Integrated analyses of characterization and transcriptome reveal the adaptive response mechanism of Bacillus cereus FCHN 7-1 in cadmium adsorption.

Cadmium (Cd) pollution is a pressing environmental issue that seriously threatens human health. In recent years, microbial extracellular polymeric substances (EPS) have been developed as an eco-friendly and effective solution for heavy metal bioremediation. In this study, Bacillus cereus FCHN 7-1, which was isolated from highly Cd-polluted soils in Hunan Province, China, has strong resistance to Cd and excellent Cd adsorptive capacity.

Replacing traditional coffee appraisers with inductively coupled plasma - mass spectrometry (ICP-MS): From manual sensory evaluation to scientific analysis.

Scientific development provides opportunities to replace many traditional manual methods to achieve more accurate results and higher efficiency. To scientifically ascertain the geographical origin of coffee, this study develops a method for the rapid determination of 16 inorganic elements in coffee using microwave digestion combined with the ICP-MS internal standard method. Principal component analysis (PCA), Fisher discriminant analysis (FDA), and Partial least squares discriminant analysis (PLS-DA) are employed to analyze 40 coffee samples from three production areas of Pu'er, Baoshan, and Wanning.

Mangrove fungi in action: Novel bioremediation strategy for high-chloride wastewater.

Bioremediation of extremely high-chloride wastewater poses significant challenges due to the adverse effects of elevated salt concentrations on most microorganisms, where chloride levels can be as high as 7% (w/v). Mangrove wetlands derived fungus, Aspergillus aculeatus, emerged as a promising candidate, capable of removing approximately 40% of chloride ions in environments with concentration of 15% (w/v), representative of industrial wastewater conditions. Transcriptomics and biochemical assays conducted under increasing salt conditions revealed that elevated chloride concentrations induce the expression and activity of S-adenosyl methionine-dependent methyltransferase, which facilitates the conversion of chloride into chloromethane.

ZIF-67-Derived Co/N-Doped Carbon-Functionalized MXene for Enhanced Electrochemical Sensing of Carbendazim.

Herein, ZIF-67-derived Co and N-doped carbon (Co/NC) particle-modified multilayer MXene (MXene@Co/NC) was developed as remarkable electrode material for carbendazim (CBZ) detection. MXene as a substrate provides an excellent conductive framework and plentiful accessibility sites. Co/NC particles embedding in MXene can not only prevent the interlayer stacking of MXene but also contribute a great deal of metal catalytic active sites and finally improve the adsorption and catalytic properties of the composite.

Responses of soil microbial communities to concentration gradients of antibiotic residues in typical greenhouse vegetable soils.

To explore the responses of soil microbial communities to concentration gradients of antibiotic residues in soil, 32 soil samples were collected from a typical greenhouse vegetable production base in Northern China in 2019. The total concentrations of 26 antibiotic residues in these soil samples was 83.24-4237.

Florfenicol alleviated lipopolysaccharide (LPS)-induced inflammatory responses in Ctenopharyngodon idella through inhibiting toll / NF-κB signaling pathways.

The present study was conducted to evaluate the anti-inflammatory activity of florfenicol (FFC) against lipopolysaccharide (LPS)-induced inflammatory responses in Ctenopharyngodon idella in vivo and in vitro. Head-kidney (HK) macrophages were pre-treated with 10 μg/mL LPS and then exposed to different concentrations of FFC to determine its in vitro anti-inflammatory activity. Inhibitory effect of FFC on inflammatory mediators TNF-α, IL-6 and IL-1β, as well as LPS-induced nitric oxide (NO) and prostaglandin E 2 (PGE 2) production were assayed by ELISA.

P,N Codoped carbon dots as an efficient "off-on" fluorescent probe for lipoic acid detection and its cellular dual-color imaging.

A facile single hydrothermal method was developed to synthetize P,N codoped carbon dots (P,N/CDs), which show strong and stable fluorescence, good water solubility, low toxicity and good biocompatibility. Hence, a novel and efficient "off-on" P,N/CDs fluorescent probe was developed for the highly sensitive detection of lipoic acid (LA) for the first time. The fluorescence of the P,N/CDs was quenched by Cu forming a P,N/CDs-Cu complex, which acted as the "off" process, but Cu could be removed by LA, due to stronger chelating between LA and Cu, forming a more stable complex, which recovered the fluorescence of the P,N/CDs, in order to achieve the "on" process.