Depth and frequency dependence of geoacoustic properties on the New England Mud Patch from reflection coefficient inversiona).

Muddy sediments cover significant portions of continental shelves, but their physical properties remain poorly understood compared to sandy sediments. This paper presents a generally applicable model for sediment-column structure and variability on the New England Mud Patch (NEMP), based on trans-dimensional Bayesian inversion of wide-angle, broadband reflection-coefficient data in this work and in two previously published reflection-coefficient inversions at different sites on the NEMP. The data considered here include higher frequencies and larger bandwidth and cover lower reflection grazing angles than the previous studies, hence, resulting in geoacoustic profiles with significantly better structural resolution and smaller uncertainties.

Number of Stent Retriever Passes Associated with Clinical Outcome After Mechanical Thrombectomy in Acute Ischemic Stroke.

Objective: To evaluate the association between the number of stent retriever (SR) passes and clinical outcome after mechanical thrombectomy (MT) in patients with acute ischemic stroke(AIS).

Methods: We retrospectively analyze data collected from consecutive patients with large vessel occlusion (LVO) in anterior circulation treated with MT. Baseline characteristics, number of SR passes, symptomatic intracranial hemorrhage (sICH), clinical outcome measured by modified Rankin Scale (mRS) at 90 days after MT were collected.

The Value of the Score for the Targeting of Atrial Fibrillation (STAF) Screening in Acute Stroke Patients.

Background And Purpose: Recently, the score for the targeting of atrial fibrillation (STAF) was introduced to identify the risk of atrial fibrillation (AF) in stroke patients. In this study, we aim to evaluate the usefulness of the STAF score for AF screening in acute stroke patients.

Methods: Patients with acute ischemic stroke who were admitted to our stroke unit were prospectively enrolled from March 2011 to March 2013.

The impact of ocean sound speed variability on the uncertainty of geoacoustic parameter estimates.

This paper investigates the influence of water column variability on the estimates of geoacoustic model parameters obtained from matched field inversions. The acoustic data were collected on the New Jersey continental shelf during shallow water experiments in August 2006. The oceanographic variability was evident when the data were recorded.

Estimating marine sediment attenuation at low frequency with a vertical line array.

This paper presents a method of determining the compressional wave attenuation in marine sediment from a short range measurement. The data were collected on a vertical line array at a range of 230 m during the Shallow Water 2006 experiments. The sediment attenuation is extracted from the signal strength ratio of the sea bottom reflection to a sub-bottom reflection at different frequencies from 1.

Calcium and cyclic nucleotides affect TNF-alpha-induced stem cell migration.

The purpose of this study was to study the effect of calcium, cyclic AMP (cAMP) and cyclic GMP (cGMP) on embryonic stem cell (ESC) motility during TNF-alpha-induced chemotaxis. ESCs were monitored using a chemotaxis chamber, with different concentrations of calcium or cAMP or cGMP added to the medium. Changes in intracellular calcium ([Ca(2+)](i)) were measured with the fluorescent dye fura-2/AM.

Numerical investigation of out-of-plane sound propagation in a shallow water experiment.

In an experiment in the Florida Straits, broadband pulses were transmitted over a range of 10 km and received by a vertical hydrophone array. For pulses with center frequency below 400 Hz, the received signal consisted of a dominant arrival followed by a secondary one delayed by about 0.4 s.

Short range travel time geoacoustic inversion with vertical line array.

This paper presents travel time geoacoustic inversion of broadband data collected on a vertical line array at short range of 230 m during the Shallow Water 2006 experiments. A ray-tracing method combined with a hybrid optimization algorithm that utilizes differential evolution and downhill simplex was used for the inversion of sediment properties. The ocean sound speed profile and geometric parameters were inverted prior to the sea bottom properties to account for the temporally variable ocean environment.

Clozapine-induced myocarditis: role of catecholamines in a murine model.

