Cupric ion coordination-mediated molecularly imprinted electrochemical sensor for the recognition and ratiometric detection of lidocaine.

Molecularly imprinted polymers (MIPs) have been widely used as artificial recognition elements in sensing applications. However, their electrochemical sensing performance is generally hampered by limited affinity and uncontrolled condition change. In this work, a novel MIP electrochemical sensor based on metal coordination interaction was prepared and used for the recognition and ratiometric detection of lidocaine (LC).

Novel ferrofluid based on water-based deep eutectic solvents: application in dispersive liquid-liquid microextraction of naphthalene-derived plant growth regulators in edible oil.

A new ferrofluid extractant (FeO @SiO-WDES) was ingeniously prepared by coating magnetic FeO @SiO microspheres with water-based deep eutectic solvent (WDES) and used for dispersive liquid-liquid microextraction (DLLME) and HPLC determination of naphthalene-derived plant growth regulators (PGRs, i.e., 1-naphthylacetic acid, 2-naphthylacetic acid, 1-naphthoxy acetic acid, 2-naphthyloxyacetic acid and 1-naphthylacetamide) in edible oil.

An effervescence-assisted microextraction for parabens in foodstuffs based on deep eutectic solvent composed of methyltrioctyl ammonium chloride and decanoic acid.

An efficient and ecofriendly effervescence-assisted emulsification microextraction approach based on hydrophobic deep eutectic solvent (EA-EME-DES) was developed for the sensitive chromatographic determination of parabens (i.e., methyl-, ethyl-, propyl- and butylparaben) in foodstuffs.

Molecular imprinting electrochemiluminescence sensor based on nitrogen-doped carbon quantum dots /Ru(bpy)@SiO for the determination of citrinin.

An electrochemiluminescence (ECL) sensor based on molecular imprinting polymer and SiO nanoparticles loaded Ru(bpy) and nitrogen-doped carbon quantum dots (NCQDs) is constructed for citrinin detection. The Ru(bpy) acts as ECL emitter, and the NCQDs cooperate with tri-n-propylamine (TPA) in solution as a coreactant to facilitate the luminescence. The citrinin imprinted poly(p-aminothiophenol) film is deposited on the surface of the luminophore by electrochemical method, which can immobilize the luminophore besides recognizing the target.

A ratiometric electrochemical sensing platform based on multifunctional molecularly imprinted polymer with catalytic activity for the detection of psychoactive substances.

Molecularly imprinted polymers (MIPs) are widely used as artificial recognition element in sensing field, but their electrochemical sensing performances are generally affected by their poor catalytic activity and unruly condition change. In this work, an MIP film with catalysis (Fe-DMMIP) is constructed by electrodeposition of Fe-coordinated aminophenanthroline and 3,4-ethylenedioxythiophene on N, S doped C material, using cannabinoid (CBD) as template molecule. Due to the presence of Fe-N active sites, the obtained Fe-DMMIP possesses enzyme-like catalytic activity besides conventional recognition capability.

A ratiometric electrochemical sensor based on Cu-coordinated molecularly imprinted polymer and porous carbon supported Ag nanoparticles for highly sensitive and selective detection of perphenazine.

In this work, a ratiometric electrochemical sensor was developed for the detection of perphenazine (PPZ). The sensor was constructed by electrodepositing Cu-coordinated molecularly imprinted polymer (Cu-MIP) on Ag nanoparticles (NPs) modified flexible porous carbon cloth. The Cu-MIP showed highly electrochemical response because of the enhanced adsorptive ability and electronic properties of Cu chelation; Ag NPs could provide a stable and effective reference signal for ratiometric quantification.

Organic-Inorganic Hybrid Flower-Shaped Microspheres Applied in Photoelectrochemical Sensing.

Organic-inorganic hybrid materials are rarely applied in photoelectrochemical (PEC) sensing because of the serious charge-carrier recombination in organic conjugated polymers. In this work, a series of poly(3,4-ethylenedioxythiophene) (PEDOT)/ZnInS hybrid flower-shaped microspheres were synthesized using ionic liquids (ILs) as the supporting electrolyte for EDOT electropolymerization and as the regulating reagent for controlling ZnInS growth, respectively. It was found that the hybrid material [HOEMIM]NTf-PEDOT/[HOEMIM]BF-ZnInS ([HOEMIM]: 1-hydroxyethyl-3-methylimidazolium cation; NTf: bis(trifluoromethanesulfonyl)amide) was the optimal one, with a smooth, transparent, and continuous polymer film covering the uniform and ordered cross-linked nanosheet arrays.

Water based-deep eutectic solvent for ultrasound-assisted liquid-liquid microextraction of parabens in edible oil.

