Investigation of mosquito larval habitats and insecticide resistance in an area with a high incidence of mosquito-borne diseases in Jining, Shandong Province.

Background: To investigate mosquito larval habitats and resistance to common insecticides in areas with high incidence rates of mosquito-borne diseases in Jining, Shandong Province, and to provide a scientific basis for the future prevention and control of mosquito-borne diseases and the rational use of insecticides.

Methods And Results: From June to September 2018, mosquito habitat characteristics and species compositions in Jintun town were studied through a cross-sectional survey. Larvae and pupae were collected in different habitats using the standard dipping technique.

Extracellular Vesicle lncRNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 Released From Glioma Stem Cells Modulates the Inflammatory Response of Microglia After Lipopolysaccharide Stimulation Through Regulating miR-129-5p/High Mobility Group Box-1 Protein Axis.

Glioma stem cell (GSC)-derived extracellular vesicles (EVs) can mediate the communication between GSCs and microglia. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) expression in GSCs, EVs, and supernatant was detected by real-time PCR. The direct targeting between MALAT1 and miR-129-5p, miR-129-5p, and HMGB1 were tested with luciferase reporter analysis.

Identification of Alzheimer's disease-associated rare coding variants in the ECE2 gene.

Accumulation of amyloid β protein (Aβ) due to increased generation and/or impaired degradation plays an important role in Alzheimer's disease (AD) pathogenesis. In this report, we describe the identification of rare coding mutations in the endothelin-converting enzyme 2 (ECE2) gene in 1 late-onset AD family, and additional case-control cohort analysis indicates ECE2 variants associated with the risk of developing AD. The 2 mutations (R186C and F751S) located in the peptidase domain in the ECE2 protein were found to severely impair the enzymatic activity of ECE2 in Aβ degradation.

Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1.

Diarrhetic shellfish toxins (DSTs) are marine toxins distributed widely in the world, which pose a major threat to the health of mankind. Dinophysistoxin-1 (DTX-1) has the most potent toxicity in DSTs. However, the current detection methods have ethical problems and technical defects.

Prickly waterlily and rigid hornwort genomes shed light on early angiosperm evolution.

Angiosperms represent one of the most spectacular terrestrial radiations on the planet, but their early diversification and phylogenetic relationships remain uncertain. A key reason for this impasse is the paucity of complete genomes representing early-diverging angiosperms. Here, we present high-quality, chromosomal-level genome assemblies of two aquatic species-prickly waterlily (Euryale ferox; Nymphaeales) and the rigid hornwort (Ceratophyllum demersum; Ceratophyllales)-and expand the genomic representation for key sectors of the angiosperm tree of life.

Identification of expanded repeats in NOTCH2NLC in neurodegenerative dementias.

Recently, the (GGC) repeat expansion in the NOTCH2NLC gene has been identified to be associated with neuronal intranuclear inclusion disease (NIID). Given the clinical overlap of dementia-dominant NIID with neurodegenerative dementia, we therefore hypothesized that the NOTCH2NLC repeat expansion might also contribute to these diseases. In the present study, repeat primed polymerase chain reaction (RP-PCR) and GC-rich PCR were conducted to detect the repeats of NOTCH2NLC in a cohort of 1004 patients with neurodegenerative dementias from mainland China.

[Analysis of pathogenic gene variant in a patient with neonatal Donohue syndrome].

Objective: To explore the genetic basis for a newborn infant suspected with Donohue syndrome.

Methods: Whole exome sequencing (WES) was used to screen potential variants in the child. Suspected variants were validated through Sanger sequencing and real-time PCR.

Solidification of electroplating sludge with alkali-activated fly ash to prepare a non-burnt brick and its risk assessment.

Electroplating sludge (ES), a byproduct of the electroplating industry, is considered as hazardous waste because of the presence of several kinds of toxic heavy metals (HMs, , Cr, Ni, Cu and Zn). The improper treatment of ES has resulted in the contamination of the environment and is ultimately harmful to the living biota. Solidification/stabilization is regarded as a promising technique to deal with hazardous wastes with the use of a geopolymer, an excellent material, in this technique.

