Improving cabbage resistance to via crosses with .

Unlabelled: Cabbage is a widely cultivated leafy vegetable, but head rot disease caused by the fungus can seriously reduce its yield and quality. There are currently not any cabbage varieties that are completely immune to the disease, but its wild relative is very resistant. In this study, cabbage resistance was improved by backcrossing a highly resistant accession (C01) with a susceptible cabbage cultivar (F416).

Comparative Analysis of Transcriptomes Reveals Pathways and Verifies Candidate Genes for Clubroot Resistance in .

Clubroot, a soil-borne disease caused by , is one of the most destructive diseases of all over the world. However, the mechanism of clubroot resistance remains unclear. In this research, transcriptome sequencing was conducted on root samples from both resistant (R) and susceptible (S) plants infected by .

Comparative Transcriptome Analysis Points to the Biological Processes of Hybrid Incompatibility between and .

Improving via introgression of the genome components from its parental species, and , is an important breeding strategy. Interspecific hybridization between and is compatible with high rate of survival ovules, while the hybridization between and is incompatible with the high occurrence of embryo abortion. To understand the diverse embryo fate in the two interspecific hybridizations, here, the siliques of pollinated with (AE) and (NE) were employed for transcriptome sequencing at 8 and 16 days after pollination.

Genetic analysis and QTL mapping for silique density in rapeseed (Brassica napus L.).

Genetic models, QTLs and candidate gene for silique density on main inflorescence of rapeseed were identified. Silique density is one of the critical factors to determine seed yield and plant architecture in rapeseed (Brassica napus L.); however, the genetic control of this trait is largely unknown.

Selective modes affect gene feature and function differentiation of tetraploid species in their evolution and domestication.

The genus contains a diverse group of important vegetables and oilseed crops. Genome sequencing has been completed for the six species (, , , , , and ) in U's triangle model. The purpose of the study is to investigate whether positively and negatively selected genes (PSGs and NSGs) affect gene feature and function differentiation of tetraploids in their evolution and domestication.

Identification of Candidate Genes for Clubroot-Resistance in Using Quantitative Trait Loci-Sequencing.

Clubroot caused by is a devastating disease of cabbage (). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in , genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed separately from 110 and 74 F2 cloned lines derived from the cross between clubroot-resistant (R) cabbage "GZ87" (against race 4) and susceptible (S) cabbage "263." Based on the QTL- (QTL-Seq) analysis of group I and group II, three QTLs (i.

Silicon Alleviates the Disease Severity of Sclerotinia Stem Rot in Rapeseed.

Sclerotinia stem rot, caused by , is a devastating disease in rapeseed. The objective of this study was to investigate the role and the mechanism of silicon (Si) in alleviating the disease severity of in rapeseed. In the absorption assays, the rapeseed that absorbed 10 mM of KSiO exhibited an 86% decrease in lesion size on infected leaves as compared with controls.

Investigating genetic relationship of with via virtual allopolyploidy and hexaploidy strategy.

Unlabelled: is an important economic crop of the world; however, the narrow genetic base of this crop has tremendously decreased its crop productivity. As an ancestral species of , is of great importance in widening the genetic diversity of . In the present study, 42 SSR markers were employed to screen the genetic diversity among 83 , 16 , and other Brassica accessions.

Sclerotinia sclerotiorum utilizes host-derived copper for ROS detoxification and infection.

Necrotrophic plant pathogen induces host reactive oxygen species (ROS) production, which leads to necrosis in the host, allowing the pathogen to absorb nutrients from the dead tissues. Sclerotinia sclerotiorum is a typical necrotrophic pathogen that causes Sclerotinia stem rot in more than 400 species, resulting in serious economic losses. Here, we found that three S.

Introgression and pyramiding of genetic loci from wild Brassica oleracea into B. napus for improving Sclerotinia resistance of rapeseed.

Resistant rapeseed lines pyramided with multiple resistant QTLs derived from Brassica oleracea were developed via a hexaploidy strategy. Rapeseed (Brassica napus L.) suffers heavily from Sclerotinia stem rot, but the breeding of Sclerotinia-resistant rapeseed cultivar has been unsuccessful.

Understanding the Resistance Mechanism in to Clubroot Caused by .

Exploring the mechanism of plant resistance has become the basis for selection of resistance varieties but reports on revealing resistant mechanism in against are rare. In this study, RNA-seq was conducted in the clubroot-resistant breeding line ZHE-226 and in the clubroot-susceptible rapeseed cultivar Zhongshuang 11 at 0, 3, 6, 9, and 12 days after inoculation. Strong alteration was detected specifically in ZHE-226 as soon as the root hair infection happened, and significant promotion was found in ZHE-226 on cell division or cell cycle, DNA repair and synthesis, protein synthesis, signaling, antioxidation, and secondary metabolites.

Simultaneous Transcriptome Analysis of Host and Pathogen Highlights the Interaction Between Brassica oleracea and Sclerotinia sclerotiorum.

