High-dose estrogen impairs demethylation of H3K27me3 by decreasing Kdm6b expression during ovarian hyperstimulation in mice.

Given that ovarian stimulation is vital for assisted reproductive technology (ART) and results in elevated serum estrogen levels, exploring the impact of elevated estrogen exposure on oocytes and embryos is necessary. We investigated the effects of various ovarian stimulation treatments on oocyte and embryo morphology and gene expression using a mouse model and estrogen-treated mouse embryonic stem cells (mESCs). Female C57BL/6J mice were subjected to two types of conventional ovarian stimulation and ovarian hyperstimulation; mice treated with only normal saline served as controls.

Optical Spectroscopic Probing and Atomic Visualization of the Motion of N-Heterocyclic Carbenes on Ag(111).

The application of N-heterocyclic carbenes (NHCs) as versatile anchors for planar surface modifications has been well documented over the past decade. Despite its fundamental importance to the formation of self-assembled NHC monolayers on surfaces, the microscopic mechanism behind the mobility of NHCs has primarily been explored through theoretical studies; an atomic-level experimental understanding of NHC motion on surfaces remains elusive. Here, we combine tip-enhanced Raman spectroscopy (TERS) and scanning tunneling microscopy (STM) to investigate the mobility of a model NHC on Ag(111).

Melatonin attenuates inflammatory bone loss by alleviating mitophagy and lactate production.

Mitochondrial homeostasis plays a major role in the progression of chronic inflammatory bone loss which has a complex pathogenesis with unsatisfactory therapeutic efficiency. Recently, melatonin has been shown to recipient mitochondrial function and bone formation. However, the effects and underlying molecular mechanism of melatonin in chronic inflammatory bone loss remain unclear.

Multimodal discovery of bavachinin A: A natural FLT3 agonist for treating thrombocytopenia.

Background: Radiation-induced thrombocytopenia (RIT) poses a serious risk to patients with cancer undergoing radiotherapy and leads to hemorrhage and mortality. Unfortunately, effective treatment options for RIT are currently limited.

Purpose: This study aimed to discover active compound from Fructus Psoraleae, a traditional Chinese medicine recognized for its hemostatic properties, and to elucidate its mechanism of action in the treatment of RIT.

TCM-KLLaMA: Intelligent generation model for Traditional Chinese Medicine Prescriptions based on knowledge graph and large language model.

Traditional Chinese medicine (TCM) prescriptions are a basic component of TCM treatment, developed by assessing patient symptoms and prescribing a mix of herbs. Accurate prescription generation is critical for enhancing treatment outcomes and maintaining patient safety. However, conventional methods based on Large Language Models (LLMs) focus mainly on symptom information, neglecting other TCM diagnostic expertise, such as tongue and pulse diagnosis, and are prone to hallucination, which is unacceptable in medical applications.

Role of the admission muscle injury indicators in early coagulopathy, inflammation and acute kidney injury in patients with severe multiple injuries.

Backgrounds: Coagulopathy, inflammation and organ failure are common complications in trauma patients. This study aimed to explore the possible role of muscle injury indicators in early coagulopathy, systemic inflammatory response syndrome (SIRS), and acute kidney injury (AKI) in patients with severe multiple trauma.

Methods: A retrospective analysis was performed using trauma center patient data from 2020 to 2023.

A Randomized, Double-Blind, Placebo-Controlled, Multi-centered study to Evaluate the Efficacy and Safety of MEI005 in Reducing Submental Fat in Chinese Adults.

Background: Submental fat (SMF) is a common aesthetic concern traditionally treated with liposuction. Non-surgical alternatives like injectable deoxycholic acid are gaining popularity. However, no related products have been clinically approved in China.

Mechanism of Eustachian Tube Balloon Dilation in Minipigs.

Objective: In this study, in situ balloon dilation of the Eustachian tube (BDET) was performed using minipigs as an animal model to observe the dynamic process of damage and repair of the Eustachian tube mucosa and surrounding tissues.

