Endothelial endogenous CSE/HS inhibits endothelial pyroptosis by activating sirtuin1 to attenuate LPS-induced acute lung injury.

Endothelial pyroptosis, a pro-inflammatory programmed cell death, promotes endothelial inflammation and is a pivotal process in the initial stage of acute lung injury (ALI). Hydrogen sulfide (HS), a gasotransmitter primarily dependent on cystathionine γ-lyase (CSE) in the cardiovascular and respiratory systems, plays a protective role during ALI. Nonetheless, the modulatory role and precise molecular mechanism of endothelial endogenous CSE/HS in the pathogenesis of ALI remain elusive.

Determination and Pharmacokinetics of Acetylcorynoline in Mouse Plasma by UPLC-MS/MS.

Acetylcorynoline is an alkaloid isolated from the tubers of Cham. et Schltdl. It has anti-inflammatory properties with the potential to treat Parkinson's disease.

Wide-field imaging and recognition through cascaded complex scattering media.

Considering the obvious application value in the field of minimally invasive and non-destructive clinical healthcare, we explore the challenge of wide-field imaging and recognition through cascaded complex scattering media, a topic that has been less researched, by realizing wide-field imaging and pathological screening through multimode fibers (MMF) and turbid media. To address the challenge of extracting features from chaotic and globally correlated speckles formed by transmitting images through cascaded complex scattering media, we establish a deep learning approach based on SMixerNet. By efficiently using the parameter-free matrix transposition, SMixerNet achieves a broad receptive field with less inductive bias through concise multi-layer perceptron (MLP).

915 nm pumping kilowatt fiber oscillator with high optical-to-optical efficiency.

We demonstrate an all-fiber oscillator with high optical-to-optical efficiency using laser diodes working at 915nm as the pump sources to reduce the demand for thermal management. When the output power of the bidirectional pumped oscillator is 1.16kW, the optical-to-optical efficiency is as high as 75.

Research on multi-heat source arrangement optimization based on equivalent heat source method and reconstructed variational autoencoder.

The variational autoencoder (VAE) architecture has significant advantages in predictive image generation. This study proposes a novel RFCNN-βVAE model, which combines residual-connected fully connected neural networks with VAE to handle multi-heat source arrangements. By integrating analytical solutions, polynomial fitting, and temperature field superposition, we accurately simulated the temperature rise distribution of a single heat source.

Molecular prevalence, subtype distribution, and zoonotic potential of sp. in wild rodents and shrews inhabiting Zhejiang province of China.

Introduction: Globally, rodents and shrew populations constitute crucial elements of diverse environments and animal communities. It is imperative to study their population dynamics to mitigate any potential negative impact on humans, as they can be involved in the transmission of critical zoonotic agents, such as . Therefore, this study aimed to identify the prevalence and genetic composition of in wild rodents and shrews residing in the Zhejiang provinces of China.

Quantification of Rhodojaponin II and Rhodojaponin III in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry.

An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to determine the concentrations of Rhodojaponin II and Rhodojaponin III in rat plasma, and their pharmacokinetic profiles were investigated. A UPLC HSS T3 (2.1 mm × 50 mm, 1.

CFZA camera: a high-resolution lensless imaging technique based on compound Fresnel zone aperture.

In lensless imaging using a Fresnel zone aperture (FZA), it is generally believed that the resolution is limited by the outermost ring breadth of the FZA. The limitation has the potential to be broken according to the multi-order property of binary FZAs. In this Letter, we propose to use a high-order component of the FZA as the point spread function (PSF) to develop a high-order transfer function backpropagation (HBP) algorithm to enhance the resolution.

Super-resolution lensless on-chip microscopy based on array illumination and sub-pixel shift search.

The resolution of a lensless on-chip microscopy system is constrained by the pixel size of image sensors. This Letter introduces a super-resolution on-chip microscopy system based on a compact array light source illumination and sub-pixel shift search. The system utilizes a closely spaced array light source composed by four RGB LED modules, sequentially illuminating the sample.

