Generation of an induced pluripotent stem cell (iPSC) line (INNDSUi008-A) from a patient with Spinocerebellar Ataxia Type 3.

Abnormal trinucleotide CAG repeat expansions in exon 10 of the ataxin-3 (ATXN3) gene has been identified as the cause of Spinocerebellar Ataxia Type 3 (SCA3). We generated and characterized a human induced pluripotent stem cell (iPSC) line from skin fibroblasts of a patient with genetically confirmed SCA3. The pluripotency of these iPSCs was verified by the expression of several undifferentiated hPSCs markers at both RNA and protein levels, as well as their capability to differentiate into all three germ layers.

Molecular Basis of PAC1R Allosteric Modulation with Lipids in Membranes.

The pituitary adenylate cyclase-activating polypeptide receptor I (PAC1R) represents a highly sought-after therapeutic target for chronic pain, migraine, and post-traumatic stress. As a class B G protein-coupled receptor (GPCR), the PAC1R is highly expressed throughout the neuronal and central nervous system membranes, with the receptor subject to hormone activation and subsequent signal transduction. Despite its desirable indication, small molecule agonists for PAC1R have been notoriously difficult to develop due to competition with PACAP.

Deformation-Induced Multioptical Morphology Elastomer Constructed from Phosphorescent Nanospheres for Underwater Mechanical Sensing.

Combination of multioptical morphology, such as transmission, scattering, fluorescence (FL), and room-temperature phosphorescence (RTP), to build multisignal-integrated devices is highly attractive in future optical devices but extremely difficult owing to the poorly matched material design and construction principles. Here, we report a novel multioptical morphology elastomer (MOME) fabricated by encapsulating monodisperse RTP SiO nanoparticles (RTP-SiO NPs) with polydimethylsiloxane (PDMS). The switching behavior of optical signals is dependent on the deformation of MOME, such as stretching, bending, and squeezing.

Maternal intake of dairy products is inversely associated with birth weight in women with gestational diabetes mellitus: A prospective cohort study.

This study aimed to investigate the intake of dairy products during pregnancy in women with gestational diabetes mellitus (GDM), and its impacts on neonatal birth weight and pregnancy outcomes. A total of 386 women with GDM during the second trimester pregnancy participated in this prospective cohort study. We evaluated dairy products intake through Food Frequency Questionnaire.

Identifying prognostic biomarkers and immune interactions in ovarian cancer associated with perfluorooctanoic acid exposure: Insights from comparative toxicogenomics and molecular docking studies.

Background: Perfluorooctanoic acid (PFOA) exposure has been implicated in various health issues. This study aims to identify common genes associated with PFOA exposure and ovarian cancer, elucidate their biological functions, and explore their prognostic significance.

Methods: We identified common genes linked to PFOA exposure and ovarian cancer using the Comparative Toxicogenomics Database.

Covalent-Allosteric Inhibitors: Do We Get the Best of Both Worlds?

Covalent-allosteric inhibitors (CAIs) may achieve the best of both worlds: increased potency, long-lasting effects, and reduced drug resistance typical of covalent ligands, along with enhanced specificity and decreased toxicity inherent in allosteric modulators. Therefore, CAIs can be an effective strategy to transform many undruggable targets into druggable ones. However, CAIs are challenging to design.

Expression and correlation analysis of VISTA in peripheral blood mononuclear cells of myasthenia gravis patients.

Myasthenia gravis (MG) is a T cell-dependent, B cell-mediated disorder strongly associated with antigen presentation by dendritic cells (DCs). In MG, mucosal tolerance is linked to increased expression of TGF-β mRNA in monocytes. Additionally, monocytic myeloid-derived suppressor cells (M-MDSCs) exhibit negative immunomodulatory effects by suppressing autoreactive T and B cells.

Analysis of the Flavor Code of Newly Cultivated Excellent Apple Varieties in China Using the Sensomics.

"Rui Yang", "Rui Xue", and "Rui Xiang Hong" are newly cultivated apple varieties with excellent flavor. However, the key aroma-active compounds responsible for its excellent flavor quality were unclear. This study determined the characteristics of aroma compounds among three new apple varieties and their parental species by Sensomics.

Association between base excess and mortality in septic older adults.

Aim: Septic older adults represent a vulnerable population with high mortality rates. Base excess (BE), an indicator of acid-base status, may serve as a prognostic marker in sepsis. This study aimed to investigate the association between BE and mortality in septic older adults.

Single-step retrosynthesis prediction via multitask graph representation learning.

Inferring appropriate synthesis reaction (i.e., retrosynthesis) routes for newly designed molecules is vital.

N6-methyladenosine RNA modification regulates the transcription of SLC7A11 through KDM6B and GATA3 to modulate ferroptosis.

Background: Recent studies indicate that N6-methyladenosine (mA) RNA modification may regulate ferroptosis in cancer cells, while its molecular mechanisms require further investigation.

Methods: Liquid Chromatography-Tandem Mass Spectrometry (HPLC/MS/MS) was used to detect changes in mA levels in cells. Transmission electron microscopy and flow cytometry were used to detect mitochondrial reactive oxygen species (ROS).

MTGGF: A Metabolism Type-Aware Graph Generative Model for Molecular Metabolite Prediction.

