LcMYB5, an R2R3-MYB family gene from Lonicera caerulea L., enhances drought and salt tolerance in transgenic tobacco and blue honeysuckle.

MYB transcription factors exert crucial functions in enhancing plant stress tolerance, which is impacted by soil drought and salinity. In our study, the R2R3-type MYB transcription factor gene LcMYB5 from blue honeysuckle (Lonicera caerulea L.) was successfully cloned and identified, and confirmed its nuclear localization.

Carvacrol enhances antioxidant activity and slows down cell wall metabolism by maintaining the energy level of 'Guifei' mango.

Background: Postharvest mango fruit are highly susceptible to rapid ripening, softening and senescence, greatly limiting their distribution. In this study, we evaluated the potential effects of carvacrol (0.06 g L) on mango (25 ± 1 °C) and the mechanisms by which it regulates antioxidant activity, energy and cell wall metabolism.

Microfluidic-blow-spinning of carvacrol-loaded porphyrin metal - organic framework nanofiber films with synergistic antibacterial capabilities for food packaging.

The adherence of foodborne microorganisms threatens human health, necessitating the development of antibacterial food packaging films. In this study, the antibacterial agent carvacrol (CV), hindered by its high volatility and intense aromatic odor, was encapsulated within the photosensitive metal-organic frameworks (MOFs) material PCN-224 (loading rate 50%). Subsequently, the microfluidic-blow-spinning (MBS) technique was employed for the rapid fabrication of CV@PCN-224/polycaprolactone (PCL)/chitosan (CS) nanofiber films.

Maintaining quality of postharvest green pepper fruit using melatonin by regulating membrane lipid metabolism and enhancing antioxidant capacity.

Green pepper quality often deteriorates during storage because of membrane lipid damage and oxidative stress. This study investigated the effects of exogenous melatonin (MT) on green pepper storage quality, membrane lipids, and antioxidant metabolism. The results showed that MT increased the activities of superoxide dismutase, catalase, ascorbate peroxidase, peroxidase, monodehydroascorbate reductase, and dehydroascorbate reductase in green peppers compared to the control group.

Investigating the impact of ultrasound on the structural, physicochemical, and emulsifying characteristics of Dioscorin: Insights from experimental data and molecular dynamics simulation.

This study investigated the impact of ultrasound treatment on dioscorin, the primary storage protein found in yam tubers. Three key factors, namely ultrasound power, duration, and frequency, were focused on. The research revealed that ultrasound-induced cavitation effects disrupted non-covalent bonds, resulting in a reduction in α-helix and β-sheet contents, decreased thermal stability, and a decrease in the apparent hydrodynamic diameter (Dh) of dioscorin.

Carboxymethyl chitosan and polycaprolactone-based rapid in-situ packaging for fruit preservation by solution blow spinning.

Nanofiber packaging has not yet gained practical application in fruit preservation because of some limitations, such as low production rate and utilization, and failure due to poor adhesion to the fruit. Herein, to solve this issue, a novel fruit packaging method based on solution blow spinning (SBS), called in-situ packaging, was pioneered. Specifically, carboxymethyl chitosan (CMCH) and polycaprolactone (PCL) were chosen as substrate materials and cherry tomatoes were selected as demonstration subjects.

Photocatalytic titanium dioxide reduces postharvest decay of nectarine fruit packaged in different materials through modulating central carbon and energy metabolisms.

The capacity of titanium dioxide (TiO) photocatalysis photocatalytic reactor to prevent and control pathogen infection in nectarine fruit packed in laminated nylon/LDPE, low density polyethylene and microperforated LDPE films was evaluated. Results showed that TiO combined with microperforated LDPE packaging (TPL) exhibited superior inhibition of microbial growth, reducing total viable counts by 4.18 log CFU g and yeast and mold counts by 3.

Revealing the mechanism underlying the effects of γ-aminobutyric acid-dioscorin interactions on dioscorin structure and emulsifying properties by molecular dynamic simulations.

Many studies have shown that γ-aminobutyric acid (GABA) exhibits various beneficial biological activities, including gut-modulating, neuro-stimulating, and cardio-protecting activities. Naturally, GABA exists in small amounts in yam, which is primarily synthesized by the decarboxylation of L-glutamic acid in the presence of glutamate decarboxylase. Dioscorin, the major tuber storage protein of yam, has been shown to have good solubility and emulsifying activity.

