Publications by authors named "Jiangnan Feng"
Autophagy is a major cellular pathway used to degrade long-lived proteins or organelles that may be damaged due to increased reactive oxygen species (ROS) generated by cellular stress. Autophagy typically enhances cell survival, but it may also act to promote cell death under certain conditions. The mechanism underlying this paradox, however, remains unclear.
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- - mTOR plays a significant role in promoting cancer cell growth and survival, making the mTOR inhibitor rapamycin a potential anti-cancer treatment, particularly at low doses where it suppresses a specific protein, S6 kinase.
- - High doses of rapamycin can induce apoptosis (cell death) in cancer cells, but the exact mechanisms behind this effect are not well understood; the study finds a link to the suppression of another protein, 4E-BP1, important for cancer cell survival.
- - The study also highlights that different responses to rapamycin are observed in various breast cancer cell lines, with some cells surviving due to changes in protein phosphorylation, indicating potential implications for tailored cancer therapies.
[Apoptosis of glioma cell line U251 induced by small interfering RNA targeting survivin].
Nan Fang Yi Ke Da Xue Xue Bao
April 2006
Objective: To construct recombinant expression vectors of small interfering RNA (siRNA) targeting survivin and investigate apoptosis of glioma cell line U251 mediated by the survivin-targeting siRNA.
Methods: According to the sequence of the coding region of survivin gene, two strings of 19 nucleotides of inverted sequence flanking the loop sequence of two complementary 9-base oligonucleotides were designed and synthesized to form hairpin construct as the DNA templates for the target siRNA. The siRNA templates were cloned into siRNA expression vector pGenesil-1, and the resulted vector pGenesil-1/survivin was transfected into U251 cells using Metafectene following the standard protocols.
Aim: To explore the possibility of repression of chloromycetin (Cm) acyl transferase by using external guided sequence (EGS) in order to converse the clinical E coli isolates from Cm- resistant to Cm- sensitive.
Methods: EGS directed against chloromycetin acetyl transferase gene (cat) was cloned to vector pEGFP-C1 which contains the kanamycin (Km) resistance gene. The recombinant plasmid pEGFP-C1+EGScat1+cat2 was constructed and the blank vector without EGS fragment was used as control plasmids.
Objective: To explore the possibility of phenotypic conversion of clinical chloromycetin (Cm)-resistant isolates of E.coli to drug-sensitive ones with external guide sequences (EGS) in vitro.
Methods: Recombinant EGS plasmids directed against Cm acetyl transferase (cat) and containing kanamycin (Km) drug-resistance gene and control plasmids only containing kanamycin-resistance gene without EGS were constructed.