Advances in total antioxidant capacity detection based on nanozyme.

Nanozymes, a class of nanomaterials mimicking natural enzymatic functions, have gained significant attention due to their exceptional biocatalytic properties and wide-ranging applications in biosensing. The total antioxidant capacity (TAC) can serve as a crucial parameter for assessing food quality, guiding dietary choices, and monitoring health conditions. In recent years, various nanomaterials with peroxidase (POD)-like and oxidase (OXD)-like activity have been widely used for TAC determination.

FeO@Ag@Pt nanoparticles with multienzyme like activity for total antioxidant capacity assay.

A simple and reliable total antioxidant capacity (TAC) assay is essential for food safety evaluation and human health monitoring. Herein, a trimetallic nanozyme (FeO@Ag@Pt) was synthesized and exhibited OXD-, POD- and SOD-like activities, which could generate a synergistic catalytic system. FeO@Ag@Pt can catalyze oxygen to produce various reactive oxygen intermediates, and the endogenous product HO could be captured and further dissociated efficiently into •OH, due to its strong substrate binding affinity.

A universal nucleic acid detection platform combing CRISPR/Cas12a and strand displacement amplification with multiple signal readout.

Rapid and sensitive detection of nucleic acids has become crucial in various fields. However, most current nucleic acid detection methods can only be used in specific scenarios, such as RT-qPCR, which relies on fluorometer for signal readout, limiting its application at home or in the field due to its high price. In this paper, a universal nucleic acid detection platform combing CRISPR/Cas12a and strand displacement amplification (CRISPR-SDA) with multiple signal readout was established to adapt to different application scenarios.