Label-free nucleic acid fluorescent probes are becoming more popular because they are cost-effective and easy to use, but they often struggle with low specificity due to interactions with other biomolecules.
The researchers developed a new detection platform using FeO@PPy and label-free probes like TTAPE or Malachite Green, allowing for highly selective detection of various targets such as metal ions, acetamiprid, and thrombin.
The method demonstrated strong sensitivity and selectivity in real samples, achieving low detection limits for acetamiprid in vegetables, thrombin in serum, and lead in water, making it a promising tool for clinical and environmental testing.