Publications by authors named "Jianghua Zhan"
Article Synopsis
- This study aimed to use a two-photon microscope excitation Airy beam to create detailed 3D images of intrahepatic bile ducts in mice with bile duct ligation (BDL) and human patients with biliary atresia (BA).
- Ten BDL mice showed symptoms like jaundice and weight loss, with liver tissues sampled after 10 days, while 16 BA patients underwent either liver transplantation or Kasai portoenterostomy (KPE).
- The imaging revealed significant bile duct widening and hyperplasia in BDL mice and disorganized bile ducts in BA patients, particularly those who had liver transplants.
Kasai procedure or liver transplantation: how should we choose in biliary atresia?
Hepatobiliary Surg Nutr
December 2024
Background: Ectopic thyroid is a rare disease, with ectopic thyroid in the porta hepatis being extremely rare. We present a rare case involving an infant with biliary atresia (BA) wherein ectopic thyroid tissue was incidentally discovered in the porta hepatis after the Kasai operation.
Case Description: A 2-month-old boy initially presented with jaundice, manifesting as yellow skin and sclera, on the first day following birth.
Article Synopsis
- - Biliary atresia (BA) is a serious liver condition that worsens without prompt surgery; the Kasai portoenterostomy (KPE) is a common surgical option to improve bile flow, with varying success rates based on the surgical method used (laparoscopic, robot-assisted, or open).
- - A comprehensive review of existing studies identified no significant differences in the postoperative outcomes of the three surgical techniques, although robot-assisted KPE showed slightly better results compared to open and laparoscopic methods concerning successful clearance of jaundice.
- - The choice of surgical technique should be personalized based on the patient's circumstances and potential costs, emphasizing the importance of effective postoperative care to minimize complications.
Article Synopsis
- - The study aimed to review past research conducted by the team on the mechanisms and evaluation of liver fibrosis in biliary atresia (BA) and to outline future research directions.
- - A total of 58 articles were analyzed, revealing 25 focused on the mechanisms of liver fibrosis in BA, while five examined its assessment, leading to the introduction of key pathways and a new grading system for liver fibrosis.
- - The proposed grading system is intended to enhance the accuracy of evaluating children's conditions and treatment plans, with a focus on the TGF-β1 signaling pathway as crucial, while also calling for more research on epithelial-mesenchymal transition (EMT) in BA.
Background: Acral persistent papular mucinosis (APPM) is a rare idiopathic subtype of localized lichen myxedematosus. To date, there have been less than 41 APPM cases reported worldwide, however, almost all patients were older than 18 years of age. A 7-year-old child was first reported in this paper.
View Article and Find Full Text PDFBackground: Systemic Immune-Inflammation Index (SII), known as an easy, economical and useful marker, correlates with the balance of inflammation and immune response. However, the usefulness of SII in biliary atresia (BA) remains unclear. Therefore, we evaluated the relationship of SII level and postoperative clinical outcomes of BA.
View Article and Find Full Text PDFBackground: Biliary atresia (BA), a progressive condition affecting canalicular-bile duct function/anatomy, requires prompt surgical intervention for favorable outcomes. Therefore, we conducted a network meta-analysis of common diagnostic methods to assess their performance and provide evidence-based support for clinical decision-making.
Methods: We reviewed literature in PubMed, EMBASE, and Cochrane for BA diagnostics.
Background: Acute complete gastric volvulus is a rare and life-threatening disease, which is prone to gastric wall ischemia, perforation, and necrosis. If it is not treated by surgery in time, the mortality rate can range from 30 to 50%. Clinical presentations of acute gastric volvulus are atypical and often mimic other abdominal conditions such as gastritis, gastroesophageal reflux, gastric dilation, and pancreatitis.
View Article and Find Full Text PDFBackground: In recent years, Mendelian randomization (MR) has been widely used to infer causality of related disease risk exposures. However, this strategy has not been applied to biliary atresia (BA).
Methods: Genome-wide association studies (GWAS) data of 41 inflammatory cytokines, 731 immune cell traits, and 1400 metabolites were obtained from public databases as exposure factors.
Background: Kasai procedure and liver transplantation are effective ways to save the life of children with biliary atresia (BA). However, with the gradual development of liver transplantation technology, scholars have questioned the necessity of the Kasai procedure. Therefore, we conducted a meta-analysis to evaluate the effect of previous Kasai procedures on liver transplantation in children with BA.
View Article and Find Full Text PDFBiliary atresia is an occlusive biliary disease involving intrahepatic and extrahepatic bile ducts. Its etiology and pathogenesis are unclear. There are many manifestations of bile duct involvement in biliary atresia, but little is known about its occurrence and development.
View Article and Find Full Text PDFPurpose: To determine the prevalent microbiological profile of biliary atresia (BA) patients at the time of its occurrence by studying their intestinal flora.
