Root exudate-mediated assemblage of rhizo-microbiome enhances Fusarium wilt suppression in chrysanthemum.

Intercropping is emerging as a sustainable strategy to manage soil-borne diseases, yet the underlying mechanisms remain largely elusive. Here, we investigated how intercropping chrysanthemum (Chrysanthemum morifolium) with ginger (Zingiber officinale) suppressed Fusarium wilt and influenced the associated rhizo-microbiome. Chrysanthemum plants in intercropping systems exhibited a marked reduction in wilt severity and greater biomass compared to those grown in monoculture.

A Novel-Potential Wave-Bump Yarn of Plain Weave Fabric for Fog Harvesting.

With the variety of fibers and fabrics, the studies of the surface structure of the textile yarns, the weave fabric, and their surface wettability are still potential factors to improve and optimize the fog harvesting efficiency. In this work, inspired by the fog harvesting behavior of the desert beetle dorsal surface, a wavy-bumpy structure of post-weave yarn (obtained from woven fabric) was reported to improve large droplet growth (converge) efficiency. In which, this study used tetrabutyl titanate (Ti(OCH)) to waterproof, increase hydrophobicity, and stabilize the surface of yarns and fabric (inspired by the feather structure and lotus leaf surface).

Correction: Zeng et al. Research on Aviation Safety Prediction Based on Variable Selection and LSTM. 2023, , 41.

In the original publication [...

Citrus yellow vein clearing virus infection triggers phloem remobilization of iron- and zinc-nicotianamine in citrus.

Citrus yellow vein clearing virus (CYVCV) is a worldwide and highly destructive disease of citrus, but the mechanisms involved in CYVCV-inhibited plant growth are not well understood. This study examined nutrient levels and their cellular distribution in different organs of healthy and CYVCV-affected citrus (Citrus reticulata 'Kanpei') plants. We found that CYVCV-infected plants exhibit characteristic symptoms, including a significant reduction in iron (Fe) and other elemental nutrients in the shoots.

Three-dimensional reconstruction of insect chemosensory sensillum.

The olfactory system is involved in food and mate recognition in insects. However, 3D structures of chemosensory sensilla in insects are unexplored yet. Here, the internal structures of an olfactory sensillum on the antenna of the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae), one of the most important rice pests, are examined and imaged using focused ion beam scanning electron microscopy.

Starvation-induced phosphorylation activates gasdermin A to initiate pyroptosis.

Pyroptosis, a pro-inflammatory form of programmed cell death, is crucial for host defense against pathogens and danger signals. Proteolytic cleavage of gasdermin proteins B-E (GSDMB-GSDME) is well established as a trigger for pyroptosis, but the intracellular activation mechanism of GSDMA remains elusive. Here, we demonstrate that severe starvation induces pyroptosis through phosphorylation-induced activation of GSDMA.

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Ramie is a widely used plant fiber for making textiles and reinforcement in biodegradable composites. Pretreating cellulosic fibers with alkali before producing composites is increasingly used to enhance adhesion with polymeric resin. In this work, response surface methodology (RSM) based on the Box-Behnken technique was utilized to investigate the impact of independent variables on ramie fabric characteristics and determine the optimal treatment condition.

LncRNA WFDC21P interacts with SEC63 to promote gastric cancer malignant behaviors by regulating calcium homeostasis signaling pathway.

Background: Gastric cancer is currently estimated to be the fifth leading common cancer in the world, and responsible for about one million new cases and an estimated 769,000 cancer-related deaths each year. WFDC21P is long non-coding RNA and has been reported to play critical roles in serval types of cancer. Our research aims to investigate the biological effects and molecular mechanism of WFDC21P in gastric cancer.

A meta-analysis of the clinicopathological significance of the lncRNA MALAT1 in human gastric cancer.

Background: Dysregulation of the long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been linked to some oncogenic pathways that induce cancer initiation and progression. This meta-analysis was conducted to specifically summarize the most recent research on MALAT1 function in human gastric cancer (GC).

Methods: The eligible studies were first identified by searching HowNet, Web of Science, PubMed, The Cochrane Library, Embase, and databases for studies published as of April 1, 2023.

Aero-Engine Remaining Useful Life Prediction Based on Bi-Discrepancy Network.

Most unsupervised domain adaptation (UDA) methods align feature distributions across different domains through adversarial learning. However, many of them require introducing an auxiliary domain alignment model, which incurs additional computational costs. In addition, they generally focus on the global distribution alignment and ignore the fine-grained domain discrepancy, so target samples with significant domain shifts cannot be detected or processed for specific tasks.

Bioinspired Asymmetric Polypyrrole Membranes with Enhanced Photothermal Conversion for Highly Efficient Solar Evaporation.

Solar-driven interfacial evaporation (SDIE) has attracted great attention by offering a zero-carbon-emission solution for clean water production. The manipulation of the surface structure of the evaporator markedly promotes the enhancement of light capture and the improvement of evaporation performance. Herein, inspired by seedless lotus pod, a flexible pristine polypyrrole (PPy) membrane with macro/micro-bubble and nanotube asymmetric structure is fabricated through template-assisted interfacial polymerization.

Modeling the 3-dimensional structure of the silkworm's spinning apparatus in silk production.

