Publications by authors named "Jiaoyuan Fang"

Nanozymes, a class of nanomaterials mimicking natural enzymatic functions, have gained significant attention due to their exceptional biocatalytic properties and wide-ranging applications in biosensing. The total antioxidant capacity (TAC) can serve as a crucial parameter for assessing food quality, guiding dietary choices, and monitoring health conditions. In recent years, various nanomaterials with peroxidase (POD)-like and oxidase (OXD)-like activity have been widely used for TAC determination.

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A simple and reliable total antioxidant capacity (TAC) assay is essential for food safety evaluation and human health monitoring. Herein, a trimetallic nanozyme (FeO@Ag@Pt) was synthesized and exhibited OXD-, POD- and SOD-like activities, which could generate a synergistic catalytic system. FeO@Ag@Pt can catalyze oxygen to produce various reactive oxygen intermediates, and the endogenous product HO could be captured and further dissociated efficiently into •OH, due to its strong substrate binding affinity.

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Rapid and sensitive detection of nucleic acids has become crucial in various fields. However, most current nucleic acid detection methods can only be used in specific scenarios, such as RT-qPCR, which relies on fluorometer for signal readout, limiting its application at home or in the field due to its high price. In this paper, a universal nucleic acid detection platform combing CRISPR/Cas12a and strand displacement amplification (CRISPR-SDA) with multiple signal readout was established to adapt to different application scenarios.

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