Rapid, sensitive, and high-throughput nucleic acid testing (NAT) is crucial for diverse applications in plant breeding and crop protection, including genotyping, transgenic detection, and pathogen diagnosis. However, efficient plant DNA/RNA extraction methods suitable for point-of-care testing remain a significant challenge due to the complex plant cell composition. Here, we present a novel in-situ fixation (ISF) method that eliminates the need for sample grinding, water bath, centrifugation, and pipetting, enabling rapid (6 min) and high-throughput (96 samples) nucleic acid extraction from plant leaves.
View Article and Find Full Text PDF: Patients undergoing neoadjuvant therapy have ample time to engage in exercise prehabilitation. This study aimed to describe the current status, facilitators, and barriers of exercise prehabilitation among this population. : This sequential explanatory mixed-methods evaluation was conducted at a general tertiary hospital in Beijing.
View Article and Find Full Text PDFBackground: Diabetic cystopathy (DCP) is linked to bladder nerve conduction disorders, with diabetes-induced neuropathy impairing nerve signal transmission and causing bladder dysfunction. Myosin 5a, vital for neuronal transport, has been linked to neurological disorders, though its role in DCP remains unclear. The objective of this study was to investigate whether Myosin 5a plays a potential regulatory role in Diabetic Cystopathy.
View Article and Find Full Text PDFBackground And Aims: Diabetic patients are highly susceptible to cardiovascular and renal diseases. As a newly updated comprehensive index for assessing cardiovascular health (CVH), Life's essential 8 (LE8) has the potential to serve as a practical tool for evaluating the risk of diabetic kidney disease (DKD). We are committed to exploring the relationship between LE8 and its subscales with DKD in diabetic patients, aiming to provide preliminary evidence for the formulation of clinical strategies.
View Article and Find Full Text PDFLin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
March 2025
To explore relevant factors to accurately diagnose BPPV in vertigo children. A retrospective study was conducted on the proportion of BPPV in children(<18 years) with vertigo who visited the Hearing and Vertigo Diagnosis and Treatment Center of Tianjin Medical University General Hospital from September 2017 to August 2023. The clinical characteristics of BPPV children, including general demographics, medical history, first visit department, comorbidities, canal involvement, response to treatment, and incidence of recurrence, were analyzed.
View Article and Find Full Text PDFLin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
March 2025
To explore the clinical characteristics, audiological outcomes, and factors influencing the efficacy of pharmacological treatment in patients with sudden hearing loss associated with large vestibular aqueduct syndrome(LVAS). A retrospective analysis was conducted on the clinical data of 77 bilateral LVAS patients(117 ears) hospitalized for sudden hearing loss from January 1, 2009, to December 31, 2023. The inclusion criteria required that patients to be diagnosed according to the Valvassori standard and had received standardized pharmacological treatment.
View Article and Find Full Text PDFLin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
March 2025
To summarize and analyze the clinical characteristics of patients with sudden sensorineural hearing loss(SSHL) accompanying diabetes mellitus, to explore whether diabetes affects the treatment outcomes during hospitalization, and to identify the factors influencing the efficacy of SSHL patients with diabetes. A retrospective analysis was conducted on clinical data from 939 patients with SSHL. The baseline characteristics, and onset conditions of the diabetes group(79 cases) and the non-diabetes group(860 cases) were compared.
View Article and Find Full Text PDFLin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
March 2025
To analyze the hearing outcomes of high-risk children of diabetic mothers, especially in the subtypes of pre-pregnancy diabetes and gestational diabetes, in order to provide some reference for clinical practice. The basic characteristics and hearing levels of children whose mothers had a history of diabetes during pregnancy and underwent audiological diagnosis and evaluation at our hospital's Children's Hearing Diagnosis Center from January 2003 to June 2024 were analyzed. T-tests, Wilcoxon rank-sum tests, and chi-square tests were used for inter-group comparisons, with a significance level set at <0.
View Article and Find Full Text PDFThe prevention and precise treatment of early-stage lung adenocarcinoma (LUAD) characterized by small nodules (stage IA) remains a significant challenge for clinicians, which is due largely to the limited understanding of the oncogenic mechanisms spanning from preneoplasia to invasive adenocarcinoma. Our study highlights the pivotal role of cancer cells exhibiting high expression of centromere protein F (CENPF), driven by TP53 mutations, which become increasingly prevalent during the transition from preneoplasia to invasive LUAD. Biologically, cancer cells (CENPF+) exhibited robust proliferative and stem-like capabilities, thereby propelling the malignant progression of early-stage LUAD.
View Article and Find Full Text PDFIntroduction: Lung adenocarcinoma (LUAD) is the most prevalent subtype of lung cancer. Basal membrane (BM) is important to the invasive processes of LUAD. Our object is to explore hub BM-related genes in LUAD.