The silk-spinning process of the silkworms transforms the liquid silk solution to a solid state under mild conditions, making it an attractive model for bioinspiration However, the precise mechanism behind silk expulsion remains largely unknown. Here we selected the silkworms as representative models to investigate the silk-spinning mechanism. We used serial block-face scanning electron microscopy (SBF-SEM) to reconstruct the three-dimensional structures of the spinnerets in silkworms at various stages and with different gene backgrounds.

Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM.

The oocyte cumulus complex is mainly composed of an oocyte, the perivitelline space, zona pellucida and numerous granulosa cells. The cumulus granulosa cells (cGCs) provide a particularly important microenvironment for oocyte development, regulating its growth, maturation and meiosis. In this study, we studied the internal structures and cell-to-cell connections of mouse cGCs using focused ion beam scanning electron microscopy (FIB-SEM).

Volume electron microscopy reconstruction uncovers a physical barrier that limits virus to phloem.

Most plant reoviruses are phloem-limited, but the mechanism has remained unknown for more than half a century. Southern rice black-streaked dwarf virus (Fijivirus, Reoviridae) causes phloem-derived tumors, where its virions, genomes, and proteins accumulate, and it was used as a model to explore how its host plant limits the virus within its phloem. High-throughput volume electron microscopy revealed that only sieve plate pores and flexible gateways rather than plasmodesmata had a sufficiently large size exclusion limit (SEL) to accommodate virions and potentially serve as pathways of virion movement.

VAPB-mediated ER-targeting stabilizes IRS-1 signalosomes to regulate insulin/IGF signaling.

The scaffold protein IRS-1 is an essential node in insulin/IGF signaling. It has long been recognized that the stability of IRS-1 is dependent on its endomembrane targeting. However, how IRS-1 targets the intracellular membrane, and what type of intracellular membrane is actually targeted, remains poorly understood.

FIB-SEM combined with proteomics and modification omics clarified mechanisms of lipids synthesis in organelles of Chlorella pyrenoidosa cells with high CO concentration.

In order to explain reasons why flue-gas CO (normally containing high CO) enhanced carbon fixation and lipids synthesis with increased photochemical electron production in microalgae cells. Focused ion beam scanning electron microscopy (FIB-SEM) was combined with proteomics and phosphorylation modification mics to clarify mechanisms of lipids synthesis at protein and organelle levels in Chlorella pyrenoidosa cells cultivated with high CO concentration (15 % v/v). The volumes of chloroplast and endoplasmic reticulum in subcellular organelles increased by 47 % and 306 %, respectively, compared with the control, which improved conversion efficiency of starch grains to lipids (lipid content increased by 57 %).

Quantification of plasmodesmata frequency under three-dimensional view using focused ion beam-scanning electron microscopy and image analysis.

The quantitative study of plasmodesmata (PD) frequency is routine in plant science for providing information on the potential of intercellular transportation. Here, we report quantification of plasmodesmatal frequency in virus-infected tobacco vascular tissues using serial sectioning and image analysis. The image datasets were collected by focused ion beam-scanning electron microscopy (FIB-SEM), and the measurements of plasmodesmatal frequency were performed after image analysis with commercial computational programs.

Research on Aviation Safety Prediction Based on Variable Selection and LSTM.

Accurate prediction of aviation safety levels is significant for the efficient early warning and prevention of incidents. However, the causal mechanism and temporal character of aviation accidents are complex and not fully understood, which increases the operation cost of accurate aviation safety prediction. This paper adopts an innovative statistical method involving a least absolute shrinkage and selection operator (LASSO) and long short-term memory (LSTM).

Three-dimensional analysis of membrane structures associated with tomato spotted wilt virus infection.

To study viral infection, the direct structural visualization of the viral life cycle consisting of virus attachment, entry, replication, assembly and transport is essential. Although conventional electron microscopy (EM) has been extremely helpful in the investigation of virus-host cell interactions, three-dimensional (3D) EM not only provides important information at the nanometer resolution, but can also create 3D maps of large volumes, even entire virus-infected cells. Here, we determined the ultrastructural details of tomato spotted wilt virus (TSWV)-infected plant cells using focused ion beam scanning EM (FIB-SEM).

FIB-SEM analysis on three-dimensional structures of growing organelles in wild Chlorella pyrenoidosa cells.

To clarify dynamic changes of organelle microstructures in Chlorella pyrenoidosa cells during photosynthetic growth with CO fixation, three-dimensional (3D) organelle microstructures in three growth periods of meristem, elongation, and maturity were quantitatively determined and comprehensively reconstructed with focused ion beam scanning electron microscopy (FIB-SEM). The single round-pancake mitochondria in each cell split into a dumbbell and then into a circular ring, while the barycenter distance of mitochondria to chloroplast and nucleus was reduced to 45.5% and 88.

BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency.

Primary ovarian insufficiency (POI) is a clinical syndrome of ovarian dysfunction characterized by premature exhaustion of primordial follicles. POI causes infertility, severe daily life disturbances and long-term health risks. However, the underlying mechanism remains largely unknown.

Flooding-induced rhizosphere Clostridium assemblage prevents root-to-shoot cadmium translocation in rice by promoting the formation of root apoplastic barriers.

Water managements are the most effective agricultural practices for restraining cadmium (Cd) uptake and translocation in rice, which closely correlated with rhizosphere assembly of beneficial microbiome. However, the role of the assemblage of specific microbiota in controlling root-to-shoot Cd translocation in rice remains scarcely clear. The aim of this study was to ascertain how water managements shaped rhizosphere microbiome and mediated root-to-shoot Cd translocation.