Methods: The Eustachian tubes of 30 minipigs were divided into seven groups. The five groups included an immediate postoperative group, a 1-week group, a 2-week group, a 3-week group, and a 4-week group following 3 mm balloon dilation.

Global, Regional, and National Burden of Subarachnoid Hemorrhage: Trends From 1990 to 2021 and 20-Year Forecasts.

Background: Subarachnoid hemorrhage (SAH) is a critical condition that has far-reaching implications for public health systems globally due to its severe consequences and long-term disabilities. This study aims to provide a comprehensive analysis of SAH trends from 1990 to 2021 and project future trends up to 2041, aiding in better understanding and management of its global burden.

Methods: We utilized data from the GBD (Global Burden of Disease) 2021 database, using joinpoint regression, frontier, and decomposition analyses to assess changes in SAH burden.

Structural and Functional Insights into GGCX-FIX Interaction: Implications for Vitamin K-Dependent Bleeding Disorders.

Gamma-carboxylation, catalyzed by γ-glutamyl carboxylase (GGCX), is a critical post-translational modification essential for the biological activity of vitamin K-dependent proteins (VKDPs). Mutations in GGCX, depending on their specific location, result in vitamin K-dependent coagulation factor deficiency type 1 (VKCFD1), which encompasses a broad spectrum of clinical manifestations ranging from mild to severe, including bleeding disorders, osteoporosis, and vascular calcification. The limited knowledge of GGCX's structure and functional regions hinders our understanding of the consequences of GGCX mutations and the treatment for VKCFD1.

The research progress on meningeal metastasis in solid tumors.

Meningeal metastasis (MM), particularly Leptomeningeal metastases (LM), represents the advanced stage of solid tumors and poses a significant threat to patients' lives. Moreover, it imposes a substantial burden on society. LM represents the ultimate and most fatal stage of solid tumors, inflicting devastating consequences on patients and imposing a substantial burden on society.

Ruthenium-catalyzed monodeuterium-methylenation of two indole units with vinylene carbonate as a novel methylene precursor.

A new example of deuteration, involving ruthenium-catalyzed monodeuterium-methylenation of indole units with vinylene carbonate, is described. This reaction was used to build an array of promising highly deuterated bisindolylmethanes (d-BIMs). This methodology featured excellent functional group compatibility with diverse substrate categories such as indoles, ,-dialkylanilines, and 1,3-dicarbonyls as well as having showcased the distinct chemical function of VC as an innovative methylene precursor.

Platelet membrane-camouflaged PLGA loaded natamycin improve the prognosis of fungal keratitis.

Fungal keratitis (FK) is a severe infectious corneal disease and a common cause of blindness. At present, natamycin (NATA) is the most commonly prescribed drug for fungal keratitis. However, these disadvantages, including poor water solubility, poor stability, and significant corneal irritation, limit its effect in clinical application.

Microstructural and Micromechanical Properties of Decellularized Fibrocartilaginous Scaffold.

Fibrocartilage decellularized extracellular matrix (dECM) is a promising alternative material for damaged fibrocartilage repair and replacement due to its biomimetic gross morphology and internal microstructure. However, the alterations in the microstructure and micromechanical properties of fibrocartilage after decellularization interfere with the macroscopic functional application of the scaffold. Therefore, this study aims to present an analytical atlas of the microstructure and micromechanics of the fibrocartilaginous dECM scaffold to elucidate the effect of decellularization treatment on the macroscopic function of the scaffold.

An injectable hydrogel for hemostasis and tumor suppression in intraoperative breast cancer.

In the period between surgery and systemic therapy for breast cancer, residual tumor cells may proliferate, leading to tumor recurrence. Additionally, intraoperative wound bleeding may cause surgical failure or the spread of tumor cells. This study introduces an innovative injectable hydrogel composed of oxidized hyaluronic acid (OHA) loaded 5-fluorouracil (5-FU) and N-carboxyethyl chitosan (CEC), designed for intraoperative hemostasis and tumor suppression in intraoperative breast cancer.

Development of potent indole-3-carboxamide autotaxin inhibitors with preferred lipophilicity for in vivo treatment of pulmonary fibrosis.

