Performance verification and clinical evaluation of the NAP-Fluo Cycler system for detecting five genital tract pathogens based on microfluidic technology.

Introduction: Sexually transmitted infections (STIs) are among the most common infectious diseases worldwide, often leading to coinfections. Timely detection of genital tract pathogens in at-risk populations is crucial for preventing STIs. We evaluated the NAP-Fluo Cycler System, an innovative microfluidic nucleic acid detection platform, for its ability to simultaneously identify (CT), (NG), (UU), (MG), and (MH) in urethral or cervical secretions.

Functional testing is a complementary tool for the diagnosis of vaginitis.

Objective: Vaginal microbiota evaluation is a methodology widely used in China to diagnose various vaginal inflammatory diseases. Although vaginal microbiota evaluation has many advantages, it is time-consuming and requires highly skilled and experienced operators. Here, we investigated a six-index functional test that analyzed pH, hydrogen peroxide (HO), leukocyte esterase (LEU), sialidase (SNA), β-glucuronidase (GUS), and acetylglucossidase (NAG), and determined its diagnostic value by comparing it with morphological tests of vaginal microbiota.

Diagnostic performance of double inversion recovery MRI sequence for synovitis of the wrist joints in rheumatoid arthritis.

Purpose: To assess the diagnostic accuracy of double inversion recovery (DIR) magnetic resonance imaging (MRI) sequences for synovitis of the wrist joints in patients with rheumatoid arthritis (RA).

Material And Methods: Participants with newly diagnosed RA were enrolled between November 2019 and November 2020. MRI examinations of the wrist joints were performed using a contrast-enhanced T1-weighted imaging sequence (CE-T1WI) and DIR sequence.

Seismic Performance of Precast Short-Limb Shear Wall with the Bundled Connection at Different Axial Compression Ratios.

In order to investigate the seismic performance of a precast shear wall with a new bundled connection under a high axial compressive ratio, three full-scale precast short-limb shear walls and one full-scale cast-in-place short-limb shear wall were manufactured and loaded under cycling loading. The results show that the precast short-limb shear wall with a new bundled connection has a similar damage mode and crack evolution to the cast-in-place shear wall. Under the same axial compression ratio, the bearing capacity, ductility coefficient, stiffness, and energy dissipation capacity of the precast short-limb shear wall were better, and its seismic performance is related to the axial compression ratio, with the increase of the axial compression ratio.

An Approximately Isotropic Origami Honeycomb Structure and Its Energy Absorption Behaviors.

Honeycomb structures have a wide range of applications owing to their light weight and promising energy absorption features. However, a conventional honeycomb structure is designed to absorb impact energy only in the out-of-plane direction and demonstrates unsatisfactory performance when the impact energy originates from a different direction. In this study, we proposed an origami honeycomb structure with the aim of providing an approximately isotropic energy absorption performance.

Hypothermia impairs glymphatic drainage in traumatic brain injury as assessed by dynamic contrast-enhanced MRI with intrathecal contrast.

Introduction: The impact of hypothermia on the impaired drainage function of the glymphatic system in traumatic brain injury (TBI) is not understood.

Methods: Male Sprague-Dawley rats undergoing controlled cortical impact injury (CCI) were subjected to hypothermia or normothermia treatment. The rats undergoing sham surgery without CCI were used as the control.

Ratchetlike motion of helical bilayers induced by boundary constraints.

We show that application of boundary constraints generates unusual folding behaviors in responsive (swellable) helical bilayer strips. Unlike the smooth folding trajectories typical of free helical bilayers, the boundary-constrained bilayers exhibit intermittent folding behaviors characterized by rapid, steplike movements. We experimentally study bilayer strips as they swell and fold, and we propose a simple model to explain the emergence of ratchetlike behavior.

Buckling suppression of a thin-walled Miura-origami patterned tube.

