Molecular Characterization and Pathogenicity Analysis of Porcine Rotavirus A.

Porcine rotavirus A (RVA) is one of the major etiological agents of diarrhea in piglets and constitutes a significant threat to the swine industry. A molecular epidemiological investigation was conducted on 2422 diarrhea samples from Chinese pig farms to enhance our understanding of the molecular epidemiology and evolutionary diversity of RVA. The findings revealed an average RVA positivity rate of 42% (943/2422), and the study included data from 26 provinces, primarily in the eastern, southern and southwestern regions.

Affinity Peptide-Based Circularly Permuted Fluorescent Protein Biosensors for Non-Small Cell Lung Cancer Diagnosis.

Non-small cell lung cancer (NSCLC) is the predominant form of lung cancer and poses a significant public health challenge. Early detection is crucial for improving patient outcomes, with serum biomarkers such as carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCCAg), and cytokeratin fragment 19 (CYFRA 21-1) playing a critical role in early screening and pathological classification of NSCLC. However, due to being mainly based on corresponding antibody binding reactions, existing detection technologies for these serum biomarkers have shortcomings such as complex operations, high false positive rates, and high costs.

Regional antimicrobial resistance gene flow among the One Health sectors in China.

Background: Antimicrobial resistance poses a significant threat to global health, with its spread intricately linked across human, animal, and environmental sectors. Revealing the antimicrobial resistance gene (ARG) flow among the One Health sectors is essential for better control of antimicrobial resistance.

Results: In this study, we investigated regional ARG transmission among humans, food, and the environment in Dengfeng, Henan Province, China by combining large-scale metagenomic sequencing with culturing of resistant bacterial isolates in 592 samples.

Single-cell RNA sequencing highlights the unique tumor microenvironment of small cell neuroendocrine cervical carcinoma.

Small cell neuroendocrine cervical carcinoma is a highly aggressive tumor characterized by early metastasis, a high recurrence rate, and poor prognosis. This study represents the first instance of single-cell sequencing conducted on small cell neuroendocrine carcinoma of the cervix worldwide. Analysis of gene expression regulatory networks revealed that the transcription factor TFF3 drived up-regulation of ELF3.

Distinguishing local isomorphism classes in quasicrystals by high-order harmonic spectroscopy.

Electron diffraction spectroscopy is a fundamental tool for investigating quasicrystal structures, which unveils the quasiperiodic long-range order. Nevertheless, it falls short in effectively distinguishing separate local isomorphism classes. This is a long outstanding problem.

Mmu_circ_0001148 promotes endothlial-mesenchymal transition via regulating miR-218-5p/JMY axis and drives progression of atherosclerosis.

Atherosclerosis (AS) is a common cardiovascular disease and responsible for the high mortality of cardiovascular emergencies. Circular RNAs (circRNAs) show a potential role in atherogenesis. We identified an aberrantly expressed circRNA (circ_0001148) in atherosclerotic tissues.

Dynamic reconfiguration of multiobjective distribution networks considering the variation of load and DG using a novel LDEDBO algorithm.

To address the challenges related to active power dissipation and node voltage fluctuation in the practical transformation of power grids in the field of new energy such as wind and photovoltaic power generation, an improved Dung Beetle Optimization Algorithm Based on a Hybrid Strategy of Levy Flight and Differential Evolution (LDEDBO) is proposed. This paper systematically addresses this issue from three aspects: firstly, optimizing the DBO algorithm using Chebyshev chaotic mapping, Levy flight strategy, and differential evolution algorithm; secondly, validating the algorithm's feasibility through real-time network reconfiguration at random time points within a 24-h period; and finally, applying the LDEDBO to address the dynamic reconfiguration problems of the IEEE-33 and IEEE-69 node bus. The simulation indicates that the power dissipation of the IEEE-33 node bus is decreased by 28.

ADSCs-derived exosomes suppress macrophage ferroptosis via the SIRT1/NRF2 signaling axis to alleviate acute lung injury in sepsis.

Acute lung injury being one of the earliest and most severe complications during sepsis and macrophages play a key role in this process. To investigate the regulatory effects and potential mechanisms of adipose mesenchymal stem cell derived-exosomes (ADSC-exo) on macrophages and septic mice, ADSCs-exo was administrated to both LPS-induced macrophage and cecal ligation and puncture (CLP) induced sepsis mice. ADSCs-exo was confirmed to inhibit M1 polarization of macrophages and to reduce excessive inflammation.