Expansion of the phenotypic spectrum of X-linked Charcot-Marie-Tooth (CMT) disease.

Charcot-Marie-Tooth (CMT) disease is the most common hereditary peripheral neuropathy. X-linked Charcot-Marie-Tooth disease in the GJB1 gene is known as CMTX1. We report a 14 years-old young man with walked unstably, bilateral strephenopodia, severe alopecia and paroxysmal bilateral upper limbs tremor without obvious muscle atrophy.

TDP-43 facilitates milk lipid secretion by post-transcriptional regulation of Btn1a1 and Xdh.

Milk lipid secretion is a critical process for the delivery of nutrition and energy from parent to offspring. However, the underlying molecular mechanism is less clear. Here we report that TDP-43, a RNA-binding protein, underwent positive selection in the mammalian lineage.

Spatiotemporal Variation of the Association between Urbanicity and Incident Hypertension among Chinese Adults.

Urbanization is believed to result in a transition towards energy-dense diets, sedentary lifestyles, and a subsequent increase in the burden of hypertension (HTN) and other cardiovascular diseases (CVDs) in developing countries. However, the extent to which this occurs is likely dependent on social contexts. We performed multilevel logistic regression models to examine whether the association between incident HTN and the degree to which a community exhibits urban features varied by region (the Northeast, East Coast, Central, and West) within China and period.

High-Brightness and Color-Tunable FAPbBr Perovskite Nanocrystals 2.0 Enable Ultrapure Green Luminescence for Achieving Recommendation 2020 Displays.

To best catch human eyes in next-generation displays, the updated recommendation 2020 (Rec. 2020) standard has called for ultrapure green emitters to be qualified with a narrow emission of 525-535 nm with a full width at half-maximum (fwhm) below 25 nm. However, it is still challenging to find an emitter which can simultaneously cover these two criteria.

Synergism of citric acid and zero-valent iron on Cr(VI) removal from real contaminated soil by electrokinetic remediation.

This study focused on enhanced electrokinetic remediation of Cr(VI) in real contaminated soil. The citric acid (CA) as the electrolyte and Fe(II) released from zero-valent iron (ZVI) under anoxic conditions functioned as the main reducer. They were used for overcoming the high insoluble Cr(VI) fraction in real contaminated soil and high Cr(VI) residue in acidic soil near the anode simultaneously.

Flexible and Transparent Ferroferric Oxide-Modified Silver Nanowire Film for Efficient Electromagnetic Interference Shielding.

Transparent and flexible electromagnetic interference (EMI) shielding film is highly desirable due to the fast-growing flexible electronics. A silver nanowire (Ag NW) film is considered to be an ideal candidate for a transparent and flexible EMI shielding film but suffers low EMI shielding effectiveness (SE) at high transparency and poor bending durability. Herein, we introduce ferroferric oxide (FeO) into a Ag NW film and demonstrate a robust EMI shielding film, which exhibits SE of 24.

Colorimetric aptasensor for ochratoxin A detection based on enzyme-induced gold nanoparticle aggregation.

An innovative colorimetric method based on enzyme-induced gold nanoparticle aggregation was developed to detect the activity of alkaline phosphatase (ALP), and it was further applied to construct an aptasensor to monitor ochratoxin A (OTA) concentrations. In the presence of ALP, the substrate ascorbic acid 2-phosphate was hydrolyzed to generate ascorbic acid (AA). Subsequently, reduction of MnO nanosheets by AA produced manganese ions, which mediated gold nanoparticle aggregation.

A recombinase polymerase amplification-lateral flow dipstick assay for rapid detection of the quarantine citrus pathogen in China, .

, the causal agent of brown rot of citrus fruit, is an important worldwide pathogen and a quarantine pest in China. Current diagnosis of the disease relies on disease symptoms, pathogen isolation and identification by DNA sequencing. However, symptoms caused by can be confused with those by other and fungal species.

