Enhancing Mushroom Yield and Quality Through the Amendment of Soil Physicochemical Properties and Microbial Community with Wood Ash.

mushroom is a nutritionally rich and rare edible fungus. The traditional cultivation model, which relies on expanding the cultivation area to meet market demand, is no longer sufficient to address the rapidly growing market demand. Enhancing the yield and quality of without increasing the cultivation area is an intractable challenge in the development of the mushroom industry.

Systematic analysis of natural topoisomerase I inhibitors from by ultrafiltration-UPLC-ESI-MS/MS, pharmacophore modelling, and molecular dynamics simulation.

This study conducted a systematic analysis to explore natural DNA topoisomerase I (topo I) inhibitors from (). Crude extract of exhibited notable toxic and anti-proliferative effects on A549 cells. A total of 36 components were identified using bioaffinity ultrafiltration UPLC-ESI-MS/MS.

Chemical structures, biological activities, and biosynthetic analysis of secondary metabolites of the Diatrypaceae family: A comprehensive review.

The family Diatrypaceae is a less well-known group within the order Xylariales (Ascomycota). Initially, the focus on its metabolites was related to the pathogenicity of one of its members, . To date, a total of 254 natural products have been identified from Diatrypaceae strains.

The chemical structures, biosynthesis, and biological activities of secondary metabolites from the culinary-medicinal mushrooms of the genus Hericium: a review.

Fungal phytochemicals derived from higher fungi, particularly those from the culinary-medicinal genus Hericium, have gained significant attention in drug discovery and healthcare. This review aims to provide a comprehensive analysis of the chemical structures, biosynthetic pathways, biological activities, and pharmacological properties of monomeric compounds isolated from Hericium species. Over the past 34 years, 253 metabolites have been identified from various Hericium species, including cyathane diterpenes, alkaloids, benzofurans, chromenes, phenols, pyrones, steroids, and other miscellaneous compounds.

Evaluation of Phenazine Derivatives from the Lichen-Associated as Potent Antineuroinflammatory Agents In Vitro and In Vivo.

Eighteen nitrogen-containing compounds (-) were isolated from cultures of the lichen-associated collected from the Qinghai-Tibet Plateau, including seven phenazine derivatives with three new ones, named subphenazines A-C (-), two new furan pyrrolidones (-), and nine known alkaloids. The structures were elucidated by spectroscopic data analysis, and absolute configurations were determined by single-crystal X-ray diffraction and ECD calculations. The phenazine-type derivatives, in particular compound , exhibited significantly better antineuroinflammatory activity than other isolated compounds (-).

Genome-wide characterization and metabolite profiling of Cyathus olla: insights into the biosynthesis of medicinal compounds.

Cyathus olla, belonging to the genus Cyathus within the order Agaricales, is renowned for its bird's nest-like fruiting bodies and has been utilized in folk medicine. However, its genome remains poorly understood. To investigate genomic diversity within the genus Cyathus and elucidate biosynthetic pathways for medicinal compounds, we generated a high-quality genome assembly of C.

Evaluation of Quality in 15 Production Areas Using Metabolomics and the Membership Function Method.

is a precious medicinal and edible fungus, which is widely used in body health care and disease prevention. The current research focuses on the comparison of metabolite characteristics between a small number of samples and lacks a comprehensive evaluation of the quality of in a large-scale space. In this study, LC-MS/MS, principal component analysis (PCA), hierarchical cluster analysis (HCA), and the membership function method were used to comprehensively evaluate the characteristics and quality of metabolites in 15 main producing areas of in China.

Chemical Structures, Biological Activities, and Biosynthetic Analysis of Secondary Metabolites from Mushrooms: A Review.

mushrooms are an important genus in the Agaricaceae family, belonging to the order Agaricales of the class Basidiomycota. Among them, is a common mushroom for mass consumption, which is not only nutritious but also possesses significant medicinal properties such as anticancer, antibacterial, antioxidant, and immunomodulatory properties. The rare edible mushroom, , contains unique agaricol compounds with significant anticancer activity against liver cancer.

Functional Characterization of Sesquiterpene Synthases and P450 Enzymes in for Biosynthesis of Spiro [4.5] Decane Terpene.

, a popular edible mushroom, contains sesquiterpenes with potential health benefits. We characterized 12 sesquiterpene synthases and one P450 enzyme in using as a heterologous expression system, culminating in the biosynthesis of 16 distinct sesquiterpene compounds. An enzyme encoded by the gene responsible for the synthesis of the spiro [4.

Biochemical characterization of a multiple prenyltransferase from Tolypocladium inflatum.

Prenylation plays a pivotal role in the diversification and biological activities of natural products. This study presents the functional characterization of TolF, a multiple prenyltransferase from Tolypocladium inflatum. The heterologous expression of tolF in Aspergillus oryzae, coupled with feeding the transformed strain with paxilline, resulted in the production of 20- and 22-prenylpaxilline.

Natural and engineered xylosyl products from microbial source.

