Spatially distributed low-cross talk vector beams.

A spatially distributed low-cross talk vector beam refers to a vector beam that exhibits different intensities, phases, and polarization states along the propagation direction. This type of vector beam features low-cross talk between beams on different planes and finds extensive applications in optical communications and related fields. However, current technologies face challenges such as intensity interference at different imaging planes and difficulties in the precise control of phases and polarization states, which affect beam quality.

Microparticle sorting with a virtual optical chip.

We proposed an automatic sorting method based on a virtual optical chip, which was formed by a complex-amplitude beam shaping system. The automatic sorting of different micro-particles was realized by the optical forces of the intensity and phase gradients of the reconstructed optical beam. The method was verified with theoretical analysis and experimental results.

[Characterization of mutational pattern of patients with core-binding factor acute myeloid leukemia].

Objective: To characterize the mutational profile of patients with core-binding factor acute myeloid leukemia (CBF-AML).

Methods: A total of 81 acute myeloid leukemia patients were recruited, which included 36 cases of CBF-AML and 45 cases of cytogenetically normal acute myeloid leukemia (CN-AML) . Mutations of FLT3-ITD, FLT3-TKD, NPM1, c-KIT, NRAS, KRAS, TET2, IDH1/2, RUNX1, DNMT3A, GATA2, ASjXL1, TP53, PTPN11, JAK2V617F, SETBP1 and CEBPA genes were simultaneously detected by DNA-based PCR and Sanger sequencing.

[Characterizing the molecular cytogenetics in acute monocytic leukemia].

Objective: To characterize the molecular genetics of 81 patients with acute monocytic leukemia (AML).

Methods: Fluorescence in situ hybridization (FISH) was employed to detect MLL gene rearrangements. Combined mutations of 17 genes were detected by DNA-based PCR and Sanger sequencing.

[Study of NK cells dysfunction in multiple myeloma patients].

Objective: To explore the mechanism of NK cell dysfunction in patients with multiple myeloma (MM).

Methods: The expression of inhibitory receptors (CD158a and CD158b) and activating receptors NKG2D and NCRs (NKp30, NKp44 and NKp46) on CD3-CD56+NK cell of 13 MM patients and 30 healthy controls were analyzed by flow cytometry. The concentration of soluble NKG2D ligands (MICA, MICB, ULBP1, ULBP2 and ULBP3) in serum was detected by enzyme- linked immunosorbent assay (ELISA), and the cytotoxicity of NK cell against MM cell line by flow cytometry.

Quantifying nanosheet graphene oxide using electrospray-differential mobility analysis.

We report a high-resolution, traceable method to quantify number concentrations and dimensional properties of nanosheet graphene oxide (N-GO) colloids using electrospray-differential mobility analysis (ES-DMA). Transmission electron microscopy (TEM) was employed orthogonally to provide complementary data and imagery of N-GOs. Results show that the equivalent mobility sizes, size distributions, and number concentrations of N-GOs were able to be successfully measured by ES-DMA.

The Prognostic Significance of the Serum p53 Protein Concentration in Chinese Patients with Non-Hodgkin Lymphoma.

Objective: To investigate the prognostic significance of cytogenetic abnormalities, staging, patient factors, and the serum p53 protein concentration in Chinese non-Hodgkin lymphoma (NHL) patients.

Material And Methods: The study included 43 patients with NHL that were identified between August 2003 and December 2008. Patient clinical characteristics patients were determined based on morphological, immunohistochemical, and cytogenetic analysis, and the serum p53 protein concentration was measured quantitatively.

[Study of single nucleotide polymorphism of SOCS gene in typical myeloproliferative neoplasms].

