Genome-wide investigation on the genetic variations of rice disease resistance genes.

Exploitation of plant disease resistance (R) gene in breeding programs has been proven to be the most efficient strategy for coping with the threat of pathogens. An understanding of R-gene variation is the basis for this strategy. Here we report a genome-wide investigation on the variation of NBS-LRR-encoding genes, the common type of R genes, between two sequenced rice genomes, Oryza sativa L.

Energy loss of a charged particle moving over a 2D strongly coupled dusty plasma.

We use molecular dynamics (MD) simulation to evaluate the energy loss of a charged projectile moving parallel to a two-dimensional strongly coupled dusty plasma and compare the results with those obtained from the quasilocalized charge approximation (QLCA) and the Vlasov-random phase approximation. Good agreement is found between the QLCA and MD results when the projectile-dust coupling is weak. In the opposite regime, nonlinear effects in the dust-layer response render the QLCA model increasingly inadequate for calculating the energy losses at low projectile speeds.

Regulation of the EBNA1 Epstein-Barr virus protein by serine phosphorylation and arginine methylation.

The Epstein-Barr virus (EBV) EBNA1 protein is important for the replication and mitotic segregation of EBV genomes in latently infected cells and also activates the transcription of some of the viral latency genes. A Gly-Arg-rich region between amino acids 325 and 376 is required for both the segregation and transcriptional activation functions of EBNA1. Here we show that this region is modified by both arginine methylation and serine phosphorylation.

Excitation of Mach cones and energy dissipation by charged particles moving over two-dimensional strongly coupled dusty plasmas.

We present a theoretical model for studying the interactions of charged particles with two-dimensional strongly coupled dusty plasmas, based on the quasilocalized charge approximation in which the static pair distribution function of a dust layer is determined from a molecular dynamics simulation. General expressions are derived for the perturbed dust-layer density, the induced potential in plasma, and the energy loss of a charged particle moving parallel to the dust layer. Numerical results show that the structure of Mach cones, excited in the dust layer by the charged particle, strongly depends on the plasma parameters such as the coupling parameter, the screening parameter, and the discharge pressure, as well as on the particle speed.

[Extraction of atrazine and its degradation products in soil and determination by high performance liquid chromatography-mass spectrometry].

Atrazine and its degradation products such as deethylatrazine (DEA), deisopropylatrazine (DIA) and hydroxyatrazine (HA) in soil samples were extracted by Waters Oasis MCX cartridges, then determined by high performance liquid chromatography-atmospheric pressure chemical ionization-collision induced dissociation-mass spectrometry (HPLC-APCI-CID-MS). The recoveries of atrazine, DEA, DIA and HA fortified in the range of 4.5-120 ng/g were 40.

Electro-acupuncture preconditioning abrogates the elevation of c-Fos and c-Jun expression in neonatal hypoxic-ischemic rat brains induced by glibenclamide, an ATP-sensitive potassium channel blocker.

This study aimed to clarify the neuroprotective mechanism of electro-acupuncture (EA) preconditioning on hypoxic-ischemic brain injury (HIBI). Using Western blot, the expression of c-fos protein (c-Fos) and c-jun protein (c-Jun) induced by glibenclamide, an ATP-sensitive potassium (K(ATP)) channel blocker was examined from cerebral cortical and hippocampal samples in neonatal hypoxic-ischemic rats, with or without EA preconditioning. EA was performed on Hegu (LI4), a well-known acupoint commonly used in Oriental medicine for the treatment of neuronal injury resulting from hypoxia-ischemia (HI).

[The concentration and source of atrazine residue in water of Guanting reservoir].

The atrazine in water was determined by SPