The Effect of Cryopreservation on T-Cell Subsets by Flow Cytometry Automated Algorithmic Analysis and Conventional Analysis.

Background: Low-temperature cryopreservation is a common method for scientific research and clinical sample preservation when utilizing flow cytometry. In flow cytometry data analysis, traditional manual "gating" is susceptible to past experience and faces the challenge of manual subjective bias, time-consuming, and multidimensional data analysis. With the development of algorithms, the advantages of dimensionality reduction and clustering in result analysis are gradually becoming more prominent.

Effects of Five Different Withering Methods on the Composition and Quality of Congou Black Tea.

To explore the effects of different withering methods on the quality of Congou black tea, this study focused on five different withering methods: natural withering, warm-air withering, sun-natural combined withering, sun withering, and shaking withering. Gas chromatography‒mass spectrometry (GC‒MS), high-performance liquid chromatography (HPLC), and ion-exchange chromatography techniques were used to analyze the nonvolatile and volatile components and composition of the tea. The results revealed significant differences ( < 0.

Microplastics occurrence and distribution characteristics in mulched agricultural soils of Guizhou province.

Microplastics pollution in agricultural soil is increasingly recognized, but the specific situation varies with geography, climate conditions, and farming practices. The karst landscape, a typical geomorphology in China, demands a deeper understanding of microplastics pollution in such areas. This research zeroes in on Guizhou, a province known for its karst formations, by collecting soil samples from the mulched cultivation layer in ten counties and cities.

Origin and Function of Monocytes in Inflammatory Bowel Disease.

Inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), is a chronic disease resulting from the interaction of various factors such as social elements, autoimmunity, genetics, and gut microbiota. Alarmingly, recent epidemiological data points to a surging incidence of IBD, underscoring an urgent imperative: to delineate the intricate mechanisms driving its onset. Such insights are paramount, not only for enhancing our comprehension of IBD pathogenesis but also for refining diagnostic and therapeutic paradigms.

Function of homocysteine and HE4 in endometrial carcinoma: verified by prospective experiment.

Background: Timely diagnosis is the key factor to improve the prognosis of endometrial carcinoma (EC). To date, no particularly good markers could significantly improve the detection rate of EC. This study aimed to assess the utility of serum markers homocysteine (Hcy), human epididymal protein 4 (HE4), cancer antigen 199 (CA199), cancer antigen 125 (CA125), fibrinogen (Fib), and D-dimer (D-D) for EC diagnosis, especially Hcy of which its role in EC has not been noticed.

Integrated transcriptomic and proteomic analyses reveal the molecular mechanism underlying the thermotolerant response of Spodoptera frugiperda.

Spodoptera frugiperda (Lepidoptera: Noctuidae) is a highly destructive invasive pest with remarkable adaptability to extreme climatic conditions, posing a substantial global threat. Although the effects of temperature stress on the biological and ecological properties of S. frugiperda have been elucidated, the molecular mechanisms underlying its responses remain unclear.

The gut microbiota participates in the effect of linaclotide in patients with irritable bowel syndrome with constipation (IBS-C): a multicenter, prospective, pre-post study.

Background: Interindividual variation characterizes the relief experienced by constipation-predominant irritable bowel syndrome (IBS-C) patients following linaclotide treatment. Complex bidirectional interactions occur between the gut microbiota and various clinical drugs. To date, no established evidence has elucidated the interactions between the gut microbiota and linaclotide.

Ecological stoichiometry of leaf-litter-fine roots in mixed plantations in mountainous area of Southern Ningxia, China.

The southern mountainous areas in Ningxia are representative regions of the Loess Plateau, with extremely fragile ecological environment. Large area of pure plantations established during the project of Grain for Green has suffered from poor nutrient availability and biodiversity loss, while planting mixed plantations is commonly consi-dered as an effective way to improve the ecological benefits. We selected + mixed plantation, + mixed plantation, pure plantation and pure plantation located ina Ningnan mountainous area as test objects.

The Clinicopathologic and Prognostic Significance of c-Myc Expression in Hepatocellular Carcinoma: A Meta-Analysis.

The incidence and mortality rates of hepatocellular carcinoma (HCC) are increasing worldwide. Therefore, there is an urgent need to elucidate the molecular drivers of HCC for potential early diagnosis and individualized treatment. Whether c-Myc expression plays a role in the clinicopathology and prognosis of patients with HCC remains controversial.

Development and Validation of an Artificial Intelligence Model for Small Bowel Capsule Endoscopy Video Review.

Importance: Reading small bowel capsule endoscopy (SBCE) videos is a tedious task for clinicians, and a new method should be applied to solve the situation.

Objectives: To develop and evaluate the performance of a convolutional neural network algorithm for SBCE video review in real-life clinical care.

Design, Setting, And Participants: In this multicenter, retrospective diagnostic study, a deep learning neural network (SmartScan) was trained and validated for the SBCE video review.

Characterization of the complete chloroplast genome of the invasive plant (L.) Pers. (Asterales: Asteraceae).

(L.) Pers. (annual, daisy or tall fleabane) is an annual herb native to North America but has been introduced and naturalized worldwide.

A Clinical Study on Individualized Surgical Scheme of Hydrocephalus Complicated With Skull Defect.

Objective: This study intends to discuss the individualized selection of surgical scheme of hydrocephalus complicated with skull defect.

Methods: A total of 141 patients with hydrocephalus complicated with skull defect in our hospital from January 2012 to December 2018 were the main subjects of this study. Among these 141 patients, 78 patients underwent shunt and skull repair surgery in our hospital.

Endoscopic-assisted surgery for skull defects with subdural effusion.