Metabolism in vivo turns small molecules (e.g., drugs) into metabolites (new molecules), which brings unexpected safety issues in drug development.

Lignocellulosic biomass as promising substrate for polyhydroxyalkanoate production: Advances and perspectives.

The depletion of fossil resources, coupled with global warming and adverse environmental impact of traditional petroleum-based plastics, have necessitated the discovery of renewable resources and innovative biodegradable materials. Lignocellulosic biomass (LB) emerges as a highly promising, sustainable and eco-friendly approach for accumulating polyhydroxyalkanoate (PHA), as it completely bypasses the problem of "competition for food". This sustainable and economically efficient feedstock has the potential to lower PHA production costs and facilitate its competitive commercialization, and support the principles of circular bioeconomy.

Generation of an induced pluripotent stem cell (iPSC) line (INNDSUi007-A) from a patient with Kennedy disease.

Abnormal trinucleotide CAG repeat expansions in exon 1 of the Androgen Receptor (AR) gene has been identified as the cause of Kennedy disease (KD). We generated and characterized a human induced pluripotent stem cell (iPSC) line from peripheral blood mononuclear cells (PBMC) of a patient with genetically confirmed KD. The pluripotency of these iPSCs was verified by the expression of several pluripotency markers at both RNA and protein levels, as well as their capability to differentiate into all three germ layers.

Association of maternal dietary cholesterol intake during the second and third trimesters of pregnancy and blood glucose and pregnancy outcome in women with gestational diabetes mellitus: a prospective cohort study.

Background: Cholesterol is essential for pregnant women to maintain maternal health and fetal support development. This study aimed to assess the cholesterol intake of women with gestational diabetes mellitus (GDM) during the second and third trimesters of pregnancy and to explore its effects on blood glucose and pregnancy outcomes.

Methods: This prospective cohort study collected dietary data using a food frequency questionnaire (FFQ) administered during the 24-30 gestational weeks (first survey) and the 34-42 gestational weeks (second survey).

Structurally Stable Hollow-Fiber-Based Porous Graphene Oxide Membranes with Improved Rejection Performance by Selective Patching of Framework Defects with Metal-Organic Framework Crystals.

Graphene oxide (GO)-based membranes have demonstrated great potential in water treatment. However, microdefects in the framework of GO membranes induced by the imperfect stacking of GO nanosheets undermine their size-sieving ability and structural stability in aqueous systems. This study proposes a targeted growth approach by growing zeolitic imidazolate framework-8 (ZIF-8) nanocrystals precisely to patch microdefects as well as to cross-link the porous graphene oxide (PGO) flakes coated on the outer surface of the hollow fiber (HF) alumina substrate (named the hybrid PGO/ZIF-8 membrane).

Fatty acid traits mediate the effects of uric acid on cancers: a Mendelian randomization study.

Introduction: Previous findings on the association between uric acid (UA) levels and cancer risk are conflicting. Moreover, the mechanisms underlying the interactions between UA levels, fatty acid traits, and cancer outcomes remain complex; it is still unclear whether elevated UA levels influence fatty acid traits and, thereby, contribute to an increased cancer risk. Therefore, we aimed to investigate the association between UA levels and cancer risk, with a specific focus on the potential mediating role of fatty acid traits.

The association between stress hyperglycemia ratio and nonalcoholic fatty liver disease among U.S. adults: A population-based study.

Background And Aim: The stress hyperglycemia ratio (SHR) offers a more nuanced understanding of glucose metabolism by factoring in the background glycemia through the component of Hemoglobin A1c. The association of SHR with cardiovascular and cerebrovascular diseases has been established, but the relationship between SHR and the risk of nonalcoholic fatty liver disease (NAFLD) remains unexplored. This study aimed to elucidate the relationship between the two among U.

Visible-Light-Induced Divergent Oxygenation of Methylbenzene Utilizing Aryl Halides.

The selective oxidation of methylbenzene to value-added products is of indisputable importance in organic synthesis. Although photocatalytic oxidation reactions of toluene have achieved great success for the preparation of its oxidative products, such as carboxylic acids, benzaldehyde, and benzoate, there remains a lack of a unified photocatalytic system for the selective preparation of these oxidation products. Herein, we report a metal- and additive-free photocatalytic protocol enabled by aryl halides using O as a green oxidant for the selective synthesis of the above-mentioned three oxidation products by adjusting the reaction solvent.

Aroma Identification and Traceability of the Core Sub-Producing Area in the Helan Mountain Eastern Foothills Using Two-Dimensional Gas Chromatography and Time-of-Flight Mass Spectrometry and Chemometrics.

The combination of volatile compounds endows wines with unique aromatic characteristics and is closely related to their geographical origins. In the pursuit of origin identification and the subdivision of homogeneous production areas, clarifying the characteristics of production areas is of great significance for improving wine quality and commercial value. In this study, GC×GC-TOFMS technology was used to analyze the aroma characteristics of "Cabernet Sauvignon" wines from 26 wineries in the Helan (HL), Yinchuan (YC), Yongning (YN), Qingtongxia (QTX), and Hongsibu (HSP) sub-producing areas in the eastern foothills of Helan Mountain in Ningxia, China.