Proteogenomic characterization reveals tumorigenesis and progression of lung cancer manifested as subsolid nodules.

Lung adenocarcinoma (LUAD) radiologically displayed as subsolid nodules (SSNs) is prevalent. Nevertheless, the precise clinical management of SSNs necessitates a profound understanding of their tumorigenesis and progression. Here, we analyze 66 LUAD displayed as SSNs covering 3 histological stages including adenocarcinoma in situ (AIS), minimally invasive adenocarcinoma (MIA) and invasive adenocarcinoma (IAC) by incorporating genomics, proteomics, phosphoproteomics and glycoproteomics.

Self-assembly of short peptides activates specific ER-phagy and induces pyroptosis for enhanced tumor immunotherapy.

Developing specific endoplasmic reticulum-autophagy (ER-phagy) inducers is highly desirable for discovering new ER-phagy receptors, elucidating the detailed ER-phagy mechanism and potential cancer immunotherapy. However, most of the current ER-phagy-inducing methods cause non-selective autophagy of other organelles. In this work, we report the design and synthesis of simple and stable short peptides (D-FFxFFs) that could specifically trigger ER-phagy, which further induces pyroptosis and activates immune response against tumor cells.

Chemical Probe-Enabled Lipid Droplet Proteomics.

Emerging evidence indicates that lipid droplets (LDs) play important roles in lipid metabolism, energy homeostasis, and cell stress management. Notably, dysregulation of LDs is tightly linked to numerous diseases, including lipodystrophies, cancer, obesity, atherosclerosis, and others. The pivotal physiological roles of LDs have led to an exploration of research in recent years.

Ligand Modulation in Metal-Organic Frameworks Derived Regenerable Oxygen Evolution Electrocatalysts.

Metal-organic frameworks (MOFs) are emerging as promising pre-catalysts for the oxygen evolution reaction (OER) in water electrolysis. However, the coordination chemistry of MOF ligands in reconstructed species remains poorly understood, particularly regarding how ligand modulation influences the electronic configurations of catalytic sites. In this study, we present the synthesis of an α-FeOOH coated Ni-catecholate MOF composite (FeOOH@Ni-CAT), which transforms into a ligand-coordinated γ-NiFeOOH active species during OER.

Effect of intensive water-salt diet nursing intervention on blood pressure and volume load in patients with chronic renal failure.

Background: This study aimed to investigate the impact of a comprehensive nursing intervention targeting high water and salt intake on blood pressure and volume burden in patients with chronic renal failure.

Method: From January 2020 to January 2023, 120 patients diagnosed with chronic renal failure were treated at our hospital. Participants were randomly allocated to either a control group ( = 60) receiving standard dietary education or an observation group ( = 60) receiving intensive water-salt diet nursing intervention alongside standard education.

Comparison of continuous versus on-demand proton pump inhibitor therapy in symptom control of patients with Barrett's esophagus.

Background: Whether continuous proton pump inhibitor (PPI) therapy is superior to on-demand therapy for symptom control of Barrett's esophagus patients is unclear. The study aimed to compare the efficacies of the symptom control and the frequency of co-existent erosive esophagitis in patients with Barrett's esophagus by either continuous or on-demand PPI therapy.

Methods: We randomly assigned (1:1) consecutive adult patients with symptomatic Barrett's esophagus to receive on-demand or continuous esomeprazole (40 mg q.

BRACO-19 targeted the G-quadruplex in the 3'UTR of the Cucumber mosaic virus 1a, 2b, and CP genes to inhibit viral proliferation.

Background: The development of highly efficient and environmentally friendly plant virus inhibitors with novel structures mostly depends on the discovery of new targets. G-quadruplexes are non-classical high-level structures that are formed by guanine-rich nucleic acid sequences, which have significant biological functions. Small molecules that target the G-quadruplexes in the genomes of animal viruses have been shown to inhibit viral proliferation.

Identification and functional characterization of silk fibroin genes in leaf-folding behaviour, silk properties and development of the rice leaf folder, Cnaphalocrocis medinalis (lepidoptera: Pyralidae).