Clozapine, an atypical antipsychotic, is very effective in the treatment of resistant schizophrenia. However, cardiotoxicity of clozapine, particularly in young patients, has raised concerns about its safety. Increased catecholamines have been postulated to trigger an inflammatory response resulting in myocarditis, dilated cardiomyopathy, and death, although this has not yet been thoroughly studied.

Bayesian geoacoustic inversion in a dynamic shallow water environment.

This paper presents results for matched field Bayesian geoacoustic inversion of multitonal continuous wave data collected on the New Jersey continental shelf. To account for effects of significant spatial and temporal variation of the water column sound speed, the sound speed profile was represented by empirical orthogonal functions. Data error information for the inversion was estimated from multiple time windows of the data.

Quantifying the uncertainty of geoacoustic parameter estimates for the New Jersey shelf by inverting air gun data.

This paper describes geoacoustic inversion of low frequency air gun data acquired during an experiment on the New Jersey shelf. Hybrid optimization and Bayesian inversion techniques based on matched field processing were applied to multiple shots from three air gun data sets recorded by a vertical line array in a long-range shallow water geometry. For the Bayesian inversions, full data error covariance matrix was estimated from a set of consecutive shots that had high temporal coherence and small spatial variation in source position.

Antiapoptotic effect of implanted embryonic stem cell-derived early-differentiated cells in aging rats after myocardial infarction.

This study tested whether implanted embryonic stem cell-derived early-differentiated cells (EDCs) lead to improvement in cardiac function by preventing cardiac apoptosis in aging rats after myocardial infarction. Cardiac apoptosis after transplantation of EDCs was assessed in situ by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling reaction (TUNEL) staining as well as by measurements of protein levels of cleaved caspases 3, Bax, and Bcl-2. Our results indicate that cell transplantation improved cardiac function at 6-months observation.

Successful implantation of intravenously administered stem cells correlates with severity of inflammation in murine myocarditis.

The present study was designed to determine whether cardiac inflammation is important for the successful homing of stem cells to the heart after intravenous injection in a murine myocarditis model. Male Bagg albino/c mice were infected with encephalomyocarditis virus (EMCV) to produce myocarditis. Subgroups of mice received single injections by tail vein of embryonic stem cells (ESCs) transfected with green fluorescent protein (GFP) as a marker at days 3, 14, or 60 after infection; other subgroups without stem cell injections were killed at each of these time points to assess the degree of inflammation present.

Vascular endothelial growth factor promotes cardiomyocyte differentiation of embryonic stem cells.

Embryonic stem cells (ESCs) overexpressing the vascular endothelial growth factor (VEGF) improve cardiac function in mouse models of myocardial ischemia and infarction by mechanisms that are poorly understood. Here we studied the effects of VEGF on cardiomyocyte differentiation of mouse ESCs in vitro. We used flow cytometry to determine the expression of alpha-myosin heavy chain (alpha-MHC), cardiac troponin I (cTn-I), and Nkx2.

Homing of intravenously infused embryonic stem cell-derived cells to injured hearts after myocardial infarction.

Objective: The present study was designed to test whether intravenously infused embryonic stem cell-derived cells could translocate to injured myocardium after myocardial infarction and improve cardiac function.

Methods: Cultured embryonic stem cell-derived cells were transfected with green fluorescent protein. Embryonic stem cell-derived cells were administered through the tail vein (approximately 10(7) cells in 1 mL of medium for each rat) every other day for 6 days in 45 rats after myocardial infarction.

Targeted deletion of Puma attenuates cardiomyocyte death and improves cardiac function during ischemia-reperfusion.

The p53-upregulated modulator of apoptosis (Puma), a BH3-only member of the Bcl-2 protein family, is required for p53-dependent and -independent forms of apoptosis and has been implicated in the pathomechanism of several diseases, including cancer, acquired immunodeficiency syndrome, and ischemic brain disease. The role of Puma in cardiomyocyte death, however, has not been analyzed. On the basis of the ability of Puma to integrate diverse cell death stimuli, we hypothesized that Puma might be critical for cardiomyocyte death upon ischemia-reperfusion (I/R) of the heart.

