Biomagnification of polybrominated diphenyl ethers and alternative halogenated flame retardants through food chains of yellowfin tuna (Thunnus albacares) and their prey from the South China Sea and the associated potential health risk.

Polybrominated diphenyl ethers (PBDEs) and their alternative halogenated flame retardants (AHFRs) have gained global attention due to their ubiquitous occurrence, bioaccumulation, and toxic properties. However, the biomagnification of halogenated flame retardants (HFRs), particularly AHFRs, in various food chains is not yet well understood. In this study, yellowfin tuna (Thunnus albacares), along with its prey, flying squid (Sthenoteuthis oualaniensis) and round scad (Decapterus maruadsi), were sampled from the South China Sea (SCS) to investigate the biomagnification potential of PBDEs and AHFRs, including dechlorane plus (DP) and decabromodiphenyl ethane (DBDPE).

Regioselective Multisite Atomic-Chlorine Passivation Enables Efficient and Stable Perovskite Solar Cells.

Passivating defects using organic halide salts, especially chlorides, is an effective method to improve power conversion efficiencies (PCEs) of perovskite solar cells (PSCs) arising from the stronger Pb-Cl bonding than Pb-I and Pb-Br bonding. However, Cl anions with a small radius are prone to incorporation into the perovskite lattice that distorts the lead halide octahedron, degrading the photovoltaic performance. Here, we substitute atomic-Cl-containing organic molecules for widely used ionic-Cl salts, which not only retain the efficient passivation by Cl but also prevent the incorporation of Cl into the bulk lattice, benefiting from the strong covalent bonding between Cl atoms and organic frameworks.

[Microplastics in the stomach and intestine of pelagic squid: A case study of in open sea of Peru].

Microplastic (MP) ingestion by marine animals has been well documented, but less being known about pelagic squid. Jumbo squid supports the world's largest cephalopod fishery and plays an important ecological role in the Eastern Pacific Ocean. In this study, taken from the open sea of the Peruvian Exclusive Economic Zone were selected as research objects.

Knockdown of YKL-40 inhibits angiogenesis through regulation of VEGF/VEGFR2 and ERK1/2 signaling in endometrial cancer.

Studies have demonstrated that small interfering RNA (siRNA) targeting YKL-40 (siYKL-40) inhibits the proliferation, migration, invasion, and induces antiapoptotic abilities of endometrial cancer (EC) HEC-1A cells. However, its effect on angiogenesis is unclear. The present study aimed to investigate the role of YKL-40 in endometrial cancer and the related molecular mechanisms.

Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway.

Cancer-associated fibroblasts cells (CAFs) confer a rapid growth and metastasis ability of endometrial cancer (EC) via exosomes-mediated cellular communication. Long non-coding RNA nuclear enriched abundant transcript 1 (lncRNA NEAT1) drives the malignant phenotypes of EC cells. However, the role of exosomal NEAT1 from CAFs in EC progression remains ambiguous, which needs to be investigated.

-BuOK-catalysed alkylation of fluorene with alcohols: a highly green route to 9-monoalkylfluorene derivatives.

A simple, mild and efficient protocol was developed for the alkylation of fluorene with alcohols in the presence of -BuOK as catalyst, affording the desired 9-monoalkylfluorenes with near quantitative yields in most cases.

Effects of a Small Interfering RNA Targeting YKL-40 Gene on the Proliferation and Invasion of Endometrial Cancer HEC-1A Cells.

Objective: The purpose of this study was to explore the effects of a small interfering RNA (siRNA) targeting YKL-40 on the proliferation and invasion of endometrial cancer (EC) HEC-1A cells.

Methods: We used an siRNA targeting a sequence in YKL-40 (si-YKL-40) to transfect HEC-1A cells. Quantitative real-time polymerase chain reaction assay was performed to investigate the mRNA levels of YKL-40.

The diagnostic and prognostic value of serum YKL-40 in endometrial cancer.

Purpose: To evaluate the diagnostic and prognostic value of serum YKL-40 in endometrial cancer (EC).

Methods: Serum YKL-40 levels were detected and compared in 34 of the 50 cases with EC before surgery, in 22 of the 34 with EC after surgery, in 30 cases with uterine myoma, and in 30 healthy women as normal controls. Receiver operating characteristics (ROC) curves were adopted for diagnosis and calculation of area under each ROC curve in EC.

Expression of HLA-I, CD8, and CD4 and Their Clinical Significance in Cervical Cancer.

Background: The local tissue immune status may play a role in the progression of cervical cancer. The aim of our study is to examine the expression of HLA-I, CD8 and CD4 in various cervical diseases and investigate their association with cervical cancer.

Methods: We chose the tissues of cervical cancer, cervical intraepithelial neoplasia (CIN), chronic cervicitis and peri-cancer tissues, and then detected the expression of HLA-I, CD8 and CD4 using SP immunohistochemistry.

[Prokaryotic expression and purification of human papillomavirus type 11 L2E7 fusion protein vaccine and its immunnogenicity].

Objective: To construct the Escherichia coli (E. coli) prokaryotic expression system pET9aHPV11L2E7, purify the fusion protein L2E7 and study the immunnogenicity of the protein.

Methods: The HPV11 L2, E7 coding region was amplified from condyloma acuminata tissue specimen by PCR.

[Construction and identification of non-replication recombinant vaccinia virus co-expressing human papillomavirus type 16 L1/L2/E6/E7 proteins].

Objective: To generate a human papillomavirus (HPV16) prophylactic and therapeutic vaccine candidate for cervical cancer.

Methods: HPV16 major capsid protein L1 gene/minor capsid protein L2 gene and HPV16 early E6/E7 genes were inserted into a vaccinia virus expression vector. A strain of non-recombinant vaccinia virus containing the sequences was obtained through a homologous recombination and identified.

[Construction of recombinant vaccina virus of HPV16 and its antitumor effect].

Background & Objective: Cervical cancer is tightly related with high-risk types of human papillomavirus (HPV), among which HPV16 is most common. This study was to construct HPV16 L1, L2, E67 coexpression non-replication vaccina virus, and explore its immunization effects.

Methods: HPV16 major capsid protein L1/L2 genes and HPV16 early E6/E7 genes were inserted into a vaccina virus expression vector to construct recombinant vaccina virus NTVJE67CKL1L2 by homologous recombination; the recombinant virus was identified by DNA hybridization and Western blot.

[Value of serum progesterone for ectopic pregnancy in therapeutic effect monitoring].

Objective: To study the value of serum progesterone in selecting the candidate patients with ectopic pregnancy (EP) for methotrexate (MTX) treatment and in monitoring the effect of the treatment.

Methods: Thirty-seven EP patients who were given single-dose intramuscular injection with 50 mg/m2 MTX were divided into success and failure groups according to the effect of the treatment. The serum progesterone concentrations in these patients were measured and compared between the 2 groups, and the receiver-operator curves (ROC) were used to determine the critical serum progesterone levels for assessing the effect of MTX treatment.