Genomic characterization and outbreak investigations of methicillin-resistant in a county-level hospital in China.

Methicillin-resistant (MRSA) is a common pathogen contributing to healthcare-associated infections, which can result in multiple sites infections. The epidemiological characteristics of MRSA exhibit variability among distinct regions and healthcare facilities. The aim of this study was to investigate the molecular epidemiology and nosocomial outbreak characteristics of MRSA in a county-level hospital in China.

Adipose-derived Mesenchymal Stem Cells are Ideal for the Cell-based Treatment of Refractory Wounds: Strong Potential for Angiogenesis.

Although Mesenchymal Stem Cells (MSCs)-based therapy has been proposed as a promising strategy for the treatment of chronic lower-extremity ulcers, their optimal sources, amounts, and delivery methods are urgently needed to be determined. In this study, we compared the heterogeneity of the human MSCs derived from bone marrow (BMSCs), umbilical cord (UCMSCs), and adipose tissue (ADSCs) in accelerating wound healing and promoting angiogenesis and explored the underlying mechanism. Briefly, a diabetic rat model with a full-thickness cutaneous wound on the dorsal foot was developed.

Genomic and Phylogenetic Analysis of a Multidrug-Resistant -carrying in China.

Objective: is an emerging hospital-acquired bacterial pathogen. However, there is a dearth of knowledge on the antimicrobial resistance and transmission of . Here, we characterized the microbiological and genomic features of a carbapenem-resistant strain harboring the gene in China.

Identification of Metastasis-Associated Genes in Cutaneous Squamous Cell Carcinoma Based on Bioinformatics Analysis and Experimental Validation.

Introduction: Cutaneous squamous cell carcinoma (cSCC) is a global malignant tumor with a high degree of malignancy. Once metastasis occurs, it will lead to poor prognosis and even death. This study attempts to find out the central genes closely related to cSCC metastasis, so as to clarify the molecular regulatory mechanism of cSCC metastasis and open up new ideas for clinical treatment.

First Identification of a Multidrug-Resistant Co-Carrying Five β-Lactam Resistance Genes Recovered from a Urinary Tract Infection in China.

The emergence of multidrug-resistant spp. in the clinical settings has heightened public awareness. Here, we described the genomic characteristics of a isolate co-carrying five β-lactam resistance genes recovered from a urinary tract infection in China.

Blocking circ-CNST suppresses malignant behaviors of osteosarcoma cells and inhibits glycolysis through circ-CNST-miR-578-LDHA/PDK1 ceRNA networks.

Background: CircRNA CNST (circ-CNST) is a newly identified biomarker for prognosis of osteosarcoma (OS). However, its role in OS progression remains to be well documented.

Methods: Expression of circ-CNST, microRNA (miR)-578, lactate dehydrogenase A (LDHA), and pyruvate dehydrogenase kinase 1 (PDK1) was detected by quantitative real-time polymerase chain reaction and Western blotting.

Carbon Arc Lamp Therapy Enhances the Repair Potential of Chronic Soft Tissue Injury in Rats Compared with the Ability of Photobiomodulation Therapy.

The effects of photobiomodulation therapy (PBMT) and carbon arc lamp therapy (CALT) on the repair of chronic soft tissue injury were compared. PBMT improves soft tissue repair of chronic injury. However, there has been no research on the effect of CALT.

[Clinical research progress of mesenchymal stem cells in treatment of chronic wounds].

Objective: To review the clinical research progress of mesenchymal stem cells (MSCs) in the treatment of chronic wounds.

Methods: The literature related to the chronic wound repair with MSCs at home and abroad in recent years was extensively reviewed, and the possible mechanism of MSCs in the treatment of chronic wounds, as well as its application and existing problems were summarized.

Results: MSCs can participate in all aspects of chronic wound healing to promote wound healing, and has shown broad application prospects in clinical trials.

Efficacy of topical and systemic transplantation of mesenchymal stem cells in a rat model of diabetic ischemic wounds.

Background: Mesenchymal stem cells (MSCs) exert positive effects in chronic wounds. However, critical parameters, such as the most effective administration routes, remain unclear. Accordingly, the purpose of this study was to compare the effects of topical and systemic transplantation MSCs on diabetic ischemic wound healing and explored the underlying mechanisms.

