DNA-dependent activator of interferon-regulatory factors inhibits hepatitis B virus replication.

Aim: To investigate whether DNA-dependent activator of interferon-regulatory factors (DAI) inhibits hepatitis B virus (HBV) replication and what the mechanism is.

Methods: After the human hepatoma cell line Huh7 was cotransfected with DAI and HBV expressing plasmid, viral protein (HBV surface antigen and HBV e antigen) secretion was detected by enzyme-linked immunosorbent assay, and HBV RNA was analyzed by real-time polymerase chain reaction and Northern blotting, and viral DNA replicative intermediates were examined by Southern blotting. Interferon regulatory factor 3 (IRF3) phosphorylation and nuclear translocation were analyzed via Western blotting and immunofluorescence staining respectively.

Hyper-activated IRF-1 and STAT1 contribute to enhanced interferon stimulated gene (ISG) expression by interferon alpha and gamma co-treatment in human hepatoma cells.

Previous reports suggest that type I and type II Interferon can co-operatively inhibit some virus replication, e.g. HCV, SARS-CoV, HSV-1.

[Establishing DNA chip technique for detecting hepatitis C virus genotypes and primary application].

Objective: To establish a new method based DNA chip technique for detecting HCV genotypes.

Methods: Genotyping probes were designed according to the sequence of HCV 5' NCR to generate DNA chip. The probes on DNA chip contains 5 major genotypes and 8 subtypes.

Gene expression profiles in an hepatitis B virus transfected hepatoblastoma cell line and differentially regulated gene expression by interferon-alpha.

Aim: To study interactions between hepatitis B virus (HBV) and interferon-alpha in liver- derived cells.

Methods: mRNAs were separately isolated from an HBV-transfected cell line (HepG(2)2.2.

Analysis of gene expression in hepatitis B virus transfected cell line induced by interferon.

Infection of hepatitis B virus (HBV) continues to be a significant health problem. alpha interferon (IFN-alpha) and gamma interferon (IFN-gamma) have been proven to be effective in inhibiting HBV replication. To study the global effect of HBV persistent existence on IFN induced cellular gene expression, cDNA microarrays dotted with 14 112 human genes were used to examine the transcriptional changes between an HBV DNA transfected cell line (HepG2.