Glycosylation is a prevalent post-modification found in natural products and has a significant impact on the structural diversity and activity variation of natural products. Glucosylation is the most common type of glycosylation, whereas xylosylation is relatively rare. Despite their unique chemical structures and beneficial activities, xylosylated natural products from microorganisms have received little attention.

Effect of Air Drying on the Metabolic Profile of Fresh Wild and Artificial .

Fresh and dried are widely used by the public for medicinal and health purposes. However, the differences between them have not been examined. In this study, fresh wild and artificial (WFC and AFC) were dried to obtain dried wild and artificial (WDC and ADC).

The alkynyl-containing compounds from mushrooms and their biological activities.

Mushrooms have been utilized by humans for thousands of years due to their medicinal and nutritional properties. They are a crucial natural source of bioactive secondary metabolites, and recent advancements have led to the isolation of several alkynyl-containing compounds with potential medicinal uses. Despite their relatively low abundance, naturally occurring alkynyl compounds have attracted considerable attention due to their high reactivity.

Comparative Genomic Analysis and Metabolic Potential Profiling of a Novel Culinary-Medicinal Mushroom, (Basidiomycota).

is an emerging species in the genus with few members. Despite being highly regarded due to its rarity, knowledge about remains limited. In this study, we sequenced, de novo assembled, and annotated the complete genome of NPCB A08, isolated from the Qinling Mountains in Shaanxi, China, using the Illumina NovaSeq and Nanopore PromethION technologies.

Molecular properties, structure, neurotrophic and anti-inflammatory activities of cultured secondary metabolites from the cultures of the mushroom CBPFE A06.

Two previously undescribed natural cyathane diterpenoids Me-dentifragilin A () and Epi-neocyathin O (), and three known cyathane diterpenoids -, cyathin O, neocyathin P, and cyathin I, were isolated from the rice medium of the CBPFE A06. Their structures were established by NMR spectra, and HR-ESI-MS. Compounds - displayed encouraging neurotrophic activity in PC-12 cells at doses of 5 µM.

Combination of bioaffinity ultrafiltration-UFLC-ESI-Q/TOF-MS/MS, in silico docking and multiple complex networks to explore antitumor mechanism of topoisomerase I inhibitors from Artemisiae Scopariae Herba.

Background: Artemisiae Scopariae Herba (ASH) has been widely used as plant medicine in East Asia with remarkable antitumor activity. However, the underlying mechanisms have not been fully elucidated.

Methods: This study aimed to construct a multi-disciplinary approach to screen topoisomerase I (topo I) inhibitors from ASH extract, and explore the antitumor mechanisms.

Unprecedented Neoverrucosane and Cyathane Diterpenoids with Anti-Neuroinflammatory Activity from Cultures of the Culinary-Medicinal Mushroom .

The culinary medicinal mushroom holds significant global esteem and has garnered heightened interest within increasingly ageing societies due to its pronounced neuroprotective and anti-neuroinflammatory properties. Within this study, two novel diterpenes, 16-carboxy-13--neoverrucosane () and Erinacine L (); three known xylosyl cyathane diterpenoids, Erinacine A (), Erinacine C (), and Erinacine F (); and four lanostane-type triterpenoids, and three cyclic dipeptides (-), in addition to orcinol (), were isolated from the rice-based cultivation medium of . Their structures were determined by NMR, HR-ESI-MS, ECD, and calculated NMR.

Bioinformatic Analysis of Secondary Metabolite Biosynthetic Potential in Pathogenic .

species are among the filamentous fungi with the most pronounced impact on agricultural production and human health. The mycotoxins produced by pathogenic not only attack various plants including crops, causing various plant diseases that lead to reduced yields and even death, but also penetrate into the food chain of humans and animals to cause food poisoning and consequent health hazards. Although sporadic studies have revealed some of the biosynthetic pathways of toxins, they are insufficient to satisfy the need for a comprehensive understanding of toxin production.

The Complete Genomic Sequence of Microbial Transglutaminase Producer, Streptomyces mobaraensis DSM40587.

Actinomycetes are remarkable natural sources of active natural molecules and enzymes of considerable industrial value. Streptomyces mobaraensis is the first microorganism found to produce transglutaminase with broad industrial applications. Although transglutaminase in S.

The First Whole Genome Sequencing of and Its Metabolite Profiling.

, an emerging wild mushroom with remarkable biological activities and a distinctive oversized mushroom shape, has gained increasing attention in recent years. Despite its status as an important resource of wild edible fungi, knowledge about this mushroom is still limited. In this study, we used the Illumina NovaSeq and Nanopore PromethION platforms to sequence, de novo assemble, and annotate the whole genome and mitochondrial genome (mitogenome) of the strain BH01 isolated from Bosten Lake, Xinjiang Province, China.

Secondary Metabolites of Bird's Nest Fungi: Chemical Structures and Biological Activities.

Bird's nest fungi, a general term for species in the family Nidulariaceae, are named for their fruiting bodies that resemble bird's nests. Two of their members, (Schw.) de Toni.