A novel water-based deep eutectic solvent was synthesized and used for the ultrasound-assisted liquid-liquid microextraction of parabens in edible oil and for their determination by high performance liquid chromatography. Herein, the water-based deep eutectic solvent was formulated at room temperature by tetrabutylammonium chloride as hydrogen bond acceptor and water as hydrogen bond donor at the molar ratio of 1:5. As component, water has the effect on tailoring the physicochemical properties of water-based deep eutectic solvent and assisting tetrabutylammonium chloride (hydrogen bond acceptor) capturing parabens (hydrogen bond donor) through in-situ deep eutectic solvent formation.

Discovery of Natural Product-Based Fungicides (III): Semisynthesis and Biological Activity of N-Phenylpyrazole Sarisan Hybrids as Antifungal Agents.

Many phytopathogenic fungi can easily infect crops, resulting in crop yield reductions. In continuation of our efforts to develop natural product (NP)-based antifungal agents, a series of N-phenylpyrazole sarisan hybrids 6a-v were prepared via I -mediated oxidative cyclization, and their structures were determined by various spectral analyses including IR, H-NMR and ESI-MS. Among all N-phenylpyrazole sarisan hybrids, compounds 6a, 6b, 6e, 6i, 6j and 6r exhibited more encouraging antifungal action against at least two phytopathogenic fungi than the reference fungicide hymexazol.

Deep eutectic solvent-based liquid-phase microextraction coupled with reversed-phase high-performance liquid chromatography for determination of α-, β-, γ-, and δ-tocopherol in edible oils.

For simultaneous analysis of four fat-soluble tocopherols (α-, β-, γ-, and δ-) in edible oils, an efficient and green method using deep eutectic solvent-based liquid-phase microextraction (DES-LPME) coupled with reversed-phase high-performance liquid chromatography (RP-HPLC) was developed. The DESs formed by different quaternary ammonium salts and ethanol were used as the extractants. Tetrabutylammonium chloride (TBAC)-ethanol DES at a molar ratio of 1:2 achieved the best extraction efficiency.

Discovery of Natural Product-Based Fungicides (II): Semisynthesis and Biological Activity of Sarisan Attached 3-Phenylisoxazolines as Antifungal Agents.

Many phytopathogenic fungi cause severe damage to crop yields. In continuation of our research aimed at the discovery and development of natural products-based fungicides, a series of thirty-one sarisan attached 3-phenylisoxazolines were synthesized and evaluated for their antifungal activities against five phytopathogenic fungi (B. cinerea, C.

National Alveolar Echinococcosis Distribution - China, 2012-2016.

Both alveolar echinococcosis (AE) and cystic echinococcosis are endemic in China, among which alveolar echinococcosis has a very high mortality rate. The survey results showed the prevalence and scope of AE in China and identified high-risk groups including children, monks, herdsmen and illiterate people. At the same time, all the cases found in the survey (more than 90% of the patients did not go to the hospital for diagnosis and treatment before survey) were promptly diagnosed and treated.

[Construction a lentiviral vector for RNA interference of glutathione peroxidase 2 gene and its effect on HepG2 cell apoptosis].

Objective: To construct a RNA interference lentiviral vector for human glutathione peroxidase 2 (GPX2) gene and observe the effect of GPX2 knockdown on cell apoptosis.

Methods: The sequence of the small interfering RNA (siRNA) for GPX2 interference was inserted into the pSicoR vector. HepG2 cells were infected by the packaged si-GPX2 lentivirus and the expression of GPX2 in the infected cells was detected by both RT-PCR and Western blotting.

[Simultaneous determination of the migrations of bisphenol A and phenol in polycarbonate bottles based on subcritical water extraction and high performance liquid chromatography].

A new method was established for the simultaneous determination of the migration amounts of bisphenol A (BPA) and phenol from polycarbonate (PC) bottles based on subcritical water extraction (SWE) and high performance liquid chromatography. The optimum extraction conditions included an extraction temperature of 120 degree C, a pressure of 6.89 MPa (1000 psi), a static extraction time of 1 h and one cycle.

Rapid determination of α-tocopherol in cereal grains using dispersive liquid-liquid microextraction followed by HPLC.

The traditional for the determination of α-tocopherol in cereal grains includes saponification of a sample followed by liquid-liquid extraction, and it is time- and solvent consuming. In this study, a dispersive liquid-liquid microextraction (DLLME) method was developed to extract α-tocopherol in situ from the saponified grain sample solution. The DLLME experimental parameters including the type and volume of extractants, the volume of dispersers, the addition of salt and the extraction/centrifuging time were examined and optimized.

Formation of higher-order nuclear Rad51 structures is functionally linked to p21 expression and protection from DNA damage-induced apoptosis.

After exposure of mammalian cells to DNA damage, the endogenous Rad51 recombination protein is concentrated in multiple discrete foci, which are thought to represent nuclear domains for recombinational DNA repair. Overexpressed Rad51 protein forms foci and higher-order nuclear structures, even in the absence of DNA damage, in cells that do not undergo DNA replication synthesis. This correlates with increased expression of the cyclin-dependent kinase (Cdk) inhibitor p21.