Development and validation of a highly rapid and sensitive LC-MS/MS method for determination of SZ-685C, an investigational marine anticancer agent, in rat plasma--application to a pharmacokinetic study in rats.

A sensitive and rapid method was developed and validated for the quantitative analysis of the novel anticancer agent SZ-685C in rat plasma using high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS) in negative ion mode in order to support the following pre-clinical and clinical studies. SZ-685C and the internal standard (IS, emodin) were extracted from rat plasma by a simple liquid-liquid extraction technique using ethyl acetate as extraction solvent. Chromatographic separation was performed on an Elite Hypersil BDS C18 column (100 mm × 2.

[Comparison and analysis of the molecular character of breakpoints in introns of deletion hotspots of dystrophin gene].

Objective: To study the exons deletion mechanisms for dystrophin gene, the molecular characters of breakpoints of junction fragments for deletion-type Duchenne muscular dystrophy (DMD) patients with 46 and 51 exons deletion were compared and analyzed.

Methods: Deletion-type DMD patients were detected by multiplex polymerase chain reaction(mPCR). The breakpoints of junction fragments with 46 and 51 exons deletions were cloned and sequenced respectively.

Analytical solutions of simply supported magnetoelectroelastic circular plate under uniform loads.

In this paper, the axisymmetric general solutions of transversely isotropic magnetoelectroelastic media are expressed with four harmonic displacement functions at first. Then, based on the solutions, the analytical three-dimensional solutions are provided for a simply supported magnetoelectroelastic circular plate subjected to uniform loads. Finally, the example of circular plate is presented.