Background: Outbreaks of the rice leaf folder (Cnaphalocrocis medinalis) poses a significant threat to rice production. Silk-spinning creates an enclosed leaf roll that protects larvae from predators and insecticides, while serving as a feeding site and facilitating host transfer and leaf-folding. Disrupting this behaviour could enhance control strategies.

Enhancing single-cell classification accuracy using image conversion and deep learning.

Single-cell transcriptome sequencing (scRNA-seq) is widely used in the fields of animal and plant developmental biology and important trait analysis by obtaining single-cell transcript abundance data in high throughput, which can deeply reveal cell types, subtype composition, specific gene markers and functional differences. However, scRNA-seq data are often accompanied by problems such as high noise, high dimensionality and batch effect, resulting in a large number of low-expressed genes and variants, which seriously affect the accuracy and reliability of data analysis. This not only increases the complexity of data processing, but also limits the effectiveness of feature selection and downstream analysis.

Molecular mechanism of Mfn2 alleviating endoplasmic reticulum stress and inhibiting apoptosis of sheep follicular granulosa cells.

Follicle development is a crucial step in mammalian reproductive processes, the specific role of Mfn2 in regulating mitochondrial function and endoplasmic reticulum stress in this process is still unclear, this study aimed to investigate the role of Mfn2 in the follicular development of adult sheep. Large, medium, and small follicles were collected, and granulosa cells (GCs) were isolated from large follicles. The expression levels of Mfn2 in different follicles were detected using qRT-PCR and Western blot, and the localization of Mfn2 in follicles was determined through immunofluorescence.

Expert consensus on clinical genome sequencing interpretation and reporting.

Genome sequencing (GS) refers to a technology that comprehensively and systematically detects the DNA sequences of an individual's nuclear and mitochondrial genomes. It aims to identify genetic variants and investigate their roles in human health and disease progression. As an emerging diagnostic tool, GS offers significant support for clinical diagnosis due to its high throughput, accuracy, and comprehensiveness.

A Uniform Solid Electrolyte Interface Enabling Long Cycling in Sodium-Metal Batteries with Fast Charging.

The high reactivity of sodium leads to significant safety challenges, while the unstable solid electrolyte interphase (SEI) further complicates its use in sodium-metal batteries (SMBs), collectively impeding their path to commercialization. A deep eutectic electrolyte (DEE) is introduced, which addresses these challenges by balancing high ionic conductivity with stable SEI formation. The introduction of -methylacetamide enhances the nonflammability of the solvent and adjusts the SEI composition.

Alpha-frequency stimulation strengthens coupling between temporal fluctuations in alpha oscillation power and default mode network connectivity.

Alpha (8-12 Hz) oscillations and default mode network (DMN) activity dominate the brain's intrinsic activity in the temporal and spatial domains, respectively. They are thought to play crucial roles in the spatiotemporal organization of the complex brain system. Relatedly, both have been implicated, often concurrently, in diverse neuropsychiatric disorders, with accruing electroencephalogram/magnetoencephalogram (EEG/MEG) and functional magnetic resonance imaging (fMRI) data linking these two neural activities both at rest and during key cognitive operations.

Bi-functional transcription factor SlbHLH95 regulates fruits flavonoid metabolism and grey mould resistance in tomato.

Flavonoids are polyphenolic secondary metabolites in tomato fruit with important roles in nutritional quality. Dissecting the transcriptional regulatory network modulating flavonoid metabolism is the first step to improve the nutritional quality of tomato fruits through molecular breeding technology. In this study, we identified a transcription factor SlbHLH95 as a key regulator in flavonoid metabolism through analysis of the MicroTom Metabolic Network (MMN) data set.

scMDCL: A Deep Collaborative Contrastive Learning Framework for Matched Single-Cell Multiomics Data Clustering.

Single-cell multiomics clustering integrates multiple omics data to analyze cellular heterogeneity and is crucial for uncovering complex biological processes and disease mechanisms. However, existing matched single-cell multiomics clustering methods often neglect the full utilization of intercellular relationships and the interactions and synergy between features from different omics, leading to suboptimal clustering performance. In this paper, we propose a deep collaborative contrastive learning framework for matched single-cell multiomics data clustering, named scMDCL.