Methods: A total of 118 gut microbiota samples from three groups of 43 BA patients, 33 disease controls (DC) with other cholestatic diseases and 42 healthy controls (HC), were analyzed by deep mining of public data. Subsequently, a total of 23 fecal samples from three groups of clinically collected patients (11 BA, 6 DC and 6 HC) were sequenced for 16S rRNA gene amplification and analyzed for serum butyrate (BU) level by liquid chromatography.
Objectives: To validate an appropriate evaluation method of liver fibrosis assessment based on the unique pathological features of biliary atresia (BA) that could well predict its prognosis.
Methods: A total of 68 patients with BA who underwent Kasai procedure (KP) and an intraoperative liver biopsy, followed up from January 2019 to December 2021, were recruited in a retrospective analysis. Ishak, Metavir, and BA-specific staging systems in relation to outcomes were analyzed using logistic regression, COX proportional hazard regression, Kaplan-Meier analysis, etc.
Our purpose of this study was to explore the application effect of respiratory flora regulation in bronchiolitis obliterans after lung transplantation, and its regulatory effect on the microbial environment of the lesion and the IL-10/STAT3 signaling pathway. 25 clean-grade C57BL/6 male mice and 5 BALB/c male mice were selected for orthotopic tracheal transplantation and postoperative respiratory flora regulation in a hospital animal room from Jan 2019 to Dec 2021. Next, the changes in the microbial environment and the IL-10/STAT3 signaling pathway before and after respiratory flora regulation were compared, so as to evaluate the regulatory effect of this method.
View Article and Find Full Text PDFArticle Synopsis
- Cholangitis is a common complication in patients who have undergone Kasai portoenterostomy (KPE) for biliary atresia, and timely treatment with antibiotics is critical due to uncertain bacterial infection.
- This study included a multicenter, randomized trial where patients with different severities of cholangitis were treated with various combinations of antibiotics, focusing primarily on the effectiveness of cefoperazone sodium tazobactam sodium (CSTS) and meropenem (MEPM).
- Results showed no significant differences in outcomes like fever duration or liver function recovery between CSTS and MEPM in mild cases, but severe cases benefitted from MEPM combined with immunoglobulin (IVIG), reducing fever duration and improving
Importance: Multiple studies indicate a possible correlation between ADD3 rs2501577 and biliary atresia susceptibility; however, a conclusive determination has yet to be made.
Objective: Investigate the role of ADD3 rs2501577 in biliary atresia susceptibility across diverse populations.
Data Sources: The study protocol has been registered on PROSPERO, an international platform for systematic review registration (PROSPERO ID: CRD42023384641).
Background: The pathogenesis of liver fibrosis in biliary atresia (BA) is unclear. Epidermal growth factor (EGF) plays a vital role in liver fibrosis. This study aims to investigate the expression of EGF and the mechanisms of its pro-fibrotic effects in BA.
View Article and Find Full Text PDFPurpose: Metagenomic next-generation sequencing (mNGS) is an emerging technique for pathogen detection. However, most literature on the clinical application of pediatrics generally comprises case reports or small-scale cohort studies.
Patients And Methods: A total of 101 children with community-acquired severe pneumonia admitted to Tianjin Children's Hospital from November 2021 to February 2022 were included.
Background: Rapid and accurate identification of pathogens is very important for the treatment of Severe community-acquired pneumonia (SCAP) in children. Metagenomic Next-generation sequencing (mNGS) has been applied in the detection of pathogenic bacteria in recent years, while the overall evaluation the application of SCAP in children is lacking.
Methods: In our study, 84 cases of SCAP were enrolled.
Background And Purpose: If the preoperative pathological information is inadequate, a risk classification may not be able to be determined for some patients with neuroblastoma. Our objectives were to include imaging factors, serum biomarkers, and demographic factors in a nomogram to distinguish high-risk patients before surgical resection based on the COG classification.
Method: A total of 106 patients were included in the study.
Purpose: The efficacy of robot-assisted hepaticojejunostomy (RAHJ) and laparoscopic-assisted hepaticojejunostomy (LAHJ) in children with congenital choledochal dilatation has been a topic of much debate and controversy. The purpose of this study was to evaluate the role of RAHJ and LAHJ in pediatric congenital choledochal dilatation.
Method: The review program has been prospectively registered (PROSPEROID: CRD42022306868).
Purpose: Based on a public gene expression database, this study established the immune-related genetic model that distinguished BA from other cholestasis diseases (DC) for the first time. We explored the molecular mechanism of BA based on the gene model.
Methods: The BA microarray dataset GSE46960, containing BA, other cause of intrahepatic cholestasis than biliary atresia and normal liver gene expression data, was downloaded from the Gene Expression Omnibus (GEO) database.
Purpose: The purpose of our study is to identify potential biomarkers of hepatoblastoma (HB) and further explore the pathogenesis of it.
Methods: Differentially expressed genes (DEGs) were incorporated into the combined random forest and artificial neural network diagnosis model to screen candidate genes for HB. Gene set enrichment analysis (GSEA) was used to analyze the ARHGEF2.