Study on the mechanism of Huangqi Chifeng decoction regulating ferroptosis inhibiting smooth muscle cells derived foam cell formation.

Ethnopharmacological Relevance: Chinese medicine, specifically Huangqi Chifeng Decoction (HQCF), is recognized for its efficacy in treating atherosclerosis (AS), a common cardiovascular disease. Despite its established benefits in addressing Qi deficiency, blood stasis, and collateral obstruction, the precise mechanism through which HQCF affects AS remains unclear.

Aim Of The Study: This study investigated the potential of HQCF to mitigate AS by suppressing smooth muscle cell (SMC) foam formation through the ferroptosis pathway.

Guizhi Fuling capsules can alleviate bortezomib-induced peripheral neuropathy by decreasing Interleukin-6 levels to regulate mTOR pathway-induced autophagy.

Objective: To investigate the therapeutic effect and underlying mechanism of Guizhi Fuling capsule (GZFL) on bortezomib-induced peripheral neuropathy (BiPN).

Materials And Methods: Interleukin-6 (IL-6) levels in the plasma of Multiple myeloma (MM) patients were measured by ELISA, and correlation analysis between IL-6 and clinical features of BiPNs was performed. Then, we assess the clinical therapeutic effects of GZFL on MM patients by detecting IL-6 level, PN grade, FACT score, VAS score, MVC and SCV before and after the treatment.

Long-term effects of an online mindfulness intervention on mental health in Chinese nursing students: a randomized controlled trial follow-up.

Objective: This study aims to evaluate the long-term impact of the six-week online mindfulness intervention course "Mindfulness Living With Challenge (MLWC)" on the mental health of undergraduate nursing students in Beijing, China, through a three-month follow-up.

Methods: A three-month follow-up was conducted upon completion of the six-week MLWC course. Depression-Anxiety-Stress Scale (DASS-21), Chinese Short Formed Five Facets of Mindfulness Questionnaire (FFMQ-SF), and the Perceived Social Support Scale (PSSS) were used to evaluate participants' mental health, mindfulness, and perceived social support.

Uptake and hesitancy of the second booster dose of COVID-19 vaccine among the general population in China after the surge period of the COVID-19 pandemic: a large-scale national study.

Background: Although the second booster dose of COVID-19 vaccines is available, vaccine hesitancy among the public may have peaked due to the surge in infections caused by the Omicron variant. To improve coverage of the second booster dose, it is crucial to investigate the prevalence of vaccine hesitancy among the general population during this period and explore the reasons for this phenomenon.

Methods: A cross-sectional survey was conducted between January 5 to February 9, 2023.

Tailoring SERS sensitivity in AgNWs-ZIF-67 substrates: Effects of MOF thickness and probe-pore size matching.

In this study, we develop a filter paper-supported Ag nanowire (AgNWs) substrate coated with a tunable layer of zeolitic imidazolate framework-67 (ZIF-67) metal-organic framework (MOF) for surface-enhanced Raman scattering (SERS) applications. The thickness of the ZIF-67 layer is precisely controlled by adjusting the soaking time, allowing us to investigate the influence of MOF thickness on SERS performance using probes with different sizes. Sensitivity for large, impenetrable molecules such as thiram and rhodamine 6G (R6G) are primarily governed by localized surface plasmon resonance (LSPR), while smaller, penetrable molecules like 4-mercaptobenzoic acid (4-MBA) exhibit enhanced sensitivity driven by charge transfer (CT) effects due to their ability to penetrate the MOF layer.

Genomic Landscape and Prediction of Udder Traits in Saanen Dairy Goats.

Goats are essential to the dairy industry in Shaanxi, China, with udder traits playing a critical role in determining milk production and economic value for breeding programs. However, the direct measurement of these traits in dairy goats is challenging and resource-intensive. This study leveraged genotyping imputation to explore the genetic parameters and architecture of udder traits and assess the efficiency of genomic prediction methods.

