Abnormal lipid metabolism plays an important role in cancer development. In this study, nontargeted lipidomic study on 230 tissue specimens from 79 nonsmall cell lung cancer (NSCLC) patients was conducted using ultraperformance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS). Downregulation of sphingosine and medium-long-chain ceramides and short-medium-chain acylcarnitine, upregulation of long-chain acylcarnitine C20:0, and enhanced histamine methylation were revealed in NSCLC tissues.
View Article and Find Full Text PDFA tumor-targeting fluorescent probe has attracted increasing interest in fluorescent imaging for the noninvasive detection of cancers in recent years. Sulfonamide-containing naphthalimide derivatives (SN-2NI, SD-NI) were synthesized by the incorporation of N-butyl-4-ethyldiamino-1,8-naphthalene imide (NI) into sulfonamide (SN) and sulfadiazine (SD) as the tumor-targeting groups, respectively. These derivatives were further characterized by mass spectrometry (MS), nuclear magnetic resonance spectroscopy (H NMR), Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV), and a fluorescence assay.
View Article and Find Full Text PDFBackground: To evaluate postoperative clinical outcomes and analyze influencing factors for patients with thymic epithelial tumors over 3 years after operation.
Methods: Patients with thymic epithelial tumors (TETs) who underwent surgical treatment in the Department of Thoracic Surgery at Beijing Hospital from January 2011 to May 2019 were retrospectively enrolled in the study. Basic patient information, clinical, pathological, and perioperative data were collected.
Background: To evaluate the surgical safety in myasthenia gravis (MG) patients aged 65 and over.
Methods: A total of 564 patients with MG who underwent surgery in the Department of Thoracic Surgery of Beijing Hospital from November 2011 to March 2022 were included in the study and divided into two groups taking the age of 65 as the boundary. Perioperative data of patients were recorded and statistically analyzed.
Objective: To study the influencing factors of myasthenic crisis in non-thymoma myasthenia gravis (MG) patients during perioperative period.
Methods: We retrospectively analyzed a total of 387 non-thymoma MG patients who underwent extended thymoma resection in the Department of Thoracic Surgery of Beijing Hospital from February 2011 to December 2021, recorded ASA score, Osserman classification, preoperative course, pyridostigmine dosage, operation method, operation time, and intraoperative blood loss, then analyzed the factors associated with postoperative myasthenic crisis by univariate and multivariate logistic regression.
Results: Osserman classification IIB + III + IV (P < 0.
Objective: To study the influencing factors of myasthenic crisis in patients with myasthenia gravis during perioperative period.
Methods: A total of 564 myasthenia gravis (MG) patients who underwent standard expanded resection of thymoma/thymoma in the Department of Thoracic Surgery of Beijing Hospital from January 2011 to March 2022 were retrospectively included in the study. Clinical indicators such as gender, age, thymoma, American Society of Anesthesiologists (ASA) score, operation time, intraoperative blood loss, and some others were recorded.
Environ Sci Pollut Res Int
January 2023
The use of cement and fly ash (FA) to prepare cemented tailings backfill (CTB) lowers backfill mining costs while also reducing pollution caused by the accumulation of waste materials like tailings and FA, making it a green backfill mining process. While adding FA to CTB may reduce costs, too much FA might weaken CTB's strength property. Mechanical tests were used to explore the effects of FA content and curing time on the uniaxial compressive strength (UCS) and deformation modulus of CTB in this research.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
August 2022
Cemented tailings backfill (CTB) is widely adopted to ensure the safety of underground goafs and mitigate environmental risks. Fly ash (FA) and calcium formate (CF) are common industrial by-products that improve the mechanical performance of CTB. How the coupling of the two components affects the strength development is not yet well-understood.
View Article and Find Full Text PDFAlthough two-dimensional (2D) cell cultures are the standard in cell research, one pivotal disadvantage is the lack of cell-cell and cell-extracellular matrix (ECM) signaling in the culture milieu. However, such signals occur in three-dimensional (3D) in vivo environments and are essential for cell differentiation, proliferation, and a range of cellular functions. In this study, we developed a microfluidic device to proliferate and differentiate functional adipose tissue and adipocytes by utilizing 3D cell culture technology.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
April 2022
Cemented tailings backfill (CTB) is the most economical and environmental method to recycle tailings and fly ash (FA) for filling mining, but the high content of FA will weaken its strength property. This paper aims to use calcium formate (CF) as an activator to stimulate the activity of FA, thereby enhancing the mechanical property of CTB. The influence of FA and CF content on the stress-strain behavior, dilatancy deformation, and compressive strength of CTB was investigated using uniaxial compression test and scanning electron microscope.
View Article and Find Full Text PDFRecycling mining wastes to produce cemented tailings backfill (CTB) is the optimal approach to eliminate the environmental pollution caused by their accumulation. However, its low strength limits its application. Using calcium formate (CF) as an accelerator for improving its mechanical properties is of great significance to promote sustainable development.
View Article and Find Full Text PDFSome Bacillus thuringiensis (Bt) strains produce dipteran-active toxins and can control larval mosquitoes. We identified a novel mosquitocidal toxin named Xpp81Aa1 with the thioredoxin domain from Bt strain HSY204. This toxin has very little sequence similarity to the three-domain Cry toxin and Cyt toxins and has significant toxicity to Aedes aegypti larvae.