Marine Actinomycetes-derived Natural Products.

Actinomycetes is an abundant resource for discovering a large number of lead compounds, which play an important role in microbial drug discovery. Compared to terrestrial microorganisms, marine actinomycetes have unique metabolic pathways because of their special living environment, which has the potential to produce a variety of bioactive substances. In this paper, secondary metabolites isolated from marine actinomycetes are reviewed (2013-2018), most of which exhibited cytotoxic, antibacterial, and antiviral biological activities.

Primary low grade myxofibrosarcoma of the liver with benign presentation but malignant outcome: a case report.

Background: Myxofibrosarcoma (MFS) is most often found on the limbs of aged male people, but extremely uncommon in the liver.

Case Presentation: A 52-year-old female patient with a liver mass was diagnosed as a primary MFS. It had no obvious abdominal symptoms, and the tumor was resected with an extended margin.

Eutypellacytosporins A-D, Meroterpenoids from the Arctic Fungus sp. D-1.

The Arctic fungus sp. D-1, previously found to produce a variety of cytotoxic cyclopropyl-fused and cyclobutyl-fused pimarane diterpenoids when grown in the defined medium, was induced to produce unusual metabolites by growing on solid rice medium. A chemical investigation on the rice medium extract led to the isolation of four new meroterpenoids, eutypellacytosporins A-D (-), along with the known biogenetically related compound cytosporin D ().

Study of the binding mechanism of aptamer to palytoxin by docking and molecular simulation.

This paper provides a feasible model for molecular structure analysis and interaction mechanism of aptamer and micromolecule. In this study, modeling and dynamic simulation of ssDNA aptamer (P-18S2) and target (Palytoxin, PTX) were performed separately. Then, the complex structure between DNA and PTX was predicted, and docking results showed that PTX could combine steadily at the groove's top of DNA model by strong hydrogen-bonds and electrostatic interaction.

Establishment of a reference procedure to measure urine-formed elements and evaluation of an automated urine analyzer.

A standardized reference method is needed to accurately and precisely measure urine-formed elements (UFEs; red blood cells [RBCs], white blood cells [WBCs], and squamous epithelial cells [sECs]). We compared the results from a standard method with those from an automated analyzer. Trained technicians used standardized bright-field microscopy of fresh non-centrifuged urine samples, and disposable 1 µl chambers.

Mutation analysis of GLT8D1 and ARPP21 genes in amyotrophic lateral sclerosis patients from mainland China.

Variants in exon 4 of gene encoding GLT8D1 (glycosyltransferase 8 domain containing 1) gene have recently been suggested as a novel cause of amyotrophic lateral sclerosis (ALS). In addition, there is a synergism between GLT8D1 and ARPP21 (cAMP Regulated Phosphoprotein 21) variants for ALS. However, this observation has not been validated in other ALS cohorts.

QiDiTangShen Granules Activate Renal Nutrient-Sensing Associated Autophagy in db/db Mice.

QiDiTangShen granules (QDTS) have been proven to reduce the proteinuria in patients with diabetic nephropathy (DN) effectively. The present study was aimed to investigate the mechanism underlying QDTS's renoprotection. The main components of QDTS were identified by ultra-high liquid chromatography-tandem mass spectrometry and pharmacological databases, among which active components were screened by oral bioavailability and drug-likeness.

Synthesis, Purification, and Selective β-AR Agonist and Bronchodilatory Effects of Catecholic Tetrahydroisoquinolines from .

A green, biomimetic, phosphate-mediated Pictet-Spengler reaction was used in the synthesis of three catecholic tetrahydroisoquinolines, , , and , present in the medicinal plant , as well as their analogues -, , and , with dopamine hydrochloride and aldehydes as the substrates. AB-8 macroporous resin column chromatography was applied for purification of the products from the one-step high-efficacy synthesis. It eliminated the difficulties in the isolation of catecholic tetrahydroisoquinolines from the aqueous reaction system and unreacted dopamine hydrochloride.