In order to improve energy absorption capacity of tubes under axial compression, this work introduces an octagon tube patterned by curved Miura origami pattern and aims at suppressing global buckling of the tube via an appropriate fold line scheme. By categorizing the fold lines of the tube into two types (hinge-like lines and continuous lines) and allocating them to different positions, four arrangement schemes of the lines are developed. Through numerical comparison in force-displacement curve, stress distribution and lateral deformation capacity among four 3-level patterned tubes under different schemes, Scheme 4 (inclined valley lines as hinge-like lines and the others as continuous lines) is found to outperform the others in suppressing global buckling by reducing the magnitude of lateral deformation by up to 59.

Wearable Triboelectric Sensors Enabled Gait Analysis and Waist Motion Capture for IoT-Based Smart Healthcare Applications.

Gait and waist motions always contain massive personnel information and it is feasible to extract these data via wearable electronics for identification and healthcare based on the Internet of Things (IoT). There also remains a demand to develop a cost-effective human-machine interface to enhance the immersion during the long-term rehabilitation. Meanwhile, triboelectric nanogenerator (TENG) revealing its merits in both wearable electronics and IoT tends to be a possible solution.

Enhanced Defluoridation Using Novel Millisphere Nanocomposite of La-Doped Li-Al Layered Double Hydroxides Supported by Polymeric Anion Exchanger.

A novel nanocomposite bead LaLiAl-LDH@201 was fabricated by doping a small amount of La into nanocrystalline Li/Al layered double hydroxides (LDHs) pre-confined inside polystyrene anion exchanger D201 (LiAl-LDH@201). A systematic characterisation of the resultant LaLiAl-LDH@201 (XRD, SEM-EDS, TEM-EDS, and XPS) evidenced the successful incorporation of La into the Li/Al LDHs, with their interlayer distance expanded to allow more exchangeable sites for fluoride uptake. The resultant LaLiAl-LDH@201 showed high and stable defluoridation performance over a wide range of pH from 4 to 9.

Design, synthesis and evaluate of novel dual FGFR1 and HDAC inhibitors bearing an indazole scaffold.

Both histone deacetylase (HDAC) and fibroblast growth factor receptor (FGFR) are important targets for cancer therapy. Although combining dual HDAC pharmacophore with tyrosine kinase inhibitors (TKIs) had achieved a successful progress, dual HDAC/FGFR1 inhibitors haven't been reported yet. Herein, we designed a series of hybrids bearing 1H-indazol-3-amine and benzohydroxamic acids scaffold with scaffold hopping and molecular hybridization strategies.

Enhanced fluoride removal by La-doped Li/Al layered double hydroxides.

In this study La intercalated Li/Al layered double hydroxide (LDH) was developed for efficient water defluoridation. The La-modified material, i.e.

Efficient defluoridation of water using reusable nanocrystalline layered double hydroxides impregnated polystyrene anion exchanger.

Water decontamination from fluoride is still a challenging task of global concern. Recently, Al-based layered double hydroxides (LDHs) have been extensively studied for specific fluoride adsorption from water. Unfortunately, they cannot be readily applied in scaled-up application due to their ultrafine particles as well as the regeneration issues caused by their poor stability at alkaline pHs.

Arsenate Adsorption by Hydrous Ferric Oxide Nanoparticles Embedded in Cross-linked Anion Exchanger: Effect of the Host Pore Structure.

Three composite adsorbents were fabricated via confined growth of hydrous ferric oxide (HFO) nanoparticles within cross-linked anion exchangers (NS) of different pore size distributions to investigate the effect of host pore structure on the adsorption of As(V). With the decrease in the average pore size of the NS hosts from 38.7 to 9.

Minor dammarane saponins from the hongshen extract of Shenmai injection.

A new dammarane-type triterpenoid saponin, (20R)-ginsenoside ST(2) (1), along with five known saponins was isolated from the hongshen extract of Shenmai injection. The structure of 1 was elucidated to be (20R)-dammar-23(E)-ene-3β,6α,12β,20,25-pentol 6-O-β-D-glucopyranoside by means of spectroscopic methods.