View Article and Find Full Text PDFNaunyn Schmiedebergs Arch Pharmacol
February 2025
Randomized controlled trials (RCTs) comparing the treatment outcomes and adverse events of venetoclax-containing regimens with chemoimmunotherapy in chronic lymphocytic leukemia (CLL) are scarce, and the available data are heterogeneous. We carried out a meta-analysis and systematic review to explore the efficacy and safety of these novel venetoclax combination therapies in CLL patients. Five RCTs (11 articles) involving 2481 CLL patients were included.
View Article and Find Full Text PDFBackground: According to the updated guidelines, Bayesian-derived area under the curve estimation is recommended to guide vancomycin dosing. However, the Bayesian dosing software that facilitates this procedure has not been adequately assessed in intensive care unit (ICU) patients. This study evaluated the performance of 3 commonly used Bayesian software programs in predicting vancomycin concentrations in ICU patients before they could be utilized for personalized dosing in this population.
View Article and Find Full Text PDFBackground: Despite numerous studies on medulloblastoma (MB) cell heterogeneity, the spatial characteristics of cellular states remain unclear.
Methods: We analyze single-nucleus and spatial transcriptomes and chromatin accessibility from human MB spanning four subgroups, to identify malignant cell populations and describe the spatial evolutionary trajectories. The spatial CNVs patterns and niches were analyzed to investigate the cellular interactions.
Background: Wilms tumor is among the most common pediatric malignant tumors. Although mA modification influences the structure and function of RNA and participates in tumorigenesis, the relationship between mA methyltransferase TRMT61B gene polymorphisms and Wilms tumor susceptibility is unclear.
Methods: We examined the relationship between TRMT61B gene rs4563180 G > C polymorphism (detected by TaqMan probe method) in 414 children with Wilms tumor and 1199 healthy controls.
Introduction: For patients with breast cancer, the amplification of Human Epidermal Growth Factor 2 (HER2) is closely related to their prognosis and treatment decisions. This study aimed to further improve the accuracy and efficiency of HER2 amplification status detection with a deep learning model, and apply the model to predict the efficacy of neoadjuvant therapy.
Methods: We combined Next-Generation Sequencing (NGS) data and IHC staining images of 606 breast cancer patients and developed a Vision Transformer (ViT) deep learning model to identify the amplification of HER2 through these IHC staining images.
Background: Vaccination is an effective measure to prevent herpes zoster and its related complications. The coverage of herpes zoster vaccination is extremely low in China, and there is a notable lack of research investigating the barriers to promoting the herpes zoster vaccine in China.
Objectives: This study aims to survey the status of herpes zoster (HZ) vaccination and its associated factors among individuals aged 50 years and older, it also seeks to identify the barriers to vaccination and dissemination, thereby providing a scientific foundation for enhancing the vaccination rate of herpes zoster.
Cell Death Discov
February 2025
With the development of public health, female diseases have become the focus of current concern. The unique reproductive anatomy of women leads to the development of gynecological diseases gradually become an important part of the socio-economic burden. Epigenetics plays an irreplaceable role in gynecologic diseases.
View Article and Find Full Text PDFObjectives: Internet use and the results of mental health are related. Numbers of studies presented the association between Internet use and depression, and the middle-aged and elderly adults with multimorbidity are of concern. The study aimed to explore the relationship between Internet use and depression in middle-aged and elderly adults with multimorbidity.
View Article and Find Full Text PDFObjective: The C-reactive protein/Lymphocyte Ratio (CLR) is a novel biomarker whose role in the development of chronic kidney disease (CKD) is not well understood. This study aimed to investigate the correlation between CLR and the prevalence of CKD.
Methods: This cross-sectional study included participants from the US National Health and Nutrition Examination Survey conducted between 1999 and 2010.
Background: The clinical course of high-risk neuroblastoma patients remains suboptimal, and the dynamic and reversible nature of cellular senescence provides an opportunity to develop new therapies.
Objective: This study aims to identify unique markers of cellular senescence in neuroblastoma and to explore their clinical significance.
Methods: The impact of multiple genetic regulatory mechanisms on cellular senescence-associated genes (CSAGs) was first assessed.
Introduction: Pelvic organ prolapse (POP) is a widespread disease in women. Despite its significant prevalence, there is a lack of comprehensive global studies, emphasizing the urgent need for tailored prevention and treatment approaches. This study aims to examine the global burden of POP from 1990 to 2021 and project future trends using the GBD data.
View Article and Find Full Text PDFBackground: Accurate differentiation between benign and malignant pancreatic lesions is critical for effective patient management. This study aimed to develop and validate a novel deep learning network using baseline computed tomography (CT) images to predict the classification of pancreatic lesions.
Methods: This retrospective study included 864 patients (422 men, 442 women) with confirmed histopathological results across three medical centers, forming a training cohort, internal testing cohort, and external validation cohort.
Background: The oxidative balance score (OBS) is a comprehensive concept that includes 16 dietary components and four lifestyle factors to assess an individual's exposure to pro-oxidants and antioxidants. This study aims to explore the relationship between OBS and the risk of chronic kidney disease (CKD).
Methods: This cross-sectional study included nationally representative National Health and Nutrition Examination Survey (NHANES) participants aged 18 and above from 2005 to 2018.