Effect of Controlled Atmosphere Packaging on the Physiology and Quality of Fresh-Cut .

is a typical edible fungus of Guizhou Province and is very popular due to its unique taste and texture. In this study, the effect of a controlled atmosphere (CA) on fresh-cut shelf life was investigated. Firstly, this study addresses the influence of different O concentrations (5%, 20%, 35%, 50%, 65%, 80%, or 95%) with N balance on fresh-cut quality while stored at 4 ± 1 °C for 7 d.

Effects of spray drying and freeze drying on the structure and emulsifying properties of yam soluble protein: A study by experiment and molecular dynamics simulation.

This study focused on the effects of freeze drying (FD) and sprays drying (SD) on the structure and emulsifying properties of yam soluble protein (YSP). The results showed that the surface hydrophobicity (Ho) value, free sulfhydryl group (SH) content, turns content, denaturation temperature and enthalpy value of spray-dried YSP (SD-YSP) were higher than freeze-dried YSP (FD-YSP), but the apparent hydrodynamic diameter (Dh) value of SD-YSP was smaller. The smaller Dh, higher Ho and free SH led to higher percentage of adsorbed proteins and stronger binding between protein and oil droplet in emulsions.

Chilling injury of tomato fruit was alleviated under low-temperature storage by silencing Sly-miR171e with short tandem target mimic technology.

In this study, the role of Sly-miR171e on post-harvest cold tolerance of tomato fruit was researched. The results showed that overexpression of Sly-miR171e (miR171e-OE) promoted postharvest chilling injury (CI) of tomato fruit at the mature red (MR) and mature green (MG) stage. Contrasted with the wild type (WT) and miR171e-OE fruit, the knockdown of Sly-miR171e (miR171e-STTM) showed a lower CI index, lower hydrogen peroxide (HO) content, and higher fruit firmness after harvest.

Melatonin maintained higher contents of unsaturated fatty acid and cell membrane structure integrity in banana peel and alleviated postharvest chilling injury.

Exogenous melatonin confers the chilling tolerance of banana fruit by promoting reactive oxygen species (ROS) scavenging system and inducing the unsaturated fatty acid synthesis. The results showed that melatonin treatment increased the contents of phospholipids, promoted the ROS scavenging enzyme, and restrained the activities of lipoxygenase (LOX), and thus reduced the lipid peroxidation of banana peel. In addition, melatonin treatment increased the flavonoids and proline contents, which was conducive to antioxidant capacity.

Preparation, Shelf, and Eating Quality of Ready-to-Eat "Guichang" Kiwifruit: Regulation by Ethylene and 1-MCP.

The acceptance of kiwifruit by consumers is significantly affected by its slow ripening and susceptibility to deterioration. Ready-to-eat "Guichang" kiwifruit and its preparation technology were studied by the regulation of ethylene and 1-MCP. Harvested kiwifruits were treated with 100-2000 μl Lethylene for 36 h (20°C) and then treatment with 0-0.

Cell wall polysaccharides and mono-/disaccharides as chemical determinants for the texture and hygroscopicity of freeze-dried fruit and vegetable cubes.

In order to select the critical factors on the texture and hygroscopic characteristics of freeze-dried fruit and vegetable cubes, the correlation analysis was performed on the major chemical compositions of 12 fresh materials, and the microstructure, texture, hygroscopicity of corresponding freeze-dried samples. The dry proportion of starch-rich materials, such as taro, was mainly composed of polysaccharides (0.76-0.

Facile microfluidic fabrication and characterization of ethyl cellulose/PVP films with neatly arranged fibers.

Much attention and endeavor have been paid to developing biocompatible food packaging films. Here, ethyl cellulose (EC) and polyvinylpyrrolidone (PVP) were fabricated into films through a facile method, microfluidic spinning. Morphology observations showed that the fibers were neatly arranged with an average diameter of 1-4 μm.

Effects of Ozone Fumigation on the Main Postharvest Pathogenic Fungi sp. and the Storage Quality of Blueberry in Majiang County, China.

In this study, the fungus sp. was isolated from rotting postharvest blueberry fruits at different storage stages and identified into genera. Inoculation of this strain on the surface of fresh fruits was able to cause rotting.

