Macrogenomic analysis of tolerance and degradation mechanisms of polycyclic aromatic hydrocarbon in carbon and nitrogen metabolic pathways and associated bacterial communities during endogenous partial denitrification.

The effective production of NO-N through endogenous partial denitrification (EPD) provides a promising perspective for the broader adoption and application of anaerobic ammonia oxidation. However, the accumulation of polycyclic aromatic hydrocarbons (PAHs) in the environment may worsen the operational challenges of the EPD system. This study evaluated the resilience of the EPD system to the toxic impacts of phenanthrene (PHE) and anthracene (ANT) through macrogenomic analysis.

Responses of endogenous partial denitrification process to acetate and propionate as carbon sources: Nitrite accumulation performance, microbial community dynamic changes, and metagenomic insights.

Endogenous partial denitrification (EPD) offered a promising pathway for supplying nitrite to anammox, and it also enabled energy-efficient and cost-effective nitrogen removal. However, information about the impact of different carbon sources on the EPD system was limited, and the metabolic mechanisms remained unclear. This study operated the EPD system for 180 days with various acetate and propionate ratios over eight phases.

Comparation on the responses and resilience of single-Anammox system and synergistic partial-denitrification/anammox system to long-term nutrient starvation: Performance and metagenomic insights.

Starvation disturbance was a common problem in biological sewage treatment processes. However, understanding about the responses and resilience of different active anammox biomass in autotrophic and heterotrophic systems to long-term nutrient starvation remains limited. This study compared responses and potential recovery mechanisms of autotrophic single-Anammox and heterotrophic synergistic partial-denitrification/anammox (PD/anammox) systems to prolonged starvation (31-40 days).

Robustness of the synergistic partial-denitrification, anammox, and fermentation process for treating domestic and nitrate wastewaters under fluctuating C/N ratios.

The synergistic partial-denitrification, anammox, and fermentation (SPDAF) process presents a promising solution to treat domestic and nitrate wastewaters. However, its capability to handle fluctuating C/N ratios (the ratios of COD to total inorganic nitrogen) in practical applications remains uncertain. In this study, the SPDAF process was operated for 236 days with C/N ratios of 0.

Insight into the potentiality of nano zero-valent iron on enhancing the nitrite accumulation and phosphorus removal performance of endogenous partial denitrification systems.

Endogenous partial denitrification (EPD) has drawn a lot of interest due to its abundant nitrite (NO-N) accumulation capacity. However, the poor phosphate (PO-P) removal rate of EPD restricts its promotion and application. In this study, the potentiality of various nano zero-valent iron (nZVI) concentrations (0, 20, 40, and 80 mg/L) on NO-N accumulation and PO-P removal in EPD systems had been investigated.

Simultaneous phosphorus and nitrogen removal with different C/N ratios in a low oxygen aeration system: Microorganisms and mechanisms.

In this study, a combined system with simultaneous nitrification, denitrification, and phosphorus removal was operated in continuous low oxygen aeration mode, and the effect of lower oxygen aeration (dissolved oxygen [DO] 0.5-1.5 mg/L) on its performance was examined.

Rapid and stable achievement of mainstream nitritation at low temperature using the competitive inhibition caused by the organics.

The unstability of mainstream nitritation at low temperature has always been a limiting factor for the nitrogen removal from domestic wastewater by anammox. In this study, the competitive inhibition caused by the organics has been demonstrated as an effective strategy to rapidly and stably achieve mainstream nitritation. This strategy was based on our novel discovery that nitrite-oxidizing bacteria (NOB) was more susceptible to the organics inhibition than ammonia-oxidizing bacteria (AOB).

Synergistic partial denitrification, anammox and in-situ fermentation (SPDAF) process for treating domestic and nitrate wastewater: Response of nitrogen removal performance to decreasing temperature.

A synergistic partial denitrification, anaerobic ammonium oxidation (Anammox), and in-situ fermentation (SPDAF) system was established to solve problems of wastewater treatment plants (WWTPs) in combined treatment of domestic sewage, and nitrate wastewater discharged from industrial areas. The SPDAF system was started up at decreasing temperatures (26.8-18.

Advanced nitrogen and phosphorus removal from municipal wastewater via simultaneous enhanced biological phosphorus removal and semi-nitritation (EBPR-SN) combined with anammox.

In this study, a novel laboratory-scale synchronous enhanced biological phosphorus removal and semi-nitritation (termed as EBPR-SN) combined with anammox process was put forward for achieving nutrient elimination from municipal wastewater at 27 ℃. This process consisted of two 10 L sequencing batch reactors (SBRs), i.e.

