Study of the ability of polysaccharides isolated from Zizania latifolia to promote wound healing in mice via in vitro screening and in vivo evaluation.

Swollen culms of Zizania latifolia possess a peculiar flavor, nutritional value and health-promoting effects. This study investigated the wound healing ability of polysaccharides isolated from Zizania latifolia (ZLP). Through in vitro assays, ZLP demonstrated negligible cytotoxicity and promoted the migration and phagocytosis of Staphylococcus aureus by macrophages where ZLP-2 had a significantly greater effect.

The isolation, characterization and biological activities of the non-glucan polysaccharides from the high-starch-content plant Pueraria mirifica.

The dried root of Pueraria mirifica (P. mirifica) is an edible foodstuff widely used in Asian countries. P.

Enzymatic synthesis of Vaccinium blue using vaccinoside as a bifunctional precursor.

Since Tang dynasty in China, the fresh leaves of Vaccinium bracteatum (VBL) have been applied as natural pigment to produce black rice. However, detailed information on its biosynthetic mechanism still remained unclear. Following rice dyeing capacity assay, vaccinoside, one of iridoid glycosides, was identified as the key active compound.

[Optimization and application of caprylic acid precipitation in the purification of monoclonal antibody].

In response to the market demand for therapeutic antibodies, the upstream cell culture scale and expression titer of antibodies have been significantly improved, while the production efficiency of downstream purification process is relatively fall behind, and the downstream processing capacity has become a bottleneck limiting antibody production throughput. Using monoclonal antibody mab-X as experimental material, we optimized the caprylic acid (CA) precipitation process conditions of cell culture fluid and low pH virus inactivation pool, and studied two applications of using CA treatment to remove aggregates and to inactivate virus. Based on the lab scale study, we carried out a 500 L scale-up study, where CA was added to the low pH virus inactivation pool for precipitation, and the product quality and yield before and after precipitation were detected and compared.

[Effects of simulated rainfall enhancement on sediment CO flux in dry lakebed of Barkol Lake, China].

Understanding the responses of lake sediment carbon process to climate change is an important part of a comprehensive understanding of lake carbon budget. To explore the effects of future rainfall increase on sediment carbon flux, undisturbed sediment samples were collected from the bottom of dry lake Barikun in Hami, Xinjiang for the incubation experiment. Based on the increase rate of precipitation (4 mm·10 a) and the distribution characteristics of rainfall in the plant growing season in Hami, Xinjiang since 1960, five rainfall treatments were set (86 mm, T; 94 mm, T; 102 mm, T; 110 mm, T; 126 mm, T) based on the rainfall in growing season of 2016 (86 mm).

Geniposide attenuates neonatal mouse brain injury after hypoxic-ischemia involving the activation of PI3K/Akt signaling pathway.

Perinatal hypoxic-ischemia (HI) is a leading cause of acute mortality and neurologic complications in newborns. Geniposide, a natural product extracted from the herb Gardenia jasminoides, has been shown to possess neuroprotective effects in neurologic deficits. This study aims to investigate whether Geniposide has therapeutic potential to HI brain injury and the underlying mechanisms.

Thermal-wave resonant cavity signal processing.

The thermal-wave resonant cavity (TWRC) technique has been used for thermal diffusivity measurements by many researchers. This study aims to reduce the uncertainty associated with TWRC signal processing (curve fitting) by means of numerical simulation and experimental verification. Simulations show that the plot of signal amplitude versus cavity length can be fitted to a simplified model reported previously when the initial fitting position is at least twice the thermal-wave diffusion length (2 μ), and that the uncertainty caused by different end positions is negligible in the range of 6-10 μ.

Correlation of liver function with intestinal flora, vitamin deficiency and IL-17A in patients with liver cirrhosis.

The aim of this study was to investigate the correlation of liver function, intestinal flora, vitamin D and interleukin-17A (IL-17A) levels in patients with liver cirrhosis. A total of 52 patients diagnosed with posthepatitic cirrhosis and admitted into Yantai Infectious Disease Hospital (Yantai, China) from January to December in 2012 (liver cirrhosis group), and 52 patients with chronic hepatitis B (hepatitis group), and 40 healthy volunteers receiving physical examination in the hospital (normal control group) were selected into the study. The liver function, hepatitis B virus (HBV) deoxyribonucleic acid (DNA) level, intestinal flora distribution, vitamin D and IL-17A levels of all patients were detected, and the correlation among them was analyzed via Pearson's analysis.

Frequency analysis of temperature-dependent interferometric signal for the measurement of the temperature coefficient of refractive index.

A method of frequency analysis for the measurement of the temperature coefficient of refractive index (dn/dT) using a Fabry-Perot interferometer was developed and tested against ethanol and water. The temperature-dependent interferometric signal described by Airy's formula was analyzed in both the temperature and frequency domains. By fast Fourier transform, a low-pass filter was designed and employed to eliminate the noise superimposed on the signal.

A simplified model for thermal-wave cavity self-consistent measurement of thermal diffusivity.

A simplified theoretical model was developed for the thermal-wave cavity (TWC) technique in this study. This model takes thermal radiation into account and can be employed for absolute measurements of the thermal diffusivity of gas and liquid samples without any knowledge of geometrical and thermal parameters of the components of the TWC. Using this model and cavity-length scans, thermal diffusivities of air and distilled water were accurately and precisely measured as (2.

Immobilization of cellulase on a reversibly soluble-insoluble support: properties and application.

Cellulase was coupled to N-succinyl-chitosan (NSC) showing soluble-insoluble characteristics with pH change. Cellulase immobilized on NSC (NSCC) is in a soluble state during the enzyme reaction, yet can be recovered in its insoluble form by lowering the pH of the reaction solution after the reaction. NSCC was obtained under the optimized immobilization conditions of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) 10 mg, cellulase 15 mg, and pH 5.

Thermal-lens study of photochemical reaction kinetics.

We consider the time dependence of the absorption coefficient due to the photoinduced chemical reaction (PCR) and species diffusion to calculate the temperature rise in the thermal-lens (TL) effect. The TL signal at the detector plane is also calculated. This theoretical approach removes the restriction that the PCR time constant is much greater than the characteristic TL time constant, which was assumed in a previously published model.

The effect of relative humidity on binary gas diffusion.

The dependence of diffusion coefficient of O2-N2 mixture in the presence of water vapor was experimentally determined as a function of relative humidity (RH) with different temperatures using an in-house made Loschmidt diffusion cell. The experimental results showed that O2-N2 diffusion coefficient increased more than 17% when RH increased from 0% to 80% at 79 degrees C. In the experiments, the RH in both top and bottom chambers of the diffusion cell were the same, and the pressure inside the diffusion cell was kept as ambient pressure (1 atm.

Time-resolved thermal mirror technique with top-hat cw laser excitation.

A theoretical model was developed for time-resolved thermal mirror spectroscopy under top-hat cw laser excitation that induced a nanoscale surface displacement of a low absorption sample. An additional phase shift to the electrical field of a TEM(00) probe beam reflected from the surface displacement was derived, and Fresnel diffraction theory was used to calculate the propagation of the probe beam. With the theory, optical and thermal properties of three glasses were measured, and found to be consistent with literature values.

Peripheral arterial occlusive disease: global gene expression analyses suggest a major role for immune and inflammatory responses.

Background: Peripheral arterial disease (PAD), a major manifestation of atherosclerosis, is associated with significant cardiovascular morbidity, limb loss and death. However, mechanisms underlying the genesis and progression of the disease are far from clear. Genome-wide gene expression profiling of clinical samples may represent an effective approach to gain relevant information.

[Research on the orientedly immobilized urease via concanavalin A].

Concanavalin A (ConA) is immobilized on a pre-activated chitosan microspheres, and then oriented immobilization of urease is carried out based on the strong interaction between ConA and glycoprotein. The optimum immobilization conditions are as follows: glutaraldehyde concentration is 3.5%, ConA concentration 1 mg/mL, ConA pH 7.

Top-hat cw-laser-induced time-resolved mode-mismatched thermal lens spectroscopy for quantitative analysis of low-absorption materials.

Thermal lens spectroscopy is a highly sensitive and versatile photothermal technique for material analysis, providing optical and thermal properties. To use less expensive multimode non-Gaussian lasers for quantitative analysis of low-absorption materials, this Letter presents a theoretical model for time-resolved mode-mismatched thermal lens spectroscopy induced by a cw laser with a top-hat profile. The temperature profile in a sample was calculated, and the intensity of the probe beam center at the detector plane was also derived using the Fresnel diffraction theory.

Chemomicin A, a new angucyclinone antibiotic produced by Nocardia mediterranei subsp. kanglensis 1747-64.

A new angucyclinone antibiotic, chemomicin A was isolated from cultured broth of Nocardia mediterranei subsp. kanglensis 1747-64. Its chemical structure was determined to be 1,2,3,4a,5,6,6a,12a,12b-nonahydro-1,2,3,8,12,12b-hexahydroxy-3-methyl-6a,12a-epoxybenz[a]anthracen-4,7(12H)-dione by a detailed spectroscopic analysis.

Deinococcus yunweiensis sp. nov., a gamma- and UV-radiation-resistant bacterium from China.

A Gram-negative, non-spore-forming, non-motile, rod-shaped, red-pigmented strain, designated YIM 007T, was found as a contaminant on an agar plate in the laboratory of Yunnan Institute of Microbiology, China. The optimum growth pH and temperature for the isolate were 7.0-7.

Preliminary report on the biological effects of space flight on the producing strain of a new immunosuppressant, Kanglemycin C.

Kanglemycin C (K-C) is a new immunosuppressant isolated from the culture broth of Nocardia mediterranei var. kanglensis 1747-64. To improve the productivity of K-C and to study the biological effects of space flight on its producing strain, spores from five K-C producing strains (U-10, U-15, U-7, M-13, gamma-33) mutated from the wild strain N.

Probe beam size effect on the measurement of the distance between the probe beam and the sample in photothermal deflection.

A distance-scan method to determine the distance between the probe beam and sample, which is not easily measured exactly, in photothermal deflection (PD) was reported, with which the distance and thermal diffusivity of the deflecting medium can be simultaneously measured. Probe beam size effect (PBSE) on PD phase signal was quantitatively analyzed to clearly show its physical meaning. The measured distance was experimentally verified as correct and reliable, and the measured thermal diffusivities of N2 and CO2 are in good agreement with the literature values.

[Kinetics model of spherical immobilized cellulase].

A kinetics model was developed for predicting and simulating immobilized cellulase performance, which follows Michaelis-Menten kinetics with competitive product inhibition. Taking into account the effects of competitive product inhibition, inner diffusional limitation, substrate concentration and carrier size, the substrate distribution and the product distribution in carriers were investigated, and the effectiveness factors were also calculated over a wide range of parameters. The effects of competitive product inhibition are shown to increase the substrate concentration in the carrier, and, additionally, to increase the effectiveness factors slightly.

Effective normalization method for sample-position-dependence effect in photoacoustic spectrometry.

Sample position dependence effect in photoacoustic (PA) spectrometry has been reported by several scientists. This effect must be taken into account in a PA application that requires a quantitative theoretical treatment. In this work, we experimentally investigated PA signal magnitude varying with sample-to-window distance in an MTEC Model 300 Photoacoustic Detector, which has a fixed empty (gas) volume in addition to the sample-to-window-distance-dependent gas volume.