CRISPR-Enhanced Photocurrent Polarity Switching for Dual-lncRNA Detection Combining Deep Learning for Cancer Diagnosis.

Abnormal expression in long noncoding RNAs (lncRNAs) is closely associated with cancers. Herein, a novel CRISPR/Cas13a-enhanced photocurrent-polarity-switching photoelectrochemical (PEC) biosensor was engineered for the joint detection of dual lncRNAs, using deep learning (DL) to assist in cancer diagnosis. After target lncRNA-activated CRISPR/Cas13a cleaves to induce DNAzyme bidirectional walkers with the help of cofactor Mg, nitrogen-doped carbon-Cu/CuO octahedra are introduced into the biosensor, producing a photocurrent in the opposite direction of CdS quantum dots (QDs).

A novel dual-model photoelectrochemical/electrochemical sensor based on Z-scheme TiO disks/methylene blue for kanamycin detection.

Herein, a new dual-model photoelectrochemical (PEC)/electrochemical (EC) sensor based on Z-scheme titanium dioxide (TiO) disk/methylene blue (MB) sensibilization for the detection of kanamycin (Kana) was developed. Metal-organic framework-derived porous TiO disks were synthesized and exhibited excellent anodic photocurrent under visible light excitation. Subsequently, amino-labeled double-stranded DNA (dsDNA) was introduced into the modified electrode.

A signal-switchable photoelectrochemical biosensor for ultrasensitive detection of long non-coding RNA in cancer cells.

Long non-coding RNA (LncRNA) as an emerging tumor biomarker plays a key factor in the early diagnosis of cancer. Herein, an innovative signal-switchable photoelectrochemical (PEC) biosensor based on ZrO@CuO bimetallic oxides and T7 Exo-assisted signal amplification is reported for the ultrasensitive and selective detection of lncRNA (HOX gene antisense intergenic RNA, HOTAIR) in cancer cells. Firstly, MOFs-derived TiO nanodisks as an excellent photoactive material show an anodic background signal.

MOFs-derived CoO@MnO@Carbon dots with enhanced nanozymes activity for photoelectrochemical detection of cancer cells in whole blood.

Nanozymes have attracted widespread attention, and rationally designing high-activity nanozymes to improve their application performance are a long-term objective. Herein, taking metal-organic frameworks-derived CoO polyhedron with large surface area and high porosity as nanoconfinement carriers, CoO@MnO@CDs polyhedron was successfully synthesized by the room-temperature reduction of MnO ions and physical load of carbon dots (CDs). Through cancer cells-triggered double antibody sandwich strategy, the CoO@MnO@CDs polyhedron were introduced to the TiO nanoparticle (NPs) modified electrode, leading to the decreased photocurrent.

A dual-model immobilization-free photoelectrochemical/visual colorimetric bioanalysis based on microemulsion self-assemblies mediated multifunctional signal amplification strategy.

Herein, a novel silver ion-loaded gold microemulsion assemblies (Au/Ag MAs) mediated multifunctional signal amplification strategy was proposed to construct a sensitive immobilization-free photoelectrochemical (PEC)/colorimetric biosensor for carcinoembryonic antigen (CEA) detection. Through the sandwiched reaction among CEA, the CEA aptamer (DNA1) loaded on the Au nanoparticles (NPs) functionalized iron oxide (FeO) nanospheres and another CEA aptamer (DNA2) immobilized on Au/Ag MAs, a complex is formed and acquired by magnetic separation. Then, Au/Ag MAs of the complex are disassembled into Au NPs and Ag ions driven by an acetone response, and the obtained demulsification solution is transferred to the cadmium sulfide/cadmium telluride (CdS/CdTe) photoactive composites modified electrode.

[Prokaryotic expression and polyclonal antibodies preparation and identification of human focal adhesion kinase (FAK)].

Objective To Clone, express, and purify the focal adhesion kinase (FAK) gene C-terminal focal adhesion location sequence (aa798-aa1041), and to prepare and identify the rabbit anti-FAK polyclonal antibodies. Methods The C-terminal (2671 bp-3402 bp) gene of the FAK gene was amplified by PCR in vitro and cloned into pCZN1 vector to construct a pCZN1-FAK recombinant expression vector. The recombinant expression vector was transformed into E.