Chitosan/PCL nanofibrous films developed by SBS to encapsulate thymol/HPβCD inclusion complexes for fruit packaging.

In this work, the solution blow spinning (SBS) technique was used to rapidly fabricate the thymol (THY)/2-hydroxypropyl-β-cyclodextrin (HPβCD) inclusion complexes loaded chitosan (CS)/polycaprolactone (PCL) nanofibrous films for fruit preservation and packaging. XRD results indicated that the THY/HPβCD inclusion complexes were successfully incorporated into the CS/PCL nanofibers. The nanofibrous films had an increase of average diameters of nanofibers from 243.

Effects of inside-out heat-shock via microwave on the fruit softening and quality of persimmon during postharvest storage.

Rapid postharvest softening largely limits the shelf-life of persimmon (Diospyros kaki L.) fruit. Microwave is a new environmental-friendly inside-out heat-shock approach, whose effect on the fruit softening and quality has not yet been investigated.

Dynamic Changes of DNA Methylation Induced by Heat Treatment Were Involved in Ethylene Signal Transmission and Delayed the Postharvest Ripening of Tomato Fruit.

Deoxyribonucleic acid (DNA) methylation plays an important role in fruit ripening and senescence. Here, the role of DNA methylation of the CpG island of , , , , and genes induced by heat treatment (37 °C) in postharvest ripening of tomato fruit was studied. After heat treatment, the firmness and vitamin C content showed higher levels, the loss of aldehydes in volatile components was delayed, and the activities of methylase and demethylase decreased in tomato fruit.

Combination of Low Fluctuation of Temperature with TiO Photocatalytic/Ozone for the Quality Maintenance of Postharvest Peach.

Chilling injury, tissue browning, and fungal infection are the major problems of peach fruit during post-harvest storage. In this study, a precise temperature control cold storage with low-temperature fluctuation (LFT) and internal circulation flow system is designed. An ozone (O) generator and a (titanium dioxide) TiO photocatalytic reactor were applied to cold storage to investigate the variation of LFT combined with ozone fumigation and a TiO photocatalytic reactor in the efficiency of delaying ripening and maintaining peach fruit quality.

Antifungal activity of 1-methylcyclopropene (1-MCP) against anthracnose (Colletotrichum gloeosporioides) in postharvest mango fruit and its possible mechanisms of action.

Anthracnose caused by Colletotrichum gloeosporioides is one of the most important postharvest diseases in mango fruit, often causing huge economic losses. In this study, the effect of 1-methylcyclopropene (1-MCP) against anthracnose in postharvest mango fruit and the mechanisms involved were investigated. 1-MCP induced reactive oxygen species (ROS) generation, damaged the mitochondria and destroyed the integrity of plasma membrane of spores of C.

Effects of ripening, 1-methylcyclopropene and ultra-high-pressure pasteurisation on the change of volatiles in Chinese pear cultivars.

Background: Aroma is one important fruit sensory attribute influenced by the volatile constituents related to species, variety and technological treatments. We analysed the variations of volatile compounds in five pear cultivars and investigated their changes related to different pear organs, different ripening stages, 1-MCP treatment and ultra-high-pressure pasteurisation.

Results: Considerable variations exist in the quantity of 10 volatile compounds among five pear cultivars.

[Research on nondestructive measurement of soluble tannin content of astringent persimmon using visible and near infrared diffuse reflection spectroscopy].

The objectives of the present study were to establish the relationships of the visible and near infrared diffuse reflection (Vis/NIR) spectroscopy and the soluble tannin content of internal quality index of astringent persimmon, and to evaluate the value of Vis/NIR spectroscopy in measuring the soluble tannin content of internal quality index of astringent persimmon. In the spectral region between 570 and 1 848 nm, calibration results for the soluble tannin content of astringent persimmon were compared with different regression techniques, different derivative treatments and different scatter and standard treatments. The results showed that the modified partial least squares(MPLS) model, with respect to the first derivative D1 log(1/R) and detrend only, provided better prediction performance for the soluble tannin content of astringent persimmon fruit, with the correlation coefficient of cross validation of calibration (R(cv)) and correlation coefficient of prediction (R(p2)), the root mean square error of cross validation of calibration (RMSECV) and the root mean square error of prediction (RMSEP) of 0.