Eliminated high lipid inhibition in the anaerobic digestion of food waste for biomethane production by engineered E. coli with cell surface display lipase.

Food waste (FW) with high content of lipid typically inhibits anaerobic digestion (AD) and methane production. In this study, a novel whole-cell catalyst was created to degrade lipid by displaying lipase on the E. coli cells surface to improve FW anaerobic digestion.

Fermentation properties and functional stability of dough starter Jiaozi and Laomian after frozen storage.

Purpose: This study aims to investigate the effects of frozen storage on the stability of traditional dough starters in China.

Methods: The microbial community structure and abundance of related metabolic genes in different fermented sourdough prepared by Jiaozi (JZ) and Laomian (LM) starters before and after frozen storage at -20°C for half a year were analyzed using the shotgun metagenomic sequencing method, and differences in characteristics of texture in steamed bread were also compared by formal methods.

Results: The fermentation ability (FA) and metabolic activities of yeast in the JZH sourdough (started by JZ which was stored at -20°C for half a year) were better than those of LMH sourdough (started by LM which was stored at -20°C for half a year).

Phosphate solubilization and plant growth properties are promoted by a lactic acid bacterium in calcareous soil.

On the basis of good phosphate solubilization ability of a lactic acid bacteria (LAB) strain Limosilactobacillus sp. LF-17, bacterial agent was prepared and applied to calcareous soil to solubilize phosphate and promote the growth of maize seedlings in this study. A pot experiment showed that the plant growth indicators, phosphorus content, and related enzyme activity of the maize rhizospheric soils in the LF treatment (treated with LAB) were the highest compared with those of the JP treatment (treated with phosphate solubilizing bacteria, PSB) and the blank control (CK).

Application of biochar in microbial fuel cells: Characteristic performances, electron-transfer mechanism, and environmental and economic assessments.

Biochar is a by-product of thermochemical conversion of biomass or other carbonaceous materials. Recently, it has garnered extensive attention for its high application potential in microbial fuel cell (MFC) systems owing to its high conductivity and low cost. However, the effects of biochar on MFC system performance have not been comprehensively reviewed, thereby necessitating the evaluation of the efficacy of biochar application in MFCs.

Responses of the microbial community and the production of extracellular polymeric substances to sulfamethazine shocks in a novel two-stage biological contact oxidation system.

Introduction: The biological contact oxidation reactor is an effective technology for the treatment of antibiotic wastewater, but there has been little research investigating its performance on the sulfamethazine wastewater treatment.

Methods: In this study, a novel two-stage biological contact oxidation reactor was used for the first time to explore the impact of sulfamethazine (SMZ) on the performance, microbial community, extracellular polymeric substances (EPS), and antibiotic-resistant genes (ARGs).

Results: The chemical oxygen demand (COD) and ammonia nitrogen (-N) removal efficiencies kept stable at 86.

Purification and characterization of an alkali-organic solvent-stable laccase with dye decolorization capacity from newly isolated Lysinibacillus fusiformis W11.

A new Lysinibacillus fusiformis strain with abundant laccase activity was isolated from soil under forest rotted leaf and identified as L. fusiformis W11 based on its 16S rRNA gene sequence and physiological characteristics. The laccase LfuLac was purified and characterized.

sp. nov., a bacterium isolated from lake sediment.

A taxonomic identification using polyphasic approach was performed on strain TH16-21, which was isolated from the interfacial sediment of Taihu Lake, PR China. Strain TH16-21 was Gram-stain-negative, aerobic, rod-shaped and catalase-positive. Phylogenetic analysis based on the 16S rRNA gene and genomic sequences indicated that strain TH16-21 was classified within the genus of .

Characterization of a Novel One-Domain Halotolerant Laccase from Parageobacillus thermoglucosidasius and Its Application in Dye Decolorization.

Laccases are widespread multi-copper oxidases and generally classified into three-domain laccases and two-domain laccases. In this study, a novel laccase PthLac from Parageobacillus thermoglucosidasius harbored only one domain of Cu-oxidase_4 and showed no sequence relatedness or structure similarity to three-domain and two-domain laccases. PthLac was heterologously expressed in Escherichia coli, purified, and characterized.

Responses of microbial community and antibiotic resistance genes to co-existence of chloramphenicol and salinity.

In recent years, the risk from environmental pollution caused by chloramphenicol (CAP) has emerged as a serious concern worldwide, especially for the co-selection of antibiotic resistance microorganisms simultaneously exposed to CAP and salts. In this study, the multistage contact oxidation reactor (MCOR) was employed for the first time to treat the CAP wastewater under the co-existence of CAP (10-80 mg/L) and salinity (0-30 g/L NaCl). The CAP removal efficiency reached 91.

sp. nov., a bacterium isolated from lake sediment.

