BMSCs were separated from rat marrow using the wall-sticking method, and cultured in vitro to expand. Flow cytometry detected the surface antigens of BMSCs.

[Early clinical study of bimanual microphacoemulsification with rollable intraocular lens].

Objective: To analyze the results of bimanual microphacoemulsification combined with rollable intraocular lens (IOL), to evaluate the feasibility and safety of this procedure and to compare the clinical results of two different types of rollable intraocular lens.

Methods: Thirty-five cases (35 eyes) were divided into 2 groups. Group 1 included 15 eyes used Thinoptx IOL, Group 2 included 20 eyes used Acri.

[Experimental study of spherical aberrations of intraocular lenses with different materials and designs].

Objective: To study the characteristics of spherical aberrations (SA) of the traditional intraocular lenses (IOLs) produced by different materials and designs.

Methods: A special optical metrical system was established in the laboratory to measure the SA of IOLs by using the Hartmann-Shack aberrometer. Eight different types of IOLs were divided into two Groups.

[Distribution and morphological changes of corneal nerves in type 2 diabetic patients detected by confocal microscopy].

Objective: To evaluate corneal nerve distribution and morphological changes in subjects with type 2 diabetes.

Methods: Sixty-five eyes of 59 patients with type 2 diabetes (26 males, 39 females) and 26 eyes (10 males, 16 females) of control subjects were included in the present study. Based on the indirect ophthalmoscopic examination and fluorescein angiography, the diabetic group was divided into three sub-groups according to the stage of retinopathy: without diabetic retinopathy (NDR), non-proliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy (PDR).

[Cloning, expression and cytotoxin to human lens epithelial cells of targeting toxin DT389-hbFGF].

Objective: To construct pGEX-DT(389)-hbFGF plasmid, express and identify the cytotoxicity to human lens epithelial cells (HLECs).

Methods: Extracting DNA of dead diphtheria bacillus and RNA of 12-week fetal brain cortex. The fragments of truncated diphtheria toxin (containing 389 amino acids of N-terminus, DT(389)) and full length human bFGF gene (encoding 18kd protein) were amplified by PCR technique respectively.

[Application of wavefront aberration technique in the study of phakic and pseudophakic eyes].

Modern cataract surgery is routinely combined with the implantation of an intraocular lens (IOL). Although surgical technique as well as IOL materials and designs have been improved dramatically, the problems related postoperative visual acuity still occur, such as glare disability, low visual acuity at night, and so on. Wavefront aberration technique can be used to help us revealing the real reasons of such postoperative visual problems.

Comparison of cell-suspension and explant culture of rabbit limbal epithelial cells.

Currently, most investigators directly use limbal explants to culture corneal epithelial cells. However, it has not been identified that limbal stem cells do readily migrate from the limbal explants onto culture plate or amniotic membrane carrier. In this study a cell-suspension culture system for rabbit limbal stem cells was developed and compared with the direct explant method in the aspect of stem cells content in the culture system.

A 1-year study on carbon, titanium surface-modified intraocular lens in rabbit eyes.

Purpose: To evaluate biocompatibility of Carbon (C) and Titanum (T) surface modified Polymethyl-methacrylate (PMMA) Intraocular Lens (IOLs) in pseudophakic rabbit model.

Methods: Extracapsule Cataract Extraction (ECCE) and IOLs implantation were performed in Japanese albino rabbit eyes. The white cell concentration from the aqueous humor in the eyes was counted within 3 months post operation.

Contrast sensitivity and color vision with a yellow intraocular len.

Objective: To evaluate contrast sensitivity and color vision of yellow ultraviolet (UV) intraocular len (IOL) in cataract patients.

Design: Randomized clinical trial.

Methods: Extracapsular cataract extraction was performed in 60 senile cataract patients.

Sex hormones and their receptors in patients with age-related cataract.

Purpose: To determine the sex hormone levels in the serum and aqueous humor in patients with age-related cataract and the corresponding hormone receptors in cataract lens epithelial cells (LECs).

Setting: Research Laboratory, International Intraocular Implant Training Center, Tianjin Medical University, Tianjin, China.

Methods: Serum and aqueous humor were drawn from patients with cataract and a control group.

[A comparison of clinical effects between phacoemulsification and intraocular lens implantation through "L-shaped" and 3.2 mm scleral tunnel incision].

Objective: To study the implant surgery through a newly designed L-shaped sutureless scleral tunnel incision and compare the therapeutic effects of this incision with the conventional 3.2 mm scleral tunnel incisions.

Methods: Phacoemulsification was performed through a L-shaped incision with implantation of 6 mm PMMA intraocular lens (IOL) and 3.