Genome-wide identification and expression analysis of GDP-D-mannose pyrophosphorylase and KATANIN in .

The GDP-D-mannose pyrophosphorylase () and microtubule severing enzyme KATANIN () are crucial for wood formation. Although functional identification has been performed in , few comprehensive studies have been conducted in forest trees. In this study, we discovered 8 and 4 genes by analyzing the whole genome sequence of .

Population structure and genetic diversity in based on SNP markers.

has the characteristics of rapid growth and high resistance. However, there is little research on molecular breeding of , which is essential to shortening breeding life and selecting quality varieties. Therefore, a crucial step before selective breeding can be carried out to increase the wood quality of is identifying genetic diversity and population structure using single nucleotide polymorphism (SNP) markers.

Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages.

Background: Wood is a secondary xylem generated by vascular cambium. Vascular cambium activities mainly include cambium proliferation and vascular tissue formation through secondary growth, thereby producing new secondary phloem inward and secondary xylem outward and leading to continuous tree thickening and wood formation. Wood formation is a complex biological process, which is strictly regulated by multiple genes.

Regeneration and Agrobacterium-mediated genetic transformation of twelve species.

is a genus of over 900 species and hybrids, and many of them are valuable fast-growing hardwoods. Due to its economic importance, is one of the early tree species whose genomes were deciphered. However, the lack of efficient genetic transformation systems severely restricts the functional genomic research on the plant.

High-throughput "read-on-ski" automated imaging and label-free detection system for toxicity screening of compounds using personalised human kidney organoids.

Organoid models are used to study kidney physiology, such as the assessment of nephrotoxicity and underlying disease processes. Personalized human pluripotent stem cell-derived kidney organoids are ideal models for compound toxicity studies, but there is a need to accelerate basic and translational research in the field. Here, we developed an automated continuous imaging setup with the "read-on-ski" law of control to maximize temporal resolution with minimum culture plate vibration.

Genomic Studies Reveal Substantial Dominant Effects and Improved Genomic Predictions in an Open-Pollinated Breeding Population of .

Most of the genomic studies in plants and animals have used additive models for studying genetic parameters and prediction accuracies. In this study, we used genomic models with additive and nonadditive effects to analyze the genetic architecture of growth and wood traits in an open-pollinated (OP) population of We used two progeny trials consisting of 5742 trees from 244 OP families to estimate genetic parameters and to test genomic prediction accuracies of three growth traits (diameter at breast height - DBH, total height - Ht and tree volume - Vol) and kraft pulp yield (KPY). From 5742 trees, 468 trees from 28 families were genotyped with 2023 pre-selected markers from candidate genes.

Studies on a new stainless steel mesh dynamic membrane for wastewater treatment.

In this study an innovative dynamic membrane bioreactor (DMBR) was implemented from a stainless-steel mesh filter, which was used as a support material, and activated sludge used as an adsorption and filtration interface containing particulate organics. This work indicated that DMBR can achieve rapid solid-liquid pollutants separation. The activated sludge in the aeration tank quickly formed a thin dynamic membrane layer on the filter.

The first identification of genomic loci in plants associated with resistance to galling insects: a case study in Eucalyptus L'Hér. (Myrtaceae).

Genomic loci related with resistance to gall-inducing insects have not been identified in any plants. Here, association mapping was used to identify molecular markers for resistance to the gall wasp Leptocybe invasa in two Eucalyptus species. A total of 86 simple sequence repeats (SSR) markers were screened out from 839 SSRs and used for association mapping in E.

[Rapid enrichment and cultivation of denitrifying phosphate-removal bacteria and its identification by fluorescence in situ hybridization technology].

The present work focused on a rapid enrichment and cultivation of denitrifying phosphate-removal bacteria (DPB) in a membrane bio-reactor(MBR) by using A2/O anaerobic sludge from a wastewater treatment plant as seed, as well as providing an identification method. In the experiments, sodium acetate was used as the carbon source and a certain amount of nitrate was added to the MBR in the anoxic stage. Results showed that, with the efficient trap of the hollow-fiber membrane module, the proportion of DPB in all the phosphate-accumulating organisms (PAOs) increased from 24% to 93% within 35 days after two-stage's cultivation including anaerobic/aerobic and anaerobic/anoxic, during which the removal efficiency of nitrogen and phosphorus reached more than 90%.

[Synergistic effects of nano-sized magnetic particles and uncoupler to the characteristics of activated sludge].

For improving the performance and sludge settling property of an activated sludge reduction process with uncoupler, in this investigation, uncoupler and nano-sized magnetic particles were added simultaneously to a sequencing batch reactor for exploring their synergistic effects to the characteristics of activated sludge. The results showed that the volume reduction of sludge reached 41% with single 2,4,5-Trichlorophenol (TCP) Comparing with the control experiment, the biodegradability and settling properties of the activated sludge decreased. Under the actions of TCP combined with nano-sized magnetic particles, the volume reduction of sludge reached 34%, the removal efficiencies of COD, nitrogen, and phosphorus as well as the sludge settling property were not significantly influenced.

Energy efficiency of pre-treating excess sewage sludge with microwave irradiation.

The objective of this study is to investigate the energy consumption of pre-treating excess sewage sludge with microwave irradiation using several parameters, including temperature rise, degree of cell destruction, SCOD/TCOD ratio (solids solubilization), and biogas production to evaluate the energy efficiency. It was found that water content was the most important factor that influenced the energy efficiency of raising the temperature and promoting the solubilization of solid materials. Increasing specific energy (E(s)) accelerated the biogas production, but there was a limit to this process.