This study was purposed to investigate the effect of mutation and single nucleotide polymorphism (SNP) of suppressor of cytokine signaling (SOCS) on the typical myeloproliferative neoplasms (MPN) and its mechanism. The mutation and SNP of SOCS1, SOCS2, SOCS3 genes in 100 MPN patients were detected by RT-PCR and direct sequencing. The results showed that among 100 cases there were 21 cases with A→C polymorphism in the 63th site nucleotide of the 15 SOCS3 exon (SNP library no reported), 18 cases with A→C polymorphism in the 1779th site nucleotide of the 15 SOCS3 exon, 49 cases with A→G polymorphism in the 2249th site nucleotide of the 15 SOCS3 exon (SNP library no reported), 39 cases with T→C polymorphism in the 2366th site nucleotide of the 15 SOCS3 exon (SNP library no reported), 9 cases with T→C polymorphism in the exon of 15 SOCS2 gene (SNP library no reported).

[Enhanced cytotoxicity against leukemia cells of natural Killer cells from cord blood after expansion in vitro].

Objective: To investigate the enhanced cytotoxicity against leukemia cells of natural Killer (NK) cells from cord blood (CB) after expansion in vitro.

Methods: NK cells was expanded on a layer of trophoblast cells with irradiated K562-mb15-41BBL cell line for 21 days. The levels of receptors on NK cells were detected by flow cytometry.

Human leukocyte antigen (HLA)-DRB1*14 is associated with a high incidence of acute lymphocytic leukemia.

Background: Genetic background and environmental factors play an interactive role in the development of acute lymphocytic leukemia (ALL), and the human leukocyte antigen (HLA) system was noted as an important genetic factor in ALL.

Material And Methods: Due to the high diversity of HLA alleles, our present study assessed the possibility of an association of HLA molecules in ALL patients living in Jiangsu Province, Eastern China. HLAA, -B, and -DRB1 allele distributions in 156 ALL patients (aged 3-54 years) were analyzed and compared with unrelated healthy hematopoietic stem cell donors from the same ethnic and geographic background.

[Clinical and cytogenetic analyses of 45 adult patients with acute lymphoblastic leukemia].

Objective: To analyze the correlation between clinical features and cytogenetic finding of 45 adult patients with acute lymphoblastic leukemia (ALL), and to assess the value of chromosomal examination for the diagnosis and prognosis.

Methods: Fluorescence in situ hybridization (FISH) was utilized for detecting the BCR/ABL fusion gene and P53 gene. Median survival time (MST) of patients was compared using Log-rank test.

P53 deletion is independently associated with increased age and decreased survival in a cohort of Chinese patients with diffuse large B-cell lymphoma.

The purpose of the study was to compare cytogenetic profiles and survivals between elderly and non-elderly Chinese patients with diffuse large B-cell lymphoma (DLBCL). We identified 50 patients with DLBCL and divided them by age into elderly (≥60 years) and non-elderly (< 60 years) groups. We detected deletion of P53 or translocations in Bcl-2, Bcl-6 or c-myc genes by fluorescence in situ hybridization (FISH).

[Prognostic value of t(11; 18) (q21; q21) for gastric mucosa-associated lymphoid tissue lymphoma].

Objective: To investigate the prognostic value of t(11; 18) (q21; q21) in gastric mucosa-associated lymphoid tissue lymphoma.

Methods: A cohort of thirty-six gastric mucosa-associated lymphoid tissue lymphoma patients who were pathologically identify diagnosis from January 1994 to June 2004 were followed up retrospectively and studied using fluorescence in situ hybridization(FISH) technique to detect t(11; 18) (q21; q21) chromosomal translocation on preservative paraffin specimen.

Results: Among thirty-six patients, fifteen (41.

[Abnormal expression of hematopoietic regulatory factors in newly diagnosed patients with acute myeloid leukemia].

The aim of this study was to detect the expressions of transforming growth factor (TGFβ(1)), tumor necrosis factor alpha (TNFα) and leukemia inhibitory factor (LIF) in newly diagnosed patients with acute myeloid leukemia (AML) and investigate the association between serum levels of various cytokines and clinical outcomes. The levels of TGFβ1, TNFα and LIF in patient's plasma were detected by enzyme-linked immunosorbent assays (ELISA) and were compared with healthy controls; bone marrow cell morphology, immunology, cytogenetics examinations (MIC) were performed meanwhile. The results showed that levels of TGFβ1, TNFα and LIF were elevated in AML patients as compared with the controls (13.