Heat Diffusion Kernel Algorithm-Based Interpretation of the Disease Intervention Mechanism for DHA.

Docosahexaenoic acid (DHA) is effective in the prevention and treatment of cancer, congenital disorders, and various chronic diseases. According to the omnigenic hypothesis, these complex diseases are caused by disordered gene regulatory networks comprising dozens to hundreds of core genes and a mass of peripheral genes. However, conventional research on the disease intervention mechanism of DHA only focused on specific types of genes or pathways instead of examining genes at the network level, resulting in conflicting conclusions.

Identifying cancer prognostic modules by module network analysis.

Background: The identification of prognostic genes that can distinguish the prognostic risks of cancer patients remains a significant challenge. Previous works have proven that functional gene sets were more reliable for this task than the gene signature. However, few works have considered the cross-talk among functional gene sets, which may result in neglecting important prognostic gene sets for cancer.

The effects of secretion factors from umbilical cord derived mesenchymal stem cells on osteogenic differentiation of mesenchymal stem cells.

Factors synthesized by mesenchymal stem cells (MSCs) contain various growth factors, cytokines, exosomes and microRNAs, which may affect the differentiation abilities of MSCs. In the present study, we investigated the effects of secretion factors of human umbilical cord derived mesenchymal stem cells (hUCMSCs) on osteogenesis of human bone marrow derived MSCs (hBMSCs). The results showed that 20 μg/ml hUCMSCs secretion factors could initiate osteogenic differentiation of hBMSCs without osteogenic induction medium (OIM), and the amount of calcium deposit (stained by Alizarin Red) was significantly increased after the hUCMSCs secretion factors treatment.

MDAS: an integrated system for metabonomic data analysis.

Metabonomics, the latest 'omics' research field, shows great promise as a tool in biomarker discovery, drug efficacy and toxicity analysis, disease diagnosis and prognosis. One of the major challenges now facing researchers is how to process this data to yield useful information about a biological system, e.g.

Real-Time, Automatic Detection Of A Small Biomolecule Using The Novel Portable On-line Biomolecules Analyzer.

Previous studies have shown that combined stresses in space flight can affect astronauts' health. On-line monitoring biochemical markers in body fluids which are highly related to some kinds of diseases is a practical method used as the health early alarm and medical care system for astronauts. In this study, a portable on-line biomolecules analyzer (POBA) was developed and utilized for the assay of 2,4-Dinitrophenylhydrazine(DNPH), which offered a portable model for the measurement of low-molecular-weight biochemical markers in body fluids at real-time.

[Detection of small molecules based on surface plasmon (correction of plasman) resonance technology].

Objective: To develop a detective method applied in online assaying of astronauts' humours using the portable online bio-molecules analyzer (POBA) based on surface plasmon resonance biosensor.

Method: An assay format was developed based on the detection of 2, 4-Dinitrophenyl-hydrazine. The bio-molecule slide was made by DNP-BSA.

Gray characteristic analysis of microtubules in cardiac myocytes.

Objective: To quantify the images of the microtubules in fetal rat cardiac myocytes under simulated microgravity by utilizing the characteristic parameters of image gray, and to study their morphological change.

Method: Gray characteristic of the microtubules in fetal rat cardiac myocytes was quantified in both simulated microgravity and control conditions by variance, skewness, and kurtosis.

Result: From feature analysis of 24 images, the characteristic parameters selected here were proved to be effective.

[Image analysis of cardiac muscle cytoskeleton under simulated microgravity based on gray-level co-occurrence matrix (GLCM)].

Objective: To study morphological changes of the cytoskeleton-microtubule (MT) of the fetal rat cardiac myocytes under simulated microgravity, and to quantify its image by utilizing the gray level co-occurrence matrix (GLCM) parameters of the image.

Method: Cytoskeleton images, including cellular microphotographs taken under normal or microgravity (clinostat) conditions, were quantified by gray level co-occurrence matrix parameters, and the pharmacological counter effect of quercetin against the influences of microgravity was estimated with these parameters.

Result: The results showed that the texture of microtubules in the image became worse under simulated microgravity environment.

[Effects of nitric oxide on myocardial contraction function].

Nitric oxide (NO) produced by myocardial nitric oxide synthase has been implicated as a modulator of myocardial contraction [correction of contracion]. This paper reviewed the reports on myocardial contraction modulated by NO, its mechanism, and regulation of expression and activity of iNOS. NO was recently shown to produce biphasic contractile [correction of contratile] effects on myocardium: augmentation at low levels and depression at high levels.