Prevalence and influencing factors of occupational burnout among healthcare workers in the Chinese mainland during the late 2022 Omicron COVID-19 outbreak: a multicenter cross-sectional study.

China witnessed an Omicron COVID-19 outbreak at the end of 2022. During this period, medical crowding and enormous pressure on the healthcare systems occurred, which might result in the occurrence of occupational burnout among healthcare workers (HCWs). This study aims to investigate the prevalence of occupational burnout and associated mental conditions, such as depressive symptoms, anxiety, PTSD symptoms, perceived social support, resilience, and mindfulness among HCWs of the Chinese mainland during the Omicron COVID-19 outbreak, and to explore the potential risk and protective factors influencing occupational burnout of HCWs.

Detection of differences in physical symptoms between depressed and undepressed patients with breast cancer: a study using K-medoids clustering.

Background: To detect the differences in physical symptoms between depressed and undepressed patients with breast cancer (BC), including common symptoms, co-occurring symptoms, and symptom clusters based on texts derived from social media and expressive writing.

Methods: A total of 1830 texts from social media and expressive writing were collected. The Chi-square test was used to compare the frequency of physical symptoms between depressed and undepressed patients with BC.

A Graph-based Goat Pangenome Reveals Structural Variations Involved in Domestication and Adaptation.

Pangenomes can facilitate a deeper understanding of genome complexity. Using de novo phased long-read assemblies of eight representative goat breeds, we constructed a graph-based pangenome of goats (Capra hircus) and discovered 113-Mb autosomal novel sequences. Combining this multi-assembly pangenome with low-coverage PacBio HiFi sequences, we constructed a long-read structural variations (SVs) database containing 59,325 SV deletions, 84,910 SV insertions, and 24,954 other complex SV alleles.

Intron , a Novel DNA Element, Has Global Effects on Synthetic Pathway Engineering by Empowering Protein Synthesis.

Classical genetic components in synthetic biology encompass essential elements of promoters, transcription factors, protein-coding genes, and terminators while both academic and industrial needs require novel engineering tools. Our study explores the potential of introns as versatile, novel biological DNA elements. Using intron from , the expression of was enhanced by 18.

Isoliquiritin targeting m5C RNA methylation improves mitophagy in doxorubicin-induced myocardial cardiotoxicity.

Background: Doxorubicin (DOX)-induced myocardial cardiotoxicity (DIC) severely limits its clinical application, and there is no optimal therapeutic agent available. Recent studies revealed that activation of BNIP3-mediated mitophagy and the inhibition of m5C RNA methylation played a crucial role in DIC. Isoliquiritin (ISL) has remarkable cardiac protective effect.

Histone Methylation, Energy Metabolism, and Alzheimer's Disease.

Alzheimer's disease (AD) is an insidious, progressive, and irreversible neurodegenerative disease characterized by the deposition of extracellular amyloid β-protein (Aβ) to form senile plaques and abnormal phosphorylation of intracellular tau protein to form neuronal fiber tangles. The pathogenesis of AD is complex, and there are several hypotheses, primarily including the Aβ cascade hypothesis, the neurofibrillary tangle hypothesis, the inflammatory hypothesis, and the cholinergic hypothesis. It has been suggested that the dysregulation of multiple energy metabolic pathways, especially mitochondria metabolism, may be related to the severity of AD pathology and disease symptoms in the brain.

Attitudes and Barriers to Patient Participation in Evidence-Based Practice From Chinese Nurses' Perspectives: A Qualitative Content Analysis.

Patient participation plays a vital role in evidence-based practice (EBP), yet its development in clinical nursing is slow. Current research has focused on identifying barriers to patient participation in everyday care, with less research on barriers to patient participation in EBP. Therefore, a questionnaire containing basic information and open-ended questions was used to explore nursing staff attitudes and barriers to patient participation in EBP.

m1A regulator‑mediated methylation modifications and gene signatures and their prognostic value in multiple myeloma.