Introduction: Subdural effusion is a common complication that occurs after decompressive craniectomy. According to the endoscopy results, the formation mechanism of subdural effusion after decompressive craniectomy was discussed.

Aim: The morphological structure of subdural effusion in skull defects was observed with endoscopy, and endoscopic-assisted surgery was performed for subdural effusion.

Nicotinamide Overcomes Doxorubicin Resistance of Breast Cancer Cells through Deregulating SIRT1/Akt Pathway.

Background And Purpose: Breast cancer is one of the leading causes of cancer deaths in female worldwide. Doxorubicin represents the most common chemotherapy for breast cancer, whereas side effects and development of resistance impede its effect on chemotherapy. Nicotinamide (NAM), serves as the sirtuins' inhibitor, effectively suppressing various types of cancer.

AKT phosphorylation sites of Ser473 and Thr308 regulate AKT degradation.

Protein kinase B (AKT) is a serine-threonine kinase that mediates diverse cellular processes in a variety of human diseases. Phosphorylation is always the best studied posttranslational modification of AKT and a connection between phosphorylation and ubiquitination has been explored recently. Ubiquitination of AKT is an important step for its phosphorylation and activation, while whether phosphorylated AKT regulated its ubiquitination status is still unknow.

Investigating the application of diving endoscopic technique in determining the extent of pituitary adenoma resection via the trans-nasal-sphenoidal approach.

The aim of the present study was to investigate the advantages and disadvantages of the diving endoscopic technique in pituitary adenoma surgery, and the application value in determining the extent of tumor resection. A total of 37 patients with pituitary adenoma initially underwent tumor resection under an endoscope-assisted microscope via standard trans-nasal-sphenoidal approach, and tumor cavity structure was observed by applying the diving endoscopic technique. Surgery was subsequently performed again under a microscope or endoscope.

Diagnostic value of fibulin-3 for malignant pleural mesothelioma: A systematic review and meta-analysis.

Background: Several studies have investigated the diagnostic value of fibulin-3 for malignant pleural mesothelioma (MPM), but the results were various. Therefore, we performed a systematic review and meta-analysis to evaluate the diagnostic value of fibulin-3 for MPM.

Results: Eight studies were included in this work.

Neuroprotective effect of berberine is mediated by MAPK signaling pathway in experimental diabetic neuropathy in rats.

The mechanisms leading to diabetic neuropathy are complex. As an active component in several traditional Chinese medicines, berberine has a beneficial effect in the treatment of diabetes with hyperlipidemia. This study evaluated the protective effects of berberine on diabetic neuropathy induced by streptozotocin and a high-carbohydrate/high-fat diet in rats.

Long non-coding RNAs in colorectal cancer.

Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. Despite substantial progress in understanding the molecular mechanisms and treatment of CRC in recent years, the overall survival rate of CRC patients has not improved dramatically. The development of CRC is multifactor and multistep processes, in which abnormal gene expression may play an important role.

[Regeneration mode of Xanthoceras sorbifolia population and its contribution to population regeneration].

Sexual and asexual propagation of Xanthoceras sorbifolia natural populations in different habitats and their importance in population regeneration were studied. The results showed that X. sorbifolia populations were capable of the sexual and asexual propagation in 3 slope aspects, but a balance between the two propagation modes was observed.

CRH knockout inhibits the murine innate immune responses in association with endoplasmic reticulum stress after thermal injury.

Background: Previous studies in our laboratory have demonstrated the hypothalamus destruction and adrenalectomy could blunt the innate immunity while boosting the excessive inflammation after injury. We aimed to investigate the effects of corticotrophin-releasing hormone knockout (CRH KO) on the innate immune responses in macrophages as well as to elucidate the underlying mechanism.

Methods: The chemotaxis and phagocytosis activities of macrophages, bacteria translocation, plasma tumor necrosis factor (TNF)-α secretion, and intestinal injury were observed in the presence of the endoplasmic reticulum stress after thermal injury in CRH KO mice.

Epinephrine enhances the response of macrophages under LPS stimulation.

Trauma associated with infection may directly trigger a neuroendocrine reaction in vivo while the hormone epinephrine is known to mediate immune responses to inflammation after injury. However, the role of epinephrine during the earliest stage of trauma still remains unclear. We therefore explored the role of epinephrine on activated macrophages under LPS stimulation in vitro as well as the mechanisms underlying its effect.

[Effects of thinning intensities on population regeneration of natural Quercus variabilis forest on the south slope of Qinling Mountains].

Taking the natural Quercus variabilis forest in Shangluo, south slope of Qinling Mountains as the object in May 2006 and August 2011, which was under close-to-natural management of different thinning intensities (30%, 20%, 10%), and the un-thinned forest as the control, changes of the stand growth situation before and after thinning, population regeneration, species diversity and soil fertility after 5 years of thinning were analyzed, and the effects of thinning on forestland revegetation and community development were evaluated comprehensively. The results showed that the number of 1-6 years old Q. variabilis seedlings increased with increasing thinning intensity, while no significant difference was found for above 6 years old seedlings.

[Seedling regeneration and affecting factors of Quercus variabilis in different distribution regions].

Twenty four fixed plots in three distribution regions of Quercus variabilis (Loess Plateau, marginal distribution zone; north slope of Qinling Mountains, semi-arid core area; and south slope of Qinling Mountains, moist core area) were installed, respectively, to investigate the age structure, growth status, and dry mass accumulation and allocation of 1-8 years old Q. variabilis seedlings, and path analysis was adopted to determine the key factors affecting the regeneration of the seedlings. In the distribution regions, the density of the seedlings decreased with their increasing age, and the density of the 1-8 years old seedlings all decreased in the order of south slope of Qin-ling Mountains > north slope of Qinling Mountains > Loess Plateau.