A novel partial nitrification-synchronous anammox and endogenous partial denitrification (PN-SAEPD) process for advanced nitrogen removal from municipal wastewater at ambient temperatures.

Mainstream anammox still faces the challenges of non-ideal NO/NH ratios and excess nitrate resulted from the instability of partial nitrification (PN) in municipal wastewater. To address these problems, in this study, we developed a novel two-sludge process that combined PN with synchronous anammox and endogenous partial denitrification (SAEPD); the process was tested with pre-treated domestic sewage at ambient temperatures for 205 d. High nitrogen removal efficiency of 91.

Synergistic Partial-Denitrification, Anammox, and in-situ Fermentation (SPDAF) Process for Advanced Nitrogen Removal from Domestic and Nitrate-Containing Wastewater.

This study presents a new method for energy-efficient wastewater treatment that synergizes the partial-denitrification, anammox, and in-situ fermentation (SPDAF) processes in an up-flow reactor. Nitrate-containing wastewater and actual domestic sewage were fed into this SPDAF system, which was operated for 180 days without the addition of external carbon sources and aeration. The total inorganic nitrogen (TIN) removal efficiency reached 93.

[Effect of Cu on Denitrification Using NO as an Electron Acceptor].

The short-cut biological nitrogen removal process has been widely used in industrial wastewater treatment, and denitrification is a crucial step for removing nitrogen on which the effect of Cu, a common heavy metal ion in wastewater, has not been studied. In this study, sludge with good short-range biological nitrogen removal characteristics in an A/O reactor was selected to investigate the short-term and long-term effects of Cu on denitrification using NO as an electron acceptor. The results showed that Cu had a significant inhibitory effect on denitrification process using NO as an electron acceptor, and the semi-inhibitory concentration EC of sludge activity was 4.

A novel SNPR process for advanced nitrogen and phosphorus removal from mainstream wastewater based on anammox, endogenous partial-denitrification and denitrifying dephosphatation.

For achieving energy-efficient wastewater treatment, a novel simultaneous nitrogen and phosphorus removal (SNPR) process, which integrated anammox, endogenous partial-denitrification and denitrifying dephosphatation in a sequencing batch reactor with granular sludge was developed to treat mainstream wastewater. After 200 days of operation, a simultaneous high-level nitrogen and phosphorus removal of 93.9% and 94.

Partial denitrification providing nitrite: Opportunities of extending application for anammox.

Anaerobic ammonium oxidation (anammox) has been extensively investigated for cost-efficient nitrogen removal from wastewater. However, the major issues of nitrate (NO-N) residue and instability in the current combination of nitritation and anammox process necessitates being addressed efficiently. The recently proposed partial-denitrification (PD), terminating NO-N reduction to nitrite (NO-N), has been regarded as a promising alternative of NO-N supplying for anammox bacteria.

Stable long-term operation and high nitrite accumulation of an endogenous partial-denitrification (EPD) granular sludge system under mainstream conditions at low temperature.

In this study, the robustness of an endogenous partial-denitrification (EPD) system was demonstrated during a 105 days operation under mainstream conditions at low temperature. The results showed a high nitrate-to-nitrite transformation ratio (NTR) of 88.7%, a suitable NO-N/NH-N ratio of 1.

Achieving advanced nitrogen removal from low C/N wastewater by combining endogenous partial denitrification with anammox in mainstream treatment.

Successful application of mainstream anammox would be favorable for energy- and resource-efficient sewage treatment. This study presents a new strategy to achieve mainstream anammox, which combined with endogenous partial denitrification (EPD) for treating sewage wastewater. In this EPD-Anammox system, nitrite was stably produced by EPD with a nitrate-to-nitrite transformation ratio of 80%.

Effects of salinity build-up on the performance and microbial community of partial-denitrification granular sludge with high nitrite accumulation.

High inorganic salts inevitably impose a toxic impact on biological treatment processes. In this study, the effect of salinity on the performance and microbial community structures of partial-denitrification (PD) was firstly investigated. Results showed the denitrifying activities of non-domesticated PD sludge were completely inhibited under a temporary high salinity (≥1.

Achievement of high nitrite accumulation via endogenous partial denitrification (EPD).

This study proposed a novel strategy for achievement of partial denitrification driven by endogenous carbon sources in an anaerobic/anoxic/aerobic activated sludge system. Results showed that in the steady-stage, the nitrate-to-nitrite transformation ratio (NTR) was kept at around 87% without nitrate in the effluent. During the anaerobic period, exogenous carbon sources was completely taken up, accompanied by the consumption of glycogen and production of polyhydroxyalkanoates (PHAs).