Conservative Management of Macroglossia in Beckwith-Wiedemann Syndrome.

Introduction: While severe macroglossia in Beckwith-Wiedemann syndrome (BWS) is often treated with tongue reduction surgery, most patients with mild to moderate macroglossia are nonsurgically managed. Given that outcomes for these conservatively managed patients are not well characterized, this study aims to describe the natural history of patients with macroglossia and BWS who did not undergo early surgery.

Methods: Records of patients with BWS and macroglossia seen between 2004-2024 were reviewed.

Column Chart Prediction Model for Ovarian Cancer Based on Serum Ovarian Tumor Related Biomarkers and Validation.

Purpose: The aim was to study the predictive model and validate serum ovarian tumor-related biomarkers for ovarian cancer histograms.

Method: We randomly selected 181 patients with ovarian tumors and 80 healthy individuals who underwent physical examinations from the hospital's medical record information system as the study participants. Clinical data and detection results of ovarian tumor-related markers such as serum carcinoembryonic antigen (CEA), carbohydrate antigen 125 (CA125), carbohydrate antigen 19-9 (CA19-9), and human epididymal protein (HE4) were collected from all study participants for analysis.

The real-world safety profile of sertraline: Pharmacovigilance analysis of the FAERS database.

Objective: Sertraline is a widely used antidepressant, and its safety characteristics in elderly and adolescent patients have not been fully clarified. The purpose of this study was to comprehensively evaluate the characteristics of adverse effects of sertraline through a large-scale analysis of real-world data and to add to the evidence in the elderly and adolescent populations.

Methods: The data were extracted from the Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS) database from the first quarter (Q1) 2004 to Q1 2024 for data cleaning and analysis.

Liver stiffness measurement trajectory analysis for prognosis in patients with chronic hepatitis B and compensated advanced chronic liver disease.

Introduction And Objectives: Liver stiffness measurements (LSMs) offer a noninvasive method for monitoring liver disease development. This study evaluated the prognostic value of different LSM trajectories in chronic hepatitis B (CHB) and compensated advanced chronic liver disease (cACLD) patients.

Materials And Methods: We retrospectively analyzed 1272 CHB and cACLD patients with at least two LSMs, applied group-based trajectory modeling (GBTM) to identify distinct LSM trajectories, and used a Cox model to analyze their associations with liver-related events (LREs) and mortality risk.

An ethylene response factor negatively regulates red light induced resistance of melon to powdery mildew by inhibiting ethylene biosynthesis.

Powdery mildew is a common serious disease threatening global melon production. Red light can improve plant resistance to powdery mildew by inducing endogenous ethylene synthesis; however, the underlying molecular mechanism requires elucidation. In this study, an ERF transcription factor CmRAP2-13 was identified, silencing it significantly improved melon seedlings resistance to powdery mildew.

Structural characterization of a pectin-like polysaccharide from Clematis chinensis Osbeck and its anti-rheumatoid arthritis activity.

Clematis chinensis Osbeck is a traditional Chinese medicine to treat rheumatic arthritis (RA). We hypothesized that C. chinensis polysaccharide, as a bioactive ingredient, might have the anti-RA function.

Wearable transdermal drug delivery system controlled by wirelessly powered acoustic waves.

Transdermal drug administration offers an alternative route for drug delivery through the skin, and surface acoustic wave (SAW) technology has recently emerged as a promising approach to enhance this process. However, conventional cable-connected SAW control units face several challenges, including inconvenience, poor wearability, limited miniaturization and integration, and restricted reusability. This study introduces a wireless-powered transport strategy for the transdermal delivery of large drug molecules using a thin-film-based SAW platform.

Development and validation of radiomics and deep transfer learning models to assess cognitive impairment in patients with cerebral small vessel disease.

Cognitive impairment in cerebral small vessel disease (CSVD) progresses subtly but carries significant clinical consequences, necessitating effective diagnostic tools. This study developed and validated predictive models for CSVD-related cognitive impairment using deep transfer learning (DTL) and radiomics features extracted from hippocampal 3D T1-weighted MRI. A total of 145 CSVD patients and 99 control subjects were enrolled in the study.