Tumor antigen delivered by Salmonella III secretion protein fused with heat shock protein 70 induces protection and eradication against murine melanoma.

Attenuated Salmonella typhimurium possess the ability to stimulate innate immune responses and preferentially allocate within the solid tumor. These two main characteristics make attenuated Salmonella one of the most attractive vehicles for development of vaccine and also targeted cancer therapies. However, location of Salmonella prevents the process of antigen presentation.

The treatment and prevention of mouse melanoma with an oral DNA vaccine carried by attenuated Salmonella typhimurium.

Therapeutic vaccines of cancer are attractive for their capacity of breaking the immune tolerance and invoking long-term immune response targeting cancer cells without autoimmunity. An efficient antigen delivery system is the key issue of developing an effective cancer vaccine. Attenuated Salmonella typhimurium as the carrier of cancer vaccine are able to transfer DNA from the prokaryote to the eukaryote and preferentially replicate within the tumor tissue.

Study of imidaclothiz residues in cabbage and soil by HPLC with UV detection.

The HPLC method for determination of imidaclothiz residue in cabbage and soil was developed, and its degradation and final residue were studied. The mean accuracies of the analytical method were 92.0-93.

The intrabody targeting of hTERT attenuates the immortality of cancer cells.

hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells.

Adsorption of phenolic compounds from aqueous solutions by aminated hypercrosslinked polymers.

Two novel polymers (NJ-1 and NJ-2) were synthesized by chemically modified a hypercrosslinked polymer NJ-0 with dimethylamine and trimethylamine, respectively. The comparison of the adsorption properties of the three polymers toward phenol, resorcin and phloroglucin was made. The study focused on the static equilibrium adsorption behaviors and the adsorption thermodynamics.

Equilibrium and kinetic studies on the adsorption of aniline compounds from aqueous phase onto bifunctional polymeric adsorbent with sulfonic groups.

In the present study, a hydrophilic bifunctional polymeric resin (LS-2) with sulfonic groups was synthesized, and the adsorption performance of three aniline compounds, aniline, 4-methylaniline, and 4-nitroaniline onto LS-2 was compared with that on the commercial Amberlite XAD-4. The uptake of the aniline compounds on LS-2 is a procedure of coexistence of physisorption and chemisorption and obeys the pseudo-second order rate equation, while the uptake of the compounds on XAD-4 is merely a physical adsorption and follows the pseudo-first order rate equation. All the isothermal data fit well with the Freundlich model, and the values of K(F) of the compounds adsorbing on LS-2 are much higher than those on XAD-4 suggesting the higher adsorbing capacities on LS-2 than those on XAD-4, which may be attributed to the microporous structure and the polar groups on the network of LS-2 resin.

Adsorption characteristics of aniline and 4-methylaniline onto bifunctional polymeric adsorbent modified by sulfonic groups.

In this paper a new bifunctional polymeric resin (LS-2) was synthesized by introducing sulfonic groups onto the surface of the resin during the post-crossing of chloromethyl low crosslinking macroporous poly-styrene resin, and the comparison of the adsorption properties of LS-2 with Amberlite XAD-4 toward aniline and 4-methylaniline in aqueous solutions was made. The study focuses on the static equilibrium adsorption behaviors, the adsorption thermodynamics, and the column dynamic adsorption and desorption profiles. Freundlich model gives a perfect fitting to the isotherm data.

Supercritical carbon dioxide extraction of oil and squalene from amaranthus grain.

Supercritical carbon dioxide (SC CO(2)) was used for the extraction of oil and squalene from Amaranthus grain. Very small amounts of oil could be extracted by SC CO(2) from undisrupted grains, although SC CO(2) possesses higher diffusivity. Grinding increased the extraction rate and oil yield, and smaller particle size gave higher extraction rate.