White mold disease caused by Sclerotinia sclerotiorum is a devastating disease of Brassica crops. Here, we simultaneously assessed the transcriptome changes from lesions produced by S. sclerotiorum on disease-resistant (R) and -susceptible (S) B.

Genome-wide identification of loci affecting seed glucosinolate contents in Brassica napus L.

Glucosinolates are amino acid-derived secondary metabolites that act as chemical defense agents against pests. However, the presence of high levels of glucosinolates severely diminishes the nutritional value of seed meals made from rapeseed (Brassica napus L.).

Genetic characterization and fine mapping for multi-inflorescence in Brassica napus L.

A major QTL for multi-inflorescence was mapped to a 27.18-kb region on A05 in Brassica napus by integrating QTL mapping, microarray analysis and whole-genome sequencing. Multi-inflorescence is a desirable trait for the genetic improvement of rapeseed (Brassica napus L.

Identification of Quantitative Trait Loci for Clubroot Resistance in With the Use of SNP Microarray.

Increasing clubroot resistance (CR) of by ascertaining the molecular mechanisms has been the key focus in modern breeding. In order to identify the quantitative trait loci (QTLs) associated with CR in , 94 F2 vegetative lines which were developed by tissue culture of selfed seeds from the F1 generation between a clubroot-resistant inbred line and a susceptible line, were identified for disease incidence and six CR-associated traits under a lab inoculation by and were genotyped with the 60K SNP array. Significant correlations were detected for numbers of fibrous roots and content in roots with disease incidence.

Mapping of genetic locus for leaf trichome in Brassica oleracea.

The genetic locus for leaf trichome was identified via marker-based mapping and SNP microarray assay, and a functional marker was developed to facilitate the breeding for hairiness in Brassica oleracea. Plant trichomes are involved in various functions particularly in protecting plants against some biotic and abiotic damages. In the present study, an F2 segregating population was developed from the cross between a glabrous cultivated B.

Time-Series Analyses of Transcriptomes and Proteomes Reveal Molecular Networks Underlying Oil Accumulation in Canola.

Understanding the regulation of lipid metabolism is vital for genetic engineering of canola ( L.) to increase oil yield or modify oil composition. We conducted time-series analyses of transcriptomes and proteomes to uncover the molecular networks associated with oil accumulation and dynamic changes in these networks in canola.

Transcriptomic comparison between Brassica oleracea and rice (Oryza sativa) reveals diverse modulations on cell death in response to Sclerotinia sclerotiorum.

Sclerotinia stem rot caused by Sclerotinia sclerotiorum is a devastating disease of Brassica crops, but not in rice. The leaves of a rice line, a partial resistant (R) and a susceptible (S) Brassica oleracea pool that bulked from a resistance-segregating F2 population were employed for transcriptome sequencing before and after inoculation by S. sclerotiorum for 6 and 12 h.

Ss-Rhs1, a secretory Rhs repeat-containing protein, is required for the virulence of Sclerotinia sclerotiorum.

Sclerotinia sclerotiorum is a devastating necrotrophic plant pathogen with a worldwide distribution. Cell wall-degrading enzymes and oxalic acid are important to the virulence of this pathogen. Here, we report a novel secretory protein, Ss-Rhs1, which is essential for the virulence of S.

Comparative quantitative trait loci for silique length and seed weight in Brassica napus.

Silique length (SL) and seed weight (SW) are important yield-associated traits in rapeseed (Brassica napus). Although many quantitative trait loci (QTL) for SL and SW have been identified in B. napus, comparative analysis for those QTL is seldom performed.

Development of genic cleavage markers in association with seed glucosinolate content in canola.

The orthologues of Arabidopsis involved in seed glucosinolates metabolism within QTL confidence intervals were identified, and functional markers were developed to facilitate breeding for ultra-low glucosinolates in canola. Further reducing the content of seed glucosinolates will have a positive impact on the seed quality of canola (Brassica napus). In this study 43 quantitative trait loci (QTL) for seed glucosinolate (GSL) content in a low-GSL genetic background were mapped over seven environments in Germany and China in a doubled haploid population from a cross between two low-GSL oilseed rape parents with transgressive segregation.

Transfer of sclerotinia resistance from wild relative of Brassica oleracea into Brassica napus using a hexaploidy step.

Sclerotinia resistance was transferred into rapeseed from a wild relative of Brassica oleracea (B. incana) using hexaploids derived from crosses between B. incana and rapeseed as a bridge.

DNA Methylation Alterations at 5'-CCGG Sites in the Interspecific and Intraspecific Hybridizations Derived from Brassica rapa and B. napus.

DNA methylation is an important regulatory mechanism for gene expression that involved in the biological processes of development and differentiation in plants. To investigate the association of DNA methylation with heterosis in Brassica, a set of intraspecific hybrids in Brassica rapa and B. napus and interspecific hybrids between B.

A large-scale introgression of genomic components of Brassica rapa into B. napus by the bridge of hexaploid derived from hybridization between B. napus and B. oleracea.

Brassica rapa (AA) has been used to widen the genetic basis of B. napus (AACC), which is a new but important oilseed crop worldwide. In the present study, we have proposed a strategy to develop new type B.