Effect of food loop and duodenal stump fixation on clinical outcomes in patients undergoing radical gastrectomy with Roux-en-Y reconstruction for distal gastric cancer: a single-center propensity score matching analysis.

Objective: This study aimed to evaluate the safety and reliability of an innovative technique-fixation of the food loop and duodenal stump-by investigating its impact on postoperative complications and overall survival (OS) in patients undergoing distal gastrectomy with Roux-en-Y reconstruction.

Methods: A retrospective analysis was conducted on patients who underwent radical distal gastrectomy and Roux-en-Y reconstruction at Suzhou Ninth People's Hospital between January 2012 and December 2023. Patients were divided into with duodenum fixation and without duodenum fixation groups based on whether food loop and duodenal stump fixation was performed during surgery.

CD39 Contributes to the Ability of Cell Invasion in Heterogeneity of Colorectal Cancer.

Tumour heterogeneity will accumulate and amplify during cell culture and passage, even if derived from the same strain. In the current study, multiple batches of colorectal cancer HCT116 and HT29 cell lines were obtained using different conditions of trypsin digestion and processed for RNA sequencing. CD39 was identified as a biomarker highly expressed in easily trypsin-digested cells compared to the undigestible ones.