View Article and Find Full Text PDFFilling mining with cemented waste rock backfill (CWRB) is an optimal approach to eliminate the gangue waste pollution. To efficiently evaluate the gangue recycling and its advantage in structure protection, the effects of the confining pressure, cement dosage, and aggregate particle size distribution (PSD) on the creep behavior of CWRB were investigated. Burgers creep model was used to characterize the visco-elastic characteristics of CWRB, a visco-elastic-plastic creep model was established to describe its creep behavior on this basis.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
April 2021
The most economical, environmental, and friendly method for recycling gangue is filling mining with cemented waste rock backfill (CWRB), which solves the environmental problems caused by gangue discharge and reduces the mining damages. Evaluating the mesoscopic structure of CWRB is of great significance for maximizing the utilization of gangue recycling and improving the economic benefits of filling mining. This paper constructed the particle flow model of cemented waste rock backfill (CWRB) considering particle size distribution (PSD) of aggregates and hydration of cementing material to investigate the effect of the PSD of aggregates on its mesoscopic structural evolution.
View Article and Find Full Text PDFProteases play an essential role in the four sequential but overlapping phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. In chronic wounds, excessive protease secretion damages the newly formed extracellular matrix, thereby delaying or preventing the normal healing process. Peptide-based fluorogenic sensors provide a visual platform to sense and analyze protease activity through changes in the fluorescence intensity.
View Article and Find Full Text PDFIntelligent hydrogels responsive to external stimuli have been widely studied due to their great potentials for applications in artificial muscles, soft robotics, sensors and actuators. However, the weak mechanical properties, narrow response range, and slow response speed of many responsive hydrogels have hindered practical applications. In this paper, tough multi-responsive hydrogels were synthesized by using vinyl-functionalized triblock copolymer micelles as macro-crosslinkers and N-isopropyl acrylamide (NIPAM) and acrylamide (AAm) or 2-(dimethylamino)ethyl methacrylate (DMAEMA) and 2-acrylamido-2-methyl-1-propane-sulfonic acid (AMPS) as monomers.
View Article and Find Full Text PDFBacillus thuringensis (Bt) vegetative insecticidal proteins share no sequence or structural homology with the known Cry proteins. The activity of Vip3Aa is generally considered to be restricted to lepidopteran insects. In this paper, the vip3Aa gene was cloned from Bt subsp.
View Article and Find Full Text PDFFunctionalized alginate microbeads (MB) have been widely used for three-dimensional (3D) culture of cells and creating biomimetic tissue models. However, conventional methods for preparing these MB suffer from poor polydispersity, due to coalescence of droplets during the gelation process and post-aggregation. It remains an immense challenge to prepare alginate MB with narrow size distribution and uniform shape, especially when their diameters are similar to the size of cells.
View Article and Find Full Text PDFInvestigating the effect of the aggregate gradation on the material properties of cemented rockfill is significant for the green mining, economic benefit and engineering safety. Consequently, the ultrasonic test, uniaxial compression experiment and acoustic emission (AE) monitor on cemented rockfill were carried out, for which the aggregate satisfied Talbot gradation. The dilatancy behavior and AE characteristic of cemented rockfill under load were investigated.
View Article and Find Full Text PDFIt is important to study the mechanical properties of cracked rock to understand the engineering behavior of cracked rock mass. Consequently, the influence of the length of pre-existing fissures on the strength, deformation, acoustic emission (AE) and failure characteristics of cracked rock specimen was analyzed, and the optimal selection of strength parameter in engineering design was discussed. The results show that the strength parameters (stress of dilatancy onset and uniaxial compressive strength) and deformation parameters (axial strain and circumferential strain at dilatancy onset and peak point) of cracked rock specimen decrease with the increase of the number of pre-existing fissures, and the relations which can use the negative exponential function to fit.
View Article and Find Full Text PDFCorrection for 'Multi-responsive nanocomposite hydrogels with high strength and toughness' by Jingli Yang et al., J. Mater.
View Article and Find Full Text PDFMulti-responsive hydrogels with high strength have great significance for potential applications in smart soft devices. However, it remains a challenge to incorporate multiple responsive moieties with energy dissipation mechanisms. Herein, multi-responsive nanocomposite hydrogels with high compressive strength and toughness were synthesized via in situ copolymerization of N-isopropylacrylamide (NIPAM) and acryloyloxyethyltrimethyl ammonium chloride (DAC) in an aqueous dispersion of exfoliated LAPONITE® RDS with a minute amount of N,N'-methylenebisacrylamide (MBAA) as a crosslinker.
View Article and Find Full Text PDFScientificWorldJournal
July 2014
RNA interference (RNAi) was first literaturally reported in 1998 and has become rapidly a promising tool for therapeutic applications in gene therapy. In a typical RNAi process, small interfering RNAs (siRNA) are used to specifically downregulate the expression of the targeted gene, known as the term "gene silencing." One key point for successful gene silencing is to employ a safe and efficient siRNA delivery system.
View Article and Find Full Text PDFThe bio-responsive reversible assembly of silica nanoparticles grafted with complementary DNA strands containing an ATP-sensitive aptamer sequence was studied. The optimal conditions for ATP-induced specific disassembly of the bionanocomposite network were identified, highlighting the existing competition between ATP/aptamer interactions and DNA duplex stability. A new, dialysis-based method for ATP removal was proposed, allowing partial re-formation of the initial DNA duplexes.
View Article and Find Full Text PDFA novel supramolecular framework, catena-poly[[[aqua(2-phenylquinoline-4-carboxylato-κO)silver(I)]-μ-4,4'-bipyridine-κ(2)N:N'] dihydrate], {[Ag(C16H10NO2)(C10H8N2)(H2O)]·2H2O}n, has been synthesized and structurally characterized. The Ag(I) centres are four-coordinated and bridged by 4,4'-bipyridine (4,4'-bipy) ligands to form a one-dimensional Ag-bipy chain. The Ag-bipy chains are further linked together by intermolecular O-H···O and O-H···N hydrogen-bonding interactions between adjacent chains, resulting in a three-dimensional framework.
View Article and Find Full Text PDF