Publications by authors named "Jianpeng Ao"

Numerous chemical reactions and most life processes occur in aqueous solutions, where the physical diffusion of small molecules plays a vital role, including solvent water molecules, solute biomolecules, and ions. Conventional methods of measuring diffusion coefficients are often limited by technical complexity, large sample consumption, or significant time cost. Here, we present an optical imaging method to study molecular diffusion by combining stimulated Raman scattering (SRS) microscopy with microfluidics: a "Y"-shaped microfluidic channel forming two laminar flows with a stable concentration gradient across the interface.

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  • * Stimulated Raman scattering (SRS) microscopy is used to observe the metabolic activities of deuterated water in Arabidopsis thaliana, showing how it forms C-D bonds in new biomolecules.
  • * The study reveals spatial differences in metabolic activities within plant structures and tracks the movement of protons from plants to aphids, highlighting SRS microscopy's capability to study various matter flows in plants like carbon storage and nutrition.
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People of all ages consume salt every day, but is it really just salt? Plastic nanoparticles [nanoplastics (NPs)] pose an increasing environmental threat and have begun to contaminate everyday salt in consumer goods. Herein, we developed a combined surface enhanced Raman scattering (SERS) and stimulated Raman scattering (SRS) approach that can realize the filtration, enrichment, and detection of NPs in commercial salt. The Au-loaded (50 nm) anodic alumina oxide substrate was used as the SERS substrate to explore the potential types of NP contaminants in salts.

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  • Intraoperative histology is critical for surgical decisions, but traditional frozen-section H&E staining lacks accuracy and takes too long compared to the gold-standard FFPE method.
  • Stimulated Raman scattering (SRS) microscopy provides quick imaging of brain tissue, but transforming these images to look like conventional H&E stains for accurate interpretation by pathologists is a challenge.
  • A newly developed semi-supervised CycleGAN model allows for rapid conversion of SRS images to H&E stains within 3 minutes, achieving high accuracy that may enhance intraoperative diagnosis of gliomas and potentially other tumors.
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Histological examination is crucial for cancer diagnosis, however, the labor-intensive sample preparation involved in the histology impedes the speed of diagnosis. Recently developed two-color stimulated Raman histology could bypass the complex tissue processing to generates result close to hematoxylin and eosin staining, which is one of the golden standards in cancer histology. Yet, the underlying chemical features are not revealed in two-color stimulated Raman histology, compromising the effectiveness of prognostic stratification.

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The efficient elimination of per- and polyfluoroalkyl substances (PFASs) from the environment remains a huge challenge and requires advanced technologies. Herein, we demonstrate that perfluorooctanoic acid (PFOA) photochemical decomposition could be significantly accelerated by simply carrying out this process in microdroplets. The almost complete removal of 100 and 500 μg/L PFOA was observed after 20 min of irradiation in microdroplets, while this was achieved after about 2 h in the corresponding bulk phase counterpart.

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  • Mesenchymal stem cells (MSCs) are vital for tissue engineering as their differentiation affects the quality of cultured tissue, crucial for successful transplantation.
  • Precise control of MSC differentiation is necessary to prevent issues like tumor formation in stem cell therapy.
  • Researchers used advanced imaging techniques and a K-means machine learning model to automate the evaluation of MSC differentiation, enhancing analysis and potential applications in stem cell research.
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Aerosol microdroplets as microreactors for many important atmospheric reactions are ubiquitous in the atmosphere. pH largely regulates the chemical processes within them; however, how pH and chemical species spatially distribute within an atmospheric microdroplet is still under intense debate. The challenge is to measure pH distribution within a tiny volume without affecting the chemical species distribution.

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Core-needle biopsy (CNB) plays a vital role in the initial diagnosis of breast cancer. However, the complex tissue processing and global shortage of pathologists have hindered traditional histopathology from timely diagnosis on fresh biopsies. In this work, we developed a full digital platform by integrating label-free stimulated Raman scattering (SRS) microscopy with weakly-supervised learning for rapid and automated cancer diagnosis on un-labelled breast CNB.

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Fe(III)-oxalate complexes are ubiquitous in atmospheric environments, which can release reactive oxygen species (ROS) such as HO, O, and OH under light irradiation. Although Fe(III)-oxalate photochemistry has been investigated extensively, the understanding of its involvement in authentic atmospheric environments such as aerosol droplets is far from enough, since the current available knowledge has mainly been obtained in bulk-phase studies. Here, we find that the production of OH by Fe(III)-oxalate in aerosol microdroplets is about 10-fold greater than that of its bulk-phase counterpart.

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Unlabelled: Focal therapy (FT) has been proposed as an approach to eradicate clinically significant prostate cancer while preserving the normal surrounding tissues to minimize treatment-related toxicity. Rapid histology of core needle biopsies is essential to ensure the precise FT for localized lesions and to determine tumor grades. However, it is difficult to achieve both high accuracy and speed with currently available histopathology methods.

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Gastroscopic biopsy provides the only effective method for gastric cancer diagnosis, but the gold standard histopathology is time-consuming and incompatible with gastroscopy. Conventional stimulated Raman scattering (SRS) microscopy has shown promise in label-free diagnosis on human tissues, yet it requires the tuning of picosecond lasers to achieve chemical specificity at the cost of time and complexity. Here, we demonstrate that single-shot femtosecond SRS (femto-SRS) reaches the maximum speed and sensitivity with preserved chemical resolution by integrating with U-Net.

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We report the generation of parabolic pulses with broadband spectrum from a core-pumped Yb-doped fiber amplifier seeded by a dispersion managed fiber oscillator. The net cavity dispersion of Yb-doped oscillator was continuously changed from 0.074 to -0.

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Photochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman probes with stimulated Raman scattering (SRS) microscopy provides superb chemical resolution for super-multiplexed imaging, but are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging.

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Gout is a common metabolic disease with growing burden, caused by monosodium urate (MSU) microcrystal deposition. In situ and chemical-specific histological identification of MSU is crucial in the diagnosis and management of gout, yet it remains inaccessible for current histological methods. Stimulated Raman scattering (SRS) microscopy was utilized to image MSU based on its fingerprint Raman spectra.

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We have proposed and implemented a polarization-maintaining passively synchronized fiber laser system, which could deliver tunable dual-color picosecond pulses by including a frequency-doubling module and a spectral broadening module. Specifically, the output from the involved Er-doped fiber laser were used to generate second-harmonic pulses at 790 nm with a quadratic nonlinear crystal. In parallel, the amplified pulses from the synchronized Yb-doped fiber laser were launched into a 150-m single mode fiber, which resulted in not only substantial spectral bandwidth broadening from 0.

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