Publications by authors named "Jiang feng Fan"

Article Synopsis
  • The study investigates how follicle-stimulating hormone (FSH), luteinizing hormone (LH), and miR-23a affect apoptosis and autophagy in yak granulosa cells (GCs), which are crucial for follicular development.
  • Results indicate that overexpressing miR-23a boosts autophagy markers and hormone receptors while decreasing apoptosis indicators.
  • FSH and LH treatments upregulate miR-23a, influencing its role in managing apoptosis, autophagy, and steroid hormone concentrations in yak GCs, potentially helping improve reproductive rates in yaks.
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Placental function is complex and influenced by various factors; furthermore, it depends on a delicate balance between cell proliferation, cell differentiation, and cell death. Bcl-2 and Bax proteins are key apoptosis regulators and are considered to play an important role in the maintenance of both dynamic balance and integrity of many tissues. Changes in Bcl-2 and Bax expressions have been described during different developmental stages in normal human placentas.

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The culture of primary Sertoli cells has become an important resource in the study of their function. However, their use is limited because of contamination of isolated cells with other testicular cells, mainly germ cells. The aim was to establish technique to obtain pure yak Sertoli cells as well as to study the growth kinetics and biological characteristics of Sertoli cells in vitro.

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To clarify the status and distribution of Fas and Fas-Ligand (FasL) in yak's placentomes, immunohistochemistry (IHC) was carried out to analyze the expression and location of Fas and FasL in paraffin embedded sections. The area of positive stained sites was selected and measured using image analyses software (Image Pro-Plus 6.0).

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The present study was carried out to investigate the efficiency of superovulation, oestrus synchronization, and embryo recovery in Tianzhu white yaks and also to confirm the pregnancy rate of black yaks, to which embryos collected from white yaks were transplanted. Forty-seven yaks were selected from different experiment groups, including 10 Tianzhu white female yaks (donor, group A) and 37 black female yaks (recipient, groups B and C). Superovulation of the donor was induced by the application procedure of CIDR-B+FSH+PG.

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