Nerve Growth Factor from Pancreatic Cancer Cells Promotes the Cancer Progression by Inducing Nerve Cell-Secreted Interleukin-6.

Pancreatic cancer (PC) is a cancer with a poor prognosis, and nerve growth factor (NGF) is involved in the pathogenesis of PC within the unknown exact role. Herein, SW1990 cells and PC12 cells were co-cultured using transwell co-culture system and subsequently revealed that NGF was overexpressed in SW1990 cells and promoted PC12 cell proliferation. Knockdown of NGF expression in SW1990 cells using lentiviral shRNA effectively inhibited NGF expression in SW1990 cells and reduced its stimulatory effect on PC12 cell proliferation.

Research on laser target dynamic tracking system with rotating polarization grating.

A micromechanical target tracking system based on polarization grating (PG) is designed to meet the conformal design of laser communication systems and to realize the lightweight and miniaturization of space laser communication networking. The rotating dual PGs are applied to the dynamic tracking of laser targets for the first time, the relationship between the target position and the dual polarization gratings (PGs) angles is defined, and the PG beam deflection multi-coordinate construction and decoupling are carried out. A dual PGs mathematical model was established, and a controller based on the dual PGs system loop was designed.

A novel germline frameshift mutation in the MLH1 gene in a patient with Lynch syndrome.

Lynch syndrome (LS) is an autosomal dominant inherited disorder, characterized by a predisposition to various cancers, mainly colorectal cancer (CRC). LS is caused by germline mutations in DNA mismatch repair genes i.e.

A combination of methylation and protein markers is capable of detecting gastric cancer detection by combined markers.

This study aimed to validate a combination of , and CA724 for gastric cancer (GC) detection. The performance of , and CA724 was examined in a prospective cohort study with 518 participants (151 with GC, 56 with atrophic gastritis, 87 with other gastrointestinal diseases and 224 with no evidence of disease). , or CA724 alone detected 48.

The enhancement of Tetrandrine to gemcitabine-resistant PANC-1 cytochemical sensitivity involves the promotion of PI3K/Akt/mTOR-mediated apoptosis and AMPK-regulated autophagy.

Background: In the process of tumor development, the resistance of pancreatic cancer cells to gemcitabine (GEM) is mainly due to the suppression and dysregulation of apoptosis signals to a large extent. Therefore, it is very necessary to develop pro-apoptotic drugs for combined treatment of pancreatic cancer to increase the activity of GEM and improve the prognosis of pancreatic cancer.

Methods And Results: GEM-resistant PANC-1 cells were treated with increasing doses of GEM.

Controllable linear asymmetric transmission and perfect polarization conversion in a terahertz hybrid metal-graphene metasurface.

We propose a three layered metal-graphene-metal metasurface to investigate the controllable linear asymmetric transmission and perfect polarization conversion in THz regime, by using the finite-difference time-domain (FDTD) method. An on-to-off control of asymmetric transmission and perfect polarization conversion is achieved by changing the Fermi energy of graphene from 0.8 eV to 0 eV.

ProNGF siRNA inhibits cell proliferation and invasion of pancreatic cancer cells and promotes anoikis.

Background: Precursor of nerve growth factor (proNGF) was previously considered biologically inactive; however, it has recently been identified as having important roles in the pathology of cancer development.

Aim: This study aimed to explore the therapeutic effects of proNGF siRNA on the proliferation, invasion, and anoikis of pancreatic cancer cells and determine the functions of proNGF.

Methods: Pancreatic ductal adenocarcinoma (PDAC) and paired paracancerous tissue samples were collected from 60 patients for evaluation of proNGF expression by immunohistochemistry staining, qPCR, and western blotting.

RNA interference‑mediated inhibition of survivin and VEGF in pancreatic cancer cells in vitro.

The aim of the present study was to investigate the effects of simultaneous short hairpin RNA (shRNA)‑targeted survivin and vascular endothelial growth factor (VEGF) inhibition on the proliferation, apoptosis and angiogenesis of human pancreatic cancer cells (Panc‑1). Targeted small interfering RNA (siRNA) expression vectors of survivin and VEGF were constructed and transfected into Panc‑1 cells. The downregulation of survivin and VEGF expression was evaluated by real‑time PCR and western blot analysis.

Double inhibition of NF-κB and XIAP via RNAi enhances the sensitivity of pancreatic cancer cells to gemcitabine.

The majority of patients with pancreatic cancer are resistant to gemcitabine. One of the mechanisms involved is the anti-apoptotic ability of these cells. The median lethal dose (LD50) of gemcitabine for PANC-1 cells was higher than that for Mia PaCa-2 cells and the former had higher nuclear factor-κB (NF-κB) and X-linked inhibitor of apoptosis protein (XIAP) levels.

Genetic immune modulation of Ran GTPase against different microbial pathogens.

Septic shock characterized by pro-inflammatory cytokine storm can be induced by a variety of microbial infections. Typical pro-inflammatory cytokines include TNFalpha, IL1 and IL6. Although one or more of them is often expressed in any given microbial infection, usually it is the elevation of one cytokine that becomes predominant at a particular time in a given infection.