Stem cell therapy in the aging hearts of Fisher 344 rats: synergistic effects on myogenesis and angiogenesis.

Objective: Advanced age is a major risk factor for ventricular dysfunction and reduction of cardiac reserve. Finding novel approaches to prevent and attenuate heart dysfunction associated with advanced age is a major therapeutic challenge. The present study was designed to test whether engrafted embryonic stem cells could improve myocardial function in aging hearts.

Cocaine and catecholamines enhance inflammatory cell retention in the coronary circulation of mice by upregulation of adhesion molecules.

Cocaine treatment of mice with viral myocarditis significantly increases neutrophil infiltration into the myocardium and exacerbates the inflammatory response. The mechanisms of these effects are unknown; however, it may be that cocaine increases circulating catecholamines and consequently increases inflammatory cell adhesion to the coronary endothelium. Here, we examined the hypothesis that cocaine enhances inflammatory cell infiltration via catecholamine-induced upregulation of cell adhesion molecule (CAM) expression in adult BALB/c mouse hearts.

Immunosuppression and xenotransplantation of cells for cardiac repair.

The death of highly vulnerable cardiomyocytes during ischemia leads to cardiac dysfunction, including heart failure. Due to limited proliferation of adult mammalian cardiomyocytes, the dead myocardium is replaced by noncontractile fibrotic tissue. Introducing exogenous cells to participate in the regeneration of infarcted myocardium has thus been proposed as a novel therapeutic approach.

Dantrolene: effects on abnormal intracellular Ca(2+) handling and inotropy in postinfarcted rat myocardium.

The present study was designed to investigate the effects of dantrolene on intracellular Ca(2+) ([Ca(2+)](i)) handling and inotropy in rat infarcted myocardium. Dantrolene-treated rats with myocardial infarction were placed into two different dosage groups. The infarcted control group received placebo only.

Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia.

Preeclampsia, a syndrome affecting 5% of pregnancies, causes substantial maternal and fetal morbidity and mortality. The pathophysiology of preeclampsia remains largely unknown. It has been hypothesized that placental ischemia is an early event, leading to placental production of a soluble factor or factors that cause maternal endothelial dysfunction, resulting in the clinical findings of hypertension, proteinuria, and edema.

Long-term improvement of cardiac function in rats after infarction by transplantation of embryonic stem cells.

Objective: This study was designed to investigate the feasibility of and potential functional improvement with embryonic stem cell transplantation in rats 32 weeks after myocardial infarction.

Methods: Before cell transplantation, cultured embryonic stem cells were transfected with the complementary DNA of green fluorescent protein to identify engrafted cells in myocardium. Myocardial infarction was induced by ligation of the left coronary artery.

Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs.

Background: Viable cardiomyocytes after myocardial infarction (MI) are unable to repair the necrotic myocardium due to their limited capability of regeneration. The present study investigated whether intramyocardial transplantation of human mesenchymal stem cells (hMSCs) or cotransplantation of hMSCs plus human fetal cardiomyocytes (hFCs; 1:1) reconstituted impaired myocardium and improved cardiac function in MI pigs.

Methods And Results: Cultured hMSCs were transfected with green fluorescent protein (GFP).

Overexpression of Na+/Ca2+ exchanger gene attenuates postinfarction myocardial dysfunction.

We monitored myocardial function in postinfarcted wild-type (WT) and transgenic (TG) mouse hearts with overexpression of the cardiac Na(+)/Ca(2+) exchanger. Five weeks after infarction, cardiac function was better maintained in TG than WT mice [left ventricular (LV) systolic pressure: WT, 41 +/- 2; TG, 58 +/- 3 mmHg; P < 0.05; maximum rising rate of LV pressure (+dP/dt(max)): WT, 3,750 +/- 346; TG, 5,075 +/- 334 mmHg/s; P < 0.