Comprehensive dataset of interactors for the entire PARP family using TurboID proximity labeling.

A comprehensive dataset detailing protein interactors for the PARP family has been generated using TurboID proximity labeling under standardized experimental conditions. V5-TurboID fusion constructs enabled identification of 6,314 high-confidence interacting proteins through mass spectrometry, capturing transient interactions undetectable by conventional methods. Parallel GFP-PARP localization experiments validated physiological subcellular distributions.

Detection method of subgrade settlement for the road of ART in coastal tidal flat area based on Vehicle-mounted binocular stereo vision technology.

To address the problem present in current subgrade settlement detection methods, this paper proposes a nondestructive intelligent and dynamic detection method for subgrade settlement based on vehicle-mounted binocular stereo vision technology. This method aims to achieve all season, the whole road, long-term detection of subgrade settlement for Road of ART (Autonomous rail Rapid Transit) in coastal tidal flat areas. Firstly, improved Schneider encoding is adopted as the marker for subgrade settlement monitoring points.

Oncogenic and microenvironmental signals drive cell type specific apoptosis resistance in juvenile myelomonocytic leukemia.

Juvenile myelomonocytic leukemia (JMML) is caused by constitutively activated RAS signaling and characterized by increased proliferation and predominant myelomonocytic differentiation of hematopoietic cells. Using MxCre;Ptpn11 mice, which model human JMML, we show that RAS pathway activation affects apoptosis signaling through cell type-dependent regulation of BCL-2 family members. Apoptosis resistance observed in monocytes and granulocytes was mediated by overexpression of the anti-apoptotic and down-regulation of the pro-apoptotic members of the BCL-2 family.

Power-free knee rehabilitation robot for home-based isokinetic training.

Robot-assisted isokinetic training has been widely adopted for knee rehabilitation. However, existing rehabilitation facilities are often heavy, bulky, and extremely energy-consuming, which limits the rehabilitation opportunities only at designated hospitals. In this study, we introduce a highly integrated and lightweight (52 kg) knee rehabilitation robot that can provide home-based isokinetic training without external power.

Observed different impacts of potential tree restoration on local surface and air temperature.

Tree restoration can cool or warm the local climate through biophysical processes. However, the magnitude of these effects remains unconstrained at large scales, as most previous observational studies rely on land surface temperature (Ts) rather than the more policy-relevant air temperature (Ta). Using satellite observations, we show that Ta responds to tree cover change at only 15-30% of the magnitude observed in Ts.

Multi-gene metabolic engineering of Pichia pastoris to synthesize ectoine.

As a promising osmolyte, ectoine has been widely applied in cosmetics, food, and pharmaceutical industries in recent years, therefore its biomanufacturer has attracted increasing interest. Ectoine-producing isolates were previously screened from halophilic microorganisms. After ectoine synthetase was identified, genetic engineering of Escherichia coli, Corynebacterium glutamicum, and Hansenula polymorpha were employed to produce ectoine.

Liver transplantation for hepatocellular carcinoma: differences in pre-transplant radiology versus explant pathology and impact on survival.

Background: Suitability for liver transplantation in patients with hepatocellular carcinoma (HCC) is based on Milan imaging criteria developed several decades ago. We sought to compare pre-transplant imaging with explant liver histopathology in a national retrospective observational study.

Methods: All patients who underwent liver transplantation for HCC at the Scottish Liver Transplant Unit (2015-2020) were included.

Ivonescimab versus pembrolizumab for PD-L1-positive non-small cell lung cancer (HARMONi-2): a randomised, double-blind, phase 3 study in China.

Background: Ivonescimab is a bispecific antibody against programmed cell death protein 1 and vascular endothelial growth factor, yielding promising clinical outcomes for patients with advanced non-small cell lung cancer in early-phase studies. We compared the efficacy and safety of ivonescimab with pembrolizumab in patients with programmed cell death ligand-1 (PD-L1)-positive advanced non-small cell lung cancer.

Methods: HARMONi-2 is a randomised, double-blind, phase 3 trial across 55 hospitals in China.

Dielectric breakdown-based ambient ionization mass spectrometry assisted by a cotton swab wrapped with aluminum foil.

Background: Ambient ionization mass spectrometry (MS) is widely used due to its simplicity. Dielectric breakdown-based ionization has emerged as a promising technique, where air molecules like nitrogen are ionized, producing N and electrons. These initiate ion-molecule reactions that generate protons from water molecules, leading to analyte ionization via protonation.

Hydrodynamic cavitation synthesis of water-soluble CdSe quantum dots: Effect of venturi tube parameters on size distribution and fluorescence properties, with application in Fe detection.

Background: Fe is an important trace element in life metabolism and also the main component of industrial pollutant. How to improve the sensitivity of Fe detection is of great significance in biological and environmental analyses. Fluorescence probe for Fe detection has high sensitivity, but it is difficult to prepare a probe with high sensitivity.

Determination of 70 liquid crystal monomers in indoor gas-particle-dust multi-medium based on ultrasonic extraction coupled with comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry.

Liquid crystal monomers (LCMs) are a class of emerging organic pollutants widely used in liquid crystal displays (LCDs) of various electronic products. LCMs can easily be released from LCDs into indoor environments and may pose potential environmental and health risks. Meanwhile, LCMs exhibit a wide range of saturation vapor pressures (PL) and octanol-air partition coefficients (Koa), which enable their presence in various indoor metrices.

ACA-24-3995.Highlighted: Determination of lopinavir and ritonavir in hERG solution to support in vitro hERG block potency assessment using LC-MS/MS: The challenge of poor drug solubility.

Background: ICH-S7B recommends assessing hERG channel block using patch clamp method to understand the risk of clinical QT prolongation and the associated rare but potentially fatal ventricular tachyarrhythmia, Torsade de Pointes. Drug concentrations exposed to the cells during hERG assays may differ from the intended concentrations due to either human errors or drug properties leading to drug loss. Concentration verification is challenging for drugs with limited solubility, samples may contain solutes and insoluble particles that may solubilize during sample analysis and resulting in inaccurate measurements of cell exposure.

FeO@Au magnetic SERS nanotags-based vertical flow immunoassay for simultaneous detection of fumonisin B1, aflatoxin B1 and deoxnivalenol.

Agricultural products are usually contaminated by various mycotoxins, the rapid and sensitive detection of mycotoxins at the early stage is significant for food safety and human health. Here, we develop a rapid and ultrasensitive surface-enhanced Raman scattering-based vertical flow assay (VFA) for simultaneous detection of multiple mycotoxins by using FeO@Au magnetic SERS nanotags. In this competitive immunoassay, three test zones are designed on the sensing membrane, and modified with multiple capture antigens.

Development of a multichannel hand-adaptive tactile stimulation device for somatotopic map of human hand in somatosensory cortex with fMRI.

The 7T functional magnetic resonance imaging (fMRI) can provide a detailed somatotopic map. However, due to the constraints of MR-compatible applications, current tactile stimulation devices for the human hand are insufficient for precise somatotopic mapping experiments. In this study, we developed a novel 23-channel, hand-adaptive tactile stimulation device with high temporal and spatial resolution.

HBCOC attenuates cerebral ischemia-reperfusion injury in mice by inhibiting the inflammatory response and autophagy via TREM-1/ERK/NF-κB.

Objective: Hemoglobin-based carbon monoxide carrier (HBCOC) can dissociate carbon monoxide and ameliorate organ damage by inhibiting inflammation and oxidative stress. In this study, we evaluated its effect on cerebral ischemia-reperfusion injury in mice and explored its potential mechanism.

Methods: A middle cerebral artery occlusion/reperfusion (MCAO/R) mouse model was established using the wire embolization method, and HBCOC or equivalent normal saline was administered via the tail vein during reperfusion.

Factors influencing occupational burnout among palliative care practitioners in China.

Objective: The aim of this investigation was to examine the job burnout status among health practitioners in palliative care wards within a specific province of China and to analyze associated factors.

Methods: A multicenter cross-sectional study design was utilized from August to October 2023,enrolling 164 health practitioners from 9 hospitals within the palliative care wards of the specified province. Data collection involved anonymous questionnaires covering basic personal information, job characteristics, and levels of occupational burnout.

The path winds along isolation and analyses of fetal nucleated red blood cells in maternal peripheral blood: Past, present, and future toward non-invasive prenatal diagnosis.

Traditional prenatal diagnosis detects fetal disorders through invading uterus to access fetal cells, which may cause maternal complications, fetal injury, and even miscarriage. Safe and convenient non-invasive prenatal testing (NIPT) by analyzing fetal materials (cell-free DNA/RNA, cells, and extracellular vesicles) that circulate in maternal peripheral blood attracts great attention and has been applied in risk evaluation of several fetal disorders. Among those fetal analytes, fetal nucleated red blood cells (fNRBCs) comprise entire fetal genome, possess distinct membrane antigens, and have a lifespan limited in every single gestation.

Direction of TIS envelope electric field: Perpendicular to the longitudinal axis of the hippocampus.

Background: Temporal Interference Stimulation (TIS) is a non-invasive approach to deep brain stimulation. However, most research has focused on the intensity of modulation, with limited attention given to the directional properties of the induced electric fields, despite their potential importance for precise stimulation.

New Methods: A novel analytical framework was developed to analyze TIS-induced electric field directions using individual imaging data.

NADPH oxidase 2 inhibitor GSK2795039 prevents against cardiac remodeling after MI through reducing oxidative stress and mitochondrial dysfunction.

Myocardial infarction (MI) is the leading cause of mortality in cardiovascular diseases and continues to pose a substantial challenge in clinical management, despite the availability of guideline-directed medical therapy. The present study aimed to investigate the potential protective effects of the NADPH oxidase inhibitor (NI) GSK2795039 on cardiac remodeling following MI, and to elucidate the underlying mechanisms involved. We established the MI model by ligating the left anterior descending artery in mice.

Cath-HG exhibits anti-acne activities via multiple mechanisms.

The overgrowth of Propionibacterium acnes and the subsequent inflammation possess critical roles during acne vulgaris development. Cath-HG, an antimicrobial peptide previously characterized for its antimicrobial, anti-inflammatory, human protease-regulating, LPS-binding, and platelet glycoprotein VI (GPVI) inhibitory properties, has not been studied in the context of acne vulgaris. Here, Cath-HG was found to show strong anti-bacterial activities against P.

Impact of sodium butyrate on stroke-related intestinal injury in diabetic mice: interference with Caspase-1/GSDMD pyroptosis pathway and preservation of intestinal barrier.

Diabetic stroke-associated acute intestinal injury is characterized by high mortality, disability, and poor prognosis due to the lack of effective therapies. Our prior research demonstrated that administration of 300 mg/kg sodium butyrate (NaB) can improve neurological outcomes post-diabetic stroke. Nonetheless, whether the effect of NaB is related to intestinal regulation, along with its underlying mechanisms, remains uncertain.

Blood Pressure and Heart Rate Management in Patients After Repair of Type A Aortic Dissection.

Background: The long-term optimal targets for blood pressure and heart rate in patients post-surgical repair of acute type A aortic dissection (TAAD) remain unclear. This study aimed to evaluate the long-term association of blood pressure and heart rate control on patients post-TAAD surgical repair.

Methods: We identified 839 patients who underwent TAAD surgical repair and survived to discharge between 2003 and 2018 from the Chang Gung research database.