N-methyladenosine (m1A), a methylation of RNA, is gaining attention for its role in diverse biological processes. However, the potential roles of m1A regulatory-mediated methylation modifications in multiple myeloma (MM) remain unclear. The mRNA expression of m1A regulators in normal plasma (NP; n=9) and MM (n=174) bone marrow plasma cells was investigated and the m1A modification patterns of 559 MM samples based on the expression of 10 m1A-related regulatory genes were comprehensively evaluated.

Study on Electrochemical Behaviors of Heat-Treated and .

Evaluating the bacterial activity effectively is critical to addressing the challenges posed by bacterial infections. Electrochemistry offers significant advantages in accuracy and cost efficiency compared with methods that are more time-consuming or require expensive instrumentation. This study initially established an electrochemical method for detecting bacterial activity using heat treatment as the pretreatment step.

Electrochemical response mechanism of DNA damaged cells: DNA damage repair and purine metabolism activation.

In modern society, due to the sharp increase in pollutants that cause DNA damage, there is a growing demand for innovative detection techniques and biomarkers. In this paper, the electrochemical behavior of HepG2 cells exposed to CdCl was investigated, and the electrochemical response mechanism of DNA damage was identified by exploring the correlation between the DNA damage response and purine metabolism. Western blot analysis revealed that the expression levels of ATM and Ku70 increased at 0.

Aberrant NSUN2-mediated m5C modification of exosomal LncRNA MALAT1 induced RANKL-mediated bone destruction in multiple myeloma.

The impact of exosome-mediated crosstalk between multiple myeloma (MM) cells and osteoclasts (OCs) on bone lesions remains to be investigated. Here, we identified NSUN2 and YBX1-mediated m5C modifications upregulated LncRNA MALAT1 expression in MM cells, which could be transported to OCs via exosomes and promote bone lesions. Methodologically, RNA-seq was carried out to detect the cargoes of exosomes.

Analysis of the Long Non-Coding and Messenger RNA Expression Profiles in the Skin Tissue of Super Merino and Small-Tailed Han Sheep.

Wool quality and yield are two important economic livestock traits. However, there are relatively few molecular studies on lncRNA for improving sheep wool, so these require further exploration. In this study, we examined skin tissue from the upper scapula of Super Merino (SM) and Small-Tailed Han (STH) sheep during the growing period.

Legionella maintains host cell ubiquitin homeostasis by effectors with unique catalytic mechanisms.

The intracellular bacterial pathogen Legionella pneumophila modulates host cell functions by secreting multiple effectors with diverse biochemical activities. In particular, effectors of the SidE family interfere with host protein ubiquitination in a process that involves production of phosphoribosyl ubiquitin (PR-Ub). Here, we show that effector LnaB converts PR-Ub into ADP-ribosylated ubiquitin, which is further processed to ADP-ribose and functional ubiquitin by the (ADP-ribosyl)hydrolase MavL, thus maintaining ubiquitin homeostasis in infected cells.

utilizes a unique type VI secretion system to promote its survival in niches with prey bacteria.

Pathogenic bacteria of the genus pose a severe threat to human health worldwide due to their strong adaptability, tolerance, and antibiotic resistance. Most isolates of these bacteria harbor a type VI secretion system (T6SS) that allows them to outcompete co-residing microorganisms, but whether this system is involved in acquiring nutrients from preys remains less studied. In this study, we found that Ab25, a clinical isolate of utilizes a T6SS to kill taxonomically diverse microorganisms, including bacteria and fungi.

Immune- and metabolism-related gene signature analysis uncovers the prognostic and immune microenvironments of hepatocellular carcinoma.

Background: Metabolic reprogramming is an emerging hallmark that influences the tumour microenvironment (TME) by regulating the behavior of cancer cells and immune cells. The relationship between metabolism and immunity remains elusive. The purpose of this study was to explore the predictive value of immune- and metabolism-related genes in hepatocellular carcinoma (HCC) and their intricate interplay with TME.