Publications by authors named "Jianhai Dong"
Gene identification and expression analysis of 86,136 Expressed Sequence Tags (EST) from the rice genome.
Genomics Proteomics Bioinformatics
February 2003
Expressed Sequence Tag (EST) analysis has pioneered genome-wide gene discovery and expression profiling. In order to establish a gene expression index in the rice cultivar indica, we sequenced and analyzed 86,136 ESTs from nine rice cDNA libraries from the super hybrid cultivar LYP9 and its parental cultivars. We assembled these ESTs into 13,232 contigs and leave 8,976 singletons.
View Article and Find Full Text PDFAlternative splicing is an important cellular mechanism that increases the diversity of gene products. The number of alternatively spliced genes reported so far in plants is much smaller than that in mammals, but is increasing as a result of the explosive growth of available EST and genomic sequences. We have searched for all alternatively spliced genes reported in GenBank and PubMed in all plant species under Viridiplantae.
View Article and Find Full Text PDFArticle Synopsis
- A draft sequence of the genome for the rice subspecies Oryza sativa L. ssp. indica has been created using whole-genome shotgun sequencing, revealing a size of 466 megabases and estimating between 46,022 and 55,615 genes.
- About 92% of the assembled genome has functional coverage, with a significant portion (42.2%) consisting of exact 20-nucleotide repeats and most transposons found in intergenic regions.
- While around 80.6% of genes in Arabidopsis thaliana (another plant) have homologs in rice, only 49.4% of rice genes have identifiable homologs in A. thaliana, which may be attributed to variations in