Mental health status of infected children between 7 to 12 years old in Fangcang Shelter Hospital during the COVID-19 Shanghai lockdown in 2022: a cross-section study.

Background: There has been an increase in research on the potential adverse effects on children's mental health, especially depression and anxiety, during the coronavirus disease 2019 (COVID-19) pandemic over the past few months. Therefore, the aim of the present study was to investigate depression and anxiety symptoms among children in shelter hospitals during the 2022 Shanghai lockdown.

Methods: A total of 98 infected children aged 7-12 years were enrolled in this study between April 19 and June 4, 2022.

Application of the Cycle Management Model in Improving Outpatient Appointment Services.

To explore the application of plan-do-check-action (PDCA) cycle management model in the management outpatient appointment, and improve the efficiency of outpatient appointment services. The data of outpatients from January 2019 to December 2020 were collected from a tertiary class B general hospital affiliated to a university in Shanghai. Through the investigation and analysis of the current situation, the reasons were found for the low rate of outpatient appointment.

Coronavirus Disease 2019 (COVID-19) Learning Online: A Flipped Classroom Based on Micro-Learning Combined with Case-Based Learning in Undergraduate Medical Students.

Background: During the coronavirus disease 2019 (COVID-19) pandemic, medical colleges in China had to use online teaching. This study explored the effect of COVID-19 knowledge learning online in a flipped classroom based on micro-learning combined with case-based learning (CBL).

Methods: There were 74 undergraduate medical students who were randomly grouped to an observation group and a control group with 37 participants in each virtual classroom on the Network Teaching Platform.

Pelvic floor muscle exercise for chronic low back pain.

Objective: To assess the effect of pelvic floor muscle exercise in patients with chronic low back pain.

Methods: Adults (aged ≥ 18 years) with chronic low back pain (with or without radiculopathy) were randomized to undergo either routine treatment (ultrasonography, short wave diathermy and lumbar strengthening exercises; control group) or routine treatment with pelvic floor exercises (intervention group) for 24 weeks. Pain, disability (Oswestry Disability Index [ODI] score) and trunk muscle function were assessed at baseline and after completion of treatment.

[Effects of controlling specific dangerous pesticides on prevention of acute pesticide poisoning in rural area].

Objective: To investigate the effects of controlling the specific dangerous pesticides on prevention of acute pesticide poisoning in rural area.

Methods: The data of reported cases of pesticide poisoning were analyzed to find out the specific dangerous pesticide in acute pesticide poisoning. Then the occurrence of occupational pesticide poisoning and fatality of non-occupational pesticide poisoning were estimated under the hypothesis of removing the specific dangerous pesticides.

Influence of vitamin D receptor haplotypes on blood lead concentrations in environmentally exposed children of Uygur and Han populations.

Objective: To explore the association between polymorphism of vitamin D receptor (VDR) and lead poisoning in Uygur and Han children in China.

Methods: The BsmI, ApaI and TaqI restriction sites of VDR genotyping were determined by polymerase chain reaction-restriction fragment length polymorphism in 443 Uygur and 469 Han children from Xinjiang province. The correlation between the polymorphism of VDR haplotypes and blood lead levels was explored.

[Polymorphism of vitamin D receptor and lead poisoning genetic polymorphism in Han, Uygur and Kazak child in China].

Objective: To explore the relationship between the polymorphism of vitamin D receptor (VDR) and genetic polymorphism in Han, uygur and Kazak child in China.

Methods: The VDR genotyping was determined by PCR-RFLP in 469 Han, 443 Uygur and 516 Kazak child from Xinjiang province. The correlation between the polymorphism of VDR and blood lead levels were explored.

[Detection of Y chromosome microdeletions in semen of patients with azoospermia: study of 241 cases].

Objective: To set up a simple and reliable method to screen Y chromosome microdeletions in semen of azoospermic patients, and to explore the incidence and loci of Y chromosome microdeletions in Chinese azoospermia.

Methods: Two hundred and forty-one semen samples, 51 containing blood, were collected from 241 Chinese azoospermic patients. 45 normal semen samples and 1 anticoagulated blood sample from female were used as controls.

Lack of association of delta-aminolevulinic acid dehydratase genotype with blood lead levels in environmentally exposed children of Uygur and Han populations.

Aim: A cross-section study was conducted to explore the association between polymorphism of delta-aminolevulinic acid dehydratase (ALAD) and lead poisoning in Uygur and Han children in China.

Methods: The ALAD genotyping was determined by PCR-RFLP in 443 Uygur and 469 Han children aged 6-10 years from Urumqi in Xinjiang province.

Results: The blood lead levels of 912 environmentally exposed children ranged from 0.

[Y chromosome microdeletions of 664 Chinese men with azoospermia or severe oligozoospermia].

Objective: To explore the incidence and location of Y chromosome microdeletions in Chinese azoospermia and severe oligozoospermia, as well as the relationship between the deletion region and testicular phenotype.

Methods: Semen samples or blood samples were collected from 664 Chinese patients (584 with azoospermia and 80 with severe oligozoospermia). DNA was extracted by incubating cells with a lysis buffer containing polymerase chain reaction (PCR) buffer and proteinase K, and was assayed for deletion of 15 sequence tagged sites (including 6 loci recommended by European Academy of Andrology and European Molecular Genetics Quality Network (EAA/EMQN) distributed in AZFa, AZFb and AZFc by 4 multiplex PCRs.