Wide-field color imaging through multimode fiber with single wavelength illumination: plug-and-play approach.

Multimode fiber (MMF) is extensively studied for its ability to transmit light modes in parallel, potentially minimizing optical fiber size in imaging. However, current research predominantly focuses on grayscale imaging, with limited attention to color studies. Existing colorization methods often involve costly white light lasers or multiple light sources, increasing optical system expenses and space.

Investigating the Effects of Full-Spectrum LED Lighting on Strawberry Traits Using Correlation Analysis and Time-Series Prediction.

In crop cultivation, particularly in controlled environmental agriculture, light quality is one of the most critical factors affecting crop growth and harvest. Many scholars have studied the effects of light quality on strawberry traits, but they have used relatively simple light components and considered only a small number of light qualities and traits in each experiment, and the results were not complete or objective. In order to comprehensively investigate the effects of different light qualities from 350 nm to 1000 nm on strawberry traits to better predict the future growth trend of strawberries under different light qualities, we proposed a new approach.

Hydrogen sulfide ameliorates lipopolysaccharide-induced anxiety-like behavior by inhibiting checkpoint kinase 1 activation in the hippocampus of mice.

Hydrogen sulfide (HS), an endogenous gasotransmitter, exhibits the anxiolytic roles through its anti-inflammatory effects, although its underlying mechanisms remain largely elusive. Emerging evidence has documented that cell cycle checkpoint kinase 1 (Chk1)-regulated DNA damage plays an important role in the neurodegenerative diseases; however, there are few relevant reports on the research of Chk1 in neuropsychiatric diseases. Here, we aimed to investigate the regulatory role of HS on Chk1 in lipopolysaccharide (LPS)-induced anxiety-like behavior focusing on inflammasome activation in the hippocampus.

An Approach to Improve Energy Efficiency during Antimicrobial Blue Light Inactivation: Application of Pulse-Width Modulation Dimming to Balance Irradiance and Irradiation Time.

Antimicrobial blue light (aBL) is an effective non-destructive inactivation technique and has received increasing attention. Despite its significance, the existing research has not thoroughly delved into the impacts of irradiance and irradiation time on enhancing energy efficiency during aBL inactivation and the explanation of the enhancement effect of pulse exposure. In this paper, a series of inactivation experiments with different duty cycles, pulse frequencies, and irradiation times were conducted, and the relative concentrations of reactive oxygen species (ROS) were measured under corresponding conditions.

Amp-vortex edge-camera: a lensless multi-modality imaging system with edge enhancement.

We demonstrate a lensless imaging system with edge-enhanced imaging constructed with a Fresnel zone aperture (FZA) mask placed 3 mm away from a CMOS sensor. We propose vortex back-propagation (vortex-BP) and amplitude vortex-BP algorithms for the FZA-based lensless imaging system to remove the noise and achieve the fast reconstruction of high contrast edge enhancement. Directionally controlled anisotropic edge enhancement can be achieved with our proposed superimposed vortex-BP algorithm.

A Review of System-in-Package Technologies: Application and Reliability of Advanced Packaging.

The system-in-package (SiP) has gained much interest in the current rapid development of integrated circuits (ICs) due to its advantages of integration, shrinking, and high density. This review examined the SiP as its focus, provides a list of the most-recent SiP innovations based on market needs, and discusses how the SiP is used in various fields. Reliability issues must be resolved if the SiP is to operate normally.

Extended angular-spectrum modeling (EASM) of light energy transport in scattering media.

The exact modeling of light transport in scattering media is critical in biological imaging, free-space communication, and phosphor-converted lighting. Angular spectrum is proved to be a fast and effective approach to reconstructing the wavefront dynamics during the propagation in scattering media, however, finding it difficult in acquiring the wavefront and energy change simultaneously. Besides, conventional methods for energy tracing, such as the Monte Carlo method, are inefficient in speed and hard to simulate the wavefront change.

High extraction efficiency phosphor design applied in laser lighting.

Laser lighting has great potential to be the next generation of general lighting due to its high brightness and directionality. However, the light extraction efficiency and luminous efficiency from the light exit surface are greatly limited since phosphor structure. Here, we design and optimize a phosphor structure by Monte Carlo method (MCM) with optimization algorithm.

Nyquist Sampling Conditions of Some Diffraction Algorithms with Adjustable Magnification.

Diffraction algorithms with adjustable magnification are dominant in holographic projection and imaging. However, the algorithms are limited by the Nyquist sampling conditions, and simulation results with inappropriate parameters sometimes appear with aliasing. At present, many diffraction algorithms have been proposed and improved, but there is a need for an overall analysis of their sampling conditions.

VGLL4-TEAD1 promotes vascular smooth muscle cell differentiation from human pluripotent stem cells via TET2.

The Hippo signaling pathway plays a critical role in cardiovascular development and stem cell differentiation. Using microarray profiling, we found that the Hippo pathway components vestigial-like family member 4 (VGLL4) and TEA domain transcription factor 1 (TEAD1) were upregulated during vascular smooth muscle cell (VSMC) differentiation from H1 ESCs (H1 embryonic stem cells). To further explore the role and molecular mechanisms of VGLL4 in regulating VSMC differentiation, we generated a VGLL4-knockdown H1 ESC line (heterozygous knockout) using the CRISPR/Cas9 system and found that VGLL4 knockdown inhibited VSMC specification.

A singlet state oxygen generation model based on the Monte Carlo method of visible antibacterial blue light inactivation.

Visible antibacterial blue light (VABL) has received much attention recently as a nondestructive inactivation approach. However, due to the sparse distribution of bacteria, the light energy evaluation method used in existing studies is inaccurate. Thus, the sensitivity of microorganisms to VABL in different experiments cannot be compared.

Correction: Wu et al. Full-Color See-Through Three-Dimensional Display Method Based on Volume Holography. 2021, , 2698.

The authors make the following corrections to the published paper [...

YAP inhibition promotes endothelial cell differentiation from pluripotent stem cell through EC master transcription factor FLI1.

Endothelial cells (ECs) derived from pluripotent stem cells (PSCs) provide great resource for vascular disease modeling and cell-based regeneration therapy. However, the molecular mechanisms of EC differentiation are not completely understood. In this study, we checked transcriptional profile by microarray and found Hippo pathway is changed and the activity of YAP decreased during mesoderm-mediated EC differentiation from human embryonic stem cells (hESCs).

Modeling of UV diffused-LOS communication channel incorporating obstacle and its applicability analysis.

Existing studies of the ultraviolet (UV) channel mainly focus on non-line-of-sight (NLOS) scenarios, while line-of-sight (LOS) scenarios are generally neglected since obstacles sometimes block them. To fill this gap, a UV diffused-LOS channel model in the presence of an obstacle is proposed for the first time, to the best of our knowledge. For easy interpretation, the whole derivation is combined with four typical positions of the obstacle.

Critical role of VGLL4 in the regulation of chronic normobaric hypoxia-induced pulmonary hypertension in mice.

Pulmonary hypertension (PH), a rare but deadly cardiopulmonary disorder, is characterized by extensive remodeling of pulmonary arteries resulting from enhancement of pulmonary artery smooth muscle cell proliferation and suppressed apoptosis; however, the underlying pathophysiological mechanisms remain largely unknown. Recently, epigenetics has gained increasing prominence in the development of PH. We aimed to investigate the role of vestigial-like family member 4 (VGLL4) in chronic normobaric hypoxia (CNH)-induced PH and to address whether it is associated with epigenetic regulation.

Generation of human embryonic stem cell lines (WAe001-A-67,WAe001-A-68) with TEAD1 and TEAD4 expression by the PiggyBac transposon system.

Transcription factors TEAD1 and TEAD4 play an important role in development, differentiation, cell growth and proliferation. To further understand the exact role of TEAD1 and TEAD4 in these processes. We generated TEAD1 and TEAD4 doxycycline-inducible expression human embryonic stem cell lines (WAe001-A-67 and WAe001-A-68) by PiggyBac transposon system.