Autotaxin (ATX), a major source of the lipid mediator lysophosphatidic acid (LPA), plays a critical role in the pathogenesis and progression of pulmonary fibrosis. In this study, with the aim of developing novel ATX inhibitors with preferred lipophilicity, structure-based optimizations of PAT-409 were carried out, leading to the identification of two novel orally active ATX inhibitors, 4 and 29, with IC values of 1.5 nM and 1.

Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats.

Myocardial ischemia‑reperfusion (I/R) injury may lead to dysfunction of signaling pathways related to cell apoptosis, inflammation, oxidative stress, and mitochondrial damage. The present study investigated the defensive effect of liriodendrin, as a natural product isolated from , on reperfusion injury in rats and the underlying mechanisms involved in this process. An rat model of I/R constructed by ligation of the left anterior descending artery, as well as an model using H9C2 cells under hypoxic conditions, was established to assess the cardioprotective effects of liriodendrin.

Case Report: Leaflet thrombosis after transcatheter valve-in-valve aortic valve replacement in prosthetic valve endocarditis.

A 70-year-old female patient with a history of bioprosthetic aortic valve replacement and coronary artery bypass graft presented with bioprosthetic valve failure secondary to prosthetic valve endocarditis. The patient was deemed unsuitable for surgery by the heart team, following which she underwent transcatheter aortic valve-in-valve replacement. This resulted in early death due to myocardial infarction and acute heart failure.

The role of cognitive factors in consumers' perceived value and subscription intention of video streaming platforms.

The video streaming sector has expanded significantly over the past decades despite the competition among service providers generating difficulties in retaining subscribed customers. This study explored the subscription intention by evaluating 35 peer-reviewed journal articles from two popular databases, namely Scopus and Web of Science. Instrumental insights related to the intricate and multidimensional factors that influenced customer decisions to subscribe to or renew video streaming service subscriptions were provided to both academicians and service providers.

Identification of early prediction biomarkers of severity in patients with severe fever with thrombocytopenia syndrome based on plasma proteomics.

Background: Severe fever with thrombocytopenia syndrome (SFTS) is a newly emerging infectious disease. Given its rapid disease progression and high mortality rate, early warning is crucial in improving the outcomes, However, to date, relevant comprehensive predictors or an effective prediction model are still poorly explored.

Methods: A plasma proteomic profile was performed at early stages in patients with SFTS.

Adenosine A receptor-mediated interactions between Th1 T cells and the choroid plexus epithelium via IFN-γ signalling control T-Cell infiltration in experimental autoimmune encephalomyelitis.

Background: Adenosine A receptor (AR) antagonists have been consistently demonstrated to protect against multiple sclerosis (MS) pathology, but AR knockout (AR) mice exhibit exacerbated immune injury, raising concerns regarding the use of AR antagonists for MS treatment. Here, we revealed the critical involvement of AR-mediated interactions between Th1 T cells and the choroid plexus (ChP) epithelium in the pathology of experimental autoimmune encephalomyelitis (EAE).

Methods: We assessed the effects of AR knockout on ChP gateway activity and the interferon gamma (IFN-γ)-secreting capacity of Th1 T cells in an EAE model by immunofluorescence, qPCR and flow cytometry (FCM).

Corrigendum: Research progress of breast cancer surgery during 2010-2024: a bibliometric analysis.

[This corrects the article DOI: 10.3389/fonc.2024.

New Insights into Expanding the Insecticidal Spectrum of dsRNA Mediated by the High Sequence Identity between dsRNA and Nontarget mRNA.

RNA interference (RNAi) is being used to develop methods to control pests, yet its widespread application is limited by the high comprehensive application cost of dsRNAs. Here, we utilized the high identity matching between double-stranded RNA (dsRNA) and nontarget genes to achieve expanding the dsRNA insecticidal spectrum. First, we found that dsRNA was more likely to induce off-target effects in genes with higher transcript levels and higher sequence identity; the existence of either a completely contiguous matching sequence exceeding 15 nt or a partially contiguous matching sequence of 24 nt between genes can lead to off-target effects in .