A novel bacterium, designated NAS39, was isolated from the interfacial sediment of Taihu Lake in PR China and its taxonomic position was investigated by using a polyphasic approach. Cells of the isolate were Gram-stain-negative, aerobic, non-motile, catalase-positive, yellow and rod-shaped. Phylogenetic analyses based on 16S rRNA gene sequences supported that strain NAS39 formed a cluster within the genus , and was most closely related to LB2P30 (98.

Comparison of the effects of acetic acid bacteria and lactic acid bacteria on the microbial diversity of and the functional pathways in dough as revealed by high-throughput metagenomics sequencing.

Knowledge of the effects of various strains of acetic acid bacteria (AAB) on sourdough remains limited. In this study, the diversity of microbial taxa in sourdoughs fermented by different starters was assessed and their functional capacity was evaluated via high-throughput metagenomics sequencing. Results showed that Erwinia (29.

sp. nov., a red-pigmented bacterium from freshwater lake sediment, and proposal of as a latter heterotypic synonym of .

A taxonomic identification using a polyphasic approach was performed on strain NBS58-1, which was isolated from the interfacial sediment of Taihu Lake in China. Strain NBS58-1 was Gram-stain-negative, aerobic, non-spore-forming and catalase-positive. Phylogenetic analyses based on 16S rRNA gene and three housekeeping genes (, and ) sequences supported the position that strain NBS58-1 should be classified within the genus .

sp. nov., an actinobacterium isolated from lake sediment.

A novel strain, designated NS18, was isolated from sediment sampled at Taihu Lake, PR China. Cells of the isolate were spherical, aerobic, non-motile, Gram-stain-positive and non-endospore-forming. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NS18 clustered in a clade of the genus .

Biodegradation of phenolic compounds in high saline wastewater by biofilms adhering on aerated membranes.

High saline phenolic wastewater is a typical toxic and refractory industrial wastewater. A single membrane-aerated biofilm reactor (MABR) was used to treat wastewater containing phenol, p-nitrophenol and hydroquinone under increasing phenolic loading and salinity conditions. More than 95 % of phenolic compounds were removed, and a removal efficiency of 8.

Degradation of acetochlor and beneficial effect of phosphate-solubilizing sp. ACD-9 on maize seedlings.

The biodegradation of acetochlor in solution and soil and improvements in the growth of maize seedlings by a phosphate-solubilizing bacterial strain were investigated in this research. The strain sp. ACD-9 optimally degraded acetochlor at pH 6.

sp. nov., a novel species originating from lake sediment.

An aerobic, Gram-stain-negative, non-spore-forming and rod-shaped bacterial strain, designated N8, was isolated from the interfacial sediment of Taihu Lake in PR China. The strain formed white to blue colonies on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N8 represented a member of the genus and was most closely related to A1-9 (97.

sp. nov., isolated from lake sediment.

A novel bacterial strain, designated NS28, was isolated from interfacial sediment sampled at Taihu Lake, PR China. Cells were rod-shaped, Gram-negative, aerobic and non-motile on Reasoner's 2A medium. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NS28 was most closely related to species from the genus , with 98.

Antioxidant activity of a polysaccharide produced by Chaetomium globosum CGMCC 6882.

In present work, a polysaccharide (GCP-WS) was successfully produced by Chaetomium globosum CGMCC 6882 from wheat straw. Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.

Treatment of real sodium saccharin wastewater using multistage contact oxidation reactor and microbial community analysis.

In this study, multistage contact oxidation reactor (MCOR) with a novel carrier was used for treatment of high-strength sodium saccharin wastewater (SSW) under stepwise increasing salinities from 1.0% to 8.0%.

Description of sp. nov., a halophilic bacterium isolated from lake sediment, and emended description of the genus .

A Gram-stain-positive, strictly aerobic, motile and rod-shaped bacterium, designated strain LJ137, was isolated from the sediment of Taihu Lake in China. A polyphasic approach was used to investigate its taxonomic position. Strain LJ137 grew optimally at pH 7.

Brevundimonas lutea sp. nov., isolated from lake sediment.

A novel Gram-negative, aerobic, rod-shaped bacterium, designated NS26, was isolated from a sediment sample collected from Taihu Lake in China. Colonies were orange, circular, smooth and neat-edged on Reasoner's 2A agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NS26 belonged to the genus Brevundimonas and had the closest relationship with Brevundimonas halotolerans DSM 24448 (96.

Altererythrobacter amylolyticus sp. nov., isolated from lake sediment.

An aerobic, motile, Gram-stain-negative bacterium, designated strain NS1, was isolated from interfacial sediment from Taihu Lake, China. The strain formed yellow colonies on R2A medium. Cells were ovoid to rod-shaped and non-spore-forming.

Rufibacter sediminis sp. nov., isolated from freshwater lake sediment.

A novel Gram-stain-negative, aerobic and rod-shaped bacterial strain, designated H-1, was isolated from the interfacial sediment of Taihu Lake in China and characterized by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Rufibacter immobilis MCC P1 and Rufibacter tibetensis 1351, with sequence similarities of 98.0 and 97.