De Winter syndrome may be an early electrocardiogram pattern of acute myocardial infarction, two cases report.

Background: De Winter syndrome is an electrocardiogram (ECG) pattern related to acute occlusion of the anterior descending artery. The incidence rate of De Winter syndrome is rare, but still requires much attention from clinicians.

Methods: Two patients who finnaly diagnosed with De Winter syndrome were included in our study.

Allogeneic Platelet-Rich Plasma Therapy as an Effective and Safe Adjuvant Method for Chronic Wounds.

Background: Platelet-rich plasma (PRP) improves the healing of refractory wounds, and its application is receiving more attention in the field of wound repair. However, when a patient's condition is very poor, it may be difficult to provide whole blood to harvest autologous PRP.

Methods: We evaluated the efficacy and safety of allogeneic PRP in the field of chronic refractory wound repair.

Effects of Carbon Arc Lamp Irradiation on Wound Healing in a Rat Cutaneous Full-Thickness Wound Model.

The objective of the present study was to investigate the application of a carbon arc lamp on wound healing in a rat cutaneous full-thickness wound model. In clinical practice, wound healing has been promoted by irradiation with a carbon arc lamp. However, the corresponding mechanism has not been clearly defined.

Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use.

Adipose‑derived stem cells (ADSCs) are mesenchymal stem cells that are often used in regenerative medicine. Maintaining ADSC viability is important, as this optimizes the curative effects of cell therapy. However, the optimal conditions for cell viability preservation remain unknown.

A novel role of angiotensin II in epidermal cell lineage determination: Angiotensin II promotes the differentiation of mesenchymal stem cells into keratinocytes through the p38 MAPK, JNK and JAK2 signalling pathways.

Background: Recent evidence suggests that angiotensin II (Ang II) plays a role in cutaneous wound healing. Mesenchymal stem cells (MSCs) are known as a rich source of cells that re-establish healed skin. However, the potential impact of Ang II on MSC differentiation into keratinocytes is still unknown.

Effects of Carbon Arc Lamp Irradiation on Wound Healing in a Rat Cutaneous Full-Thickness Wound Model.

Objective: The objective of the present study was to investigate the application of a carbon arc lamp on wound healing in a rat cutaneous full-thickness wound model.

Background Data: In clinical practice, wound healing has been promoted by irradiation with a carbon arc lamp. However, the corresponding mechanism has not been clearly defined.

The mechanical anisotropy on a longitudinal section of human enamel studied by nanoindentation.

Nanoindentation has been widely used for probing the mechanical properties of tooth, especially for characterizing its complex hierarchical structures. Previous studies have confirmed the anisotropic mechanical behaviors caused by the alternated orientations of enamel rods and the alignment of fibril-like hydroxyapatite crystals, but the longitudinal section of enamel, which was composed of parallel-arranged rods, was regarded as a homogeneous continuum as always. In this study, nanoindentation combined with SEM was carried out with the indenter rotating on the longitudinal section of enamel to evaluate the relativity between the nano-mechanical properties and the orientation of indentation impressions.

The enamel softening and loss during early erosion studied by AFM, SEM and nanoindentation.

Enamel dissolution occurs when it contacts with acids produced by plaque bacteria, foods or drinks. There have been numerous and varied studies quantifying and characterizing the rate, extent and chemical aspects of enamel erosion; however, there is still hot debate about the amounts of enamel softening and loss. The objective of this study was to measure the enamel erosion process with high accuracy.

[The comparative study of the effect of silica and calcium carbonate based dentifrice on dentine abrasives in vitro].

Purpose: To evaluate the effect of Silica (SiO(2)) and Calcium Carbonate (CaCO(3)) based dentifrice on their dentine abrasives.

Methods: Microscope, environmental electron scanning electron microscope (SEM) and radioactive Dentine Abrasion (RDA) assay were used to quantitatively measure the wear of dentine.

Results: The mechanical brushing assay showed severe dentine abrasion with CaCO(3) based dentifrices.