Potential of Klotho as a Biomarker for Overwork: A Study of Frontline Medical Workers.

Objective: This study evaluates the utility of serum s-αKlotho levels as a quantifiable biomarker for overwork.

Methods: Frontline medical workers aged 20-55 from Yiling People's Hospital of Yichang were recruited. Criteria included non-smokers, non-heavy drinkers, no chronic medication use, and no acute illnesses recently.

[Effect of Auricularia auricula polysaccharide on PBMCs proliferation and cytokine expression in vitro].

Objective: To investigate the proliferation and cytokine expression of Auricularia auricula polysaccharide (AAP) on peripheral blood monnuclear cells (PBMCs) in vitro.

Methods: AAP was obtained by water extraction and alcohol precipitation, and purified by DEAE-cellulose chromatogram. PBMCs were treated with AAP at different levels.