DYRK4 upregulates antiviral innate immunity by promoting IRF3 activation.

Viral infection activates the transcription factors IRF3 and NF-κB, which induce type I interferon (IFN) and antiviral innate immune responses. Here, we identify dual-specific tyrosine phosphorylation-regulated kinase 4 (DYRK4) as an important regulator of virus-triggered IFN-β induction and antiviral innate immunity. Overexpression of DYRK4 enhances virus-triggered activation of IRF3 and type I IFN induction, whereas knockdown or knockout of DYRK4 impairs virus-induced activation of IRF3 and NF-κB.

Deficiency of the flagellin subunit FliC exacerbates the pathogenicity of extraintestinal pathogenic Escherichia coli in BALB/c mice by inducing a more intense inflammation.

Extraintestinal pathogenic Escherichia coli (ExPEC) can cause systemic infections in livestock and poultry. Flagellin, a classical virulence factor, acts as a promoter of cell adhesion and invasion, as well as an inducer of inflammatory responses during intestinal pathogen infection. Further understanding is needed regarding the interaction between flagellin and host within the extra-intestinal ecological niche to facilitate a deeper comprehension of ExPEC infection mechanisms.

Effects of ultrasound-assisted plasma-activated water pretreatment combined with electrohydrodynamics on drying characteristics, active ingredients and volatile components of yam (Dioscorea opposita).

This paper explores the effect of ultrasound (US) assisted plasma-activated water (PAW) or deionized water (DW) pretreatment combined with electrohydrodynamics (EHD) on the drying of yam. The activity characteristics of four pretreatments (plasma activated water combined with ultrasound (PAW + US), plasma activated water (PAW), deionized water combined with ultrasound (DW + US), and deionized water (DW) (control)) and their effects on drying characteristics, rehydration rate, color, reducing sugars, total phenols, infrared spectra, and volatile compositions of yam under EHD drying process were investigated. The results showed that the media pretreaded by ultrasound (US) combined with plasma-activated water (PAW) has lower media of pH (53.

Autologous DNA mobilization and multiplication expedite natural products discovery from bacteria.

The transmission of antibiotic-resistance genes, comprising mobilization and relocation events, orchestrates the dissemination of antimicrobial resistance. Inspired by this evolutionarily successful paradigm, we developed ACTIMOT, a CRISPR-Cas9-based approach to unlock the vast chemical diversity concealed within bacterial genomes. ACTIMOT enables the efficient mobilization and relocation of large DNA fragments from the chromosome to replicative plasmids within the same bacterial cell.

Influence of ultrafast laser processing on amorphous structures - based on molecular dynamics simulation.

Ultrafast laser processing technology exhibits exceptional precision and irreplaceable functionality in the fabrication of micron and nanometer-scale devices. However, its short action time presents challenges for observing and studying the interactions between ultrafast lasers and materials. This study employs molecular dynamics simulations to specifically investigate the application of ultrafast laser processing in treating amorphous structural defects on Ni-Fe alloy surfaces.

High temperature induces the upward shift of the thermal neutral zone and decreases metabolic capacity in zebra finches.

The thermal neutral zone (TNZ) represents a fundamental concept in the thermal physiology of homeothermic organisms. TNZ is characterized as a specific range of environmental temperatures within which the metabolic rate remains at its basal level. The ambient temperature is regarded as a critical environmental factor that affects an animal's thermoregulation and propels the development of various morphological, physiological, and behavioral adaptations.

A "Janus" Zwitterionic Hydrogel Patch for Tissue Repair and Prevention of Post-Operative Adhesions.

Anti-peritoneal adhesions (PA) are very important after abdominal surgery for that PA often leads to other medical problems and imposes a huge financial burden on the national healthcare system. In this work, a "Janus" zwitterionic hydrogel patch where one side can adhere firmly to the tissue, while the other side has anti-fouling properties and has little interaction with the surrounding tissue has been developed. The "Janus" hydrogel patch is prepared by in situ formation of a bonding polymer layer poly(acrylic-co-N-hydroxysuccinimide acrylate) on one side of zwitterionic hydrogel.

[Research Progress and Perspectives of Antibody-drug Conjugates Targeting Trophoblast Cell Surface Antigen-2 in Advanced Non-small Cell Lung Cancer].

Non-small cell lung cancer (NSCLC) remains a significant global health burden, and there is an urgent need for new treatment options. Trophoblast cell surface antigen-2 (TROP-2), a target closely associated with NSCLC prognosis, has become a research hotspot in recent years. Notably, TROP-2-targeted antibody-drug conjugates (ADCs) have made groundbreaking advances in NSCLC therapy.

4'-Demethylpodophyllotoxin functions as a mechanism-driven therapy by targeting the PI3K-AKT pathway in Colorectal cancer.

The treatment of colorectal cancer (CRC) poses significant challenges in terms of drug resistance and poor prognosis, necessitating the exploration of effective therapeutic strategies. In this study, high-throughput drug screening was utilized to identify Chinese herbal medicines with notable therapeutic effects on CRC. Among the compounds identified, 4'-demethylpodophyllotoxin (DOP), a derivative of podophyllotoxin, emerged as a potent anti-cancer compound.

Disease-specific suppressive granulocytes participate in glioma progression.

Glioblastoma represents one of the most aggressive cancers, characterized by severely limited therapeutic options. Despite extensive investigations into this brain malignancy, cellular and molecular components governing its immunosuppressive microenvironment remain incompletely understood. Here, we identify a distinct neutrophil subpopulation, termed disease-specific suppressive granulocytes (DSSGs), present in human glioblastoma and lower-grade gliomas.

Designing NiMnSn Heusler magnetic nanoprecipitate in copper alloy for increased strength and electromagnetic shielding.

Structural electromagnetic shielding materials are required to withstand high stress and electromagnetic interference in extreme environments. In this paper, a nano-magnetic Heusler phase with desired structure parameters was successfully obtained in a copper matrix by employing a multi-objective driving design strategy. The resulting copper alloy exhibits a yield strength of up to 1.

BS-CP: Efficient streaming Bayesian tensor decomposition method via assumed density filtering.

Tensor data is common in real-world applications, such as recommendation system and air quality monitoring. But such data is often sparse, noisy, and fast produced. CANDECOMP/PARAFAC (CP) is a popular tensor decomposition model, which is both theoretically advantageous and numerically stable.

Proanthocyanidins alleviate acute alcohol liver injury by inhibiting pyroptosis via inhibiting the ROS-MLKL-CTSB-NLRP3 pathway.

Background: Alcoholic Liver Disease (ALD) is a hepatic disorder resulting from prolonged or excessive alcohol intake. The predominant manifestation of ALD is fatty liver, which progresses to alcoholic hepatitis as the disease worsens. Pyroptosis is a novel type of programmed cell death that is intricately linked to the inflammatory cascade, presenting a promising avenue for therapeutic intervention in the management of ALD.

A Polycarbonate-Assisted Transfer Method for van der Waals Contacts to Magnetic Two-Dimensional Materials.

Magnetic two-dimensional (2D) materials have garnered significant attention for their potential to revolutionize 2D spintronics due to their unique magnetic properties. However, their air-sensitivity and highly insulating nature of the magnetic semiconductors present substantial challenges for device fabrication with effective contacts. In this study, we introduce a polycarbonate (PC)-assisted transfer method that effectively forms van der Waals (vdW) contacts with 2D materials, streamlining the fabrication process without the need for additional lithography.

Enhancing β-Galactosidase Performance for Galactooligosaccharides Preparation via Strategic Glucose Re-Tunneling.

This study focuses on the characterization and re-engineering of glucose transport in β-galactosidase (BglD) to enhance its catalytic efficiency. Computational prediction methods were employed to identify key residues constituting access tunnels for lactose and glucose, revealing distinct pockets for both substrates. In silico simulated saturation mutagenesis of residues T215 and T473 led to the identification of eight mutant variants exhibiting potential enhancements in glucose transport.

Assessment of the impact of quinoa protein modification on silver carp surimi gel quality and its utilization in 3D printing.

This study aims to investigate the effects of different modified quinoa proteins (MQP) on the gel quality of silver carp surimi, as well as to explore their feasibility for three-dimensional (3D) printing. Five different methods were employed to modify quinoa protein, water bath (WB), microwave (MW), ultrasonic (US), microwave followed by ultrasonic (M-U), and ultrasonic followed by microwave (U-M), to prepare compound surimi gels (MQPs). The results showed that the addition of quinoa protein improved the gel quality of surimi, while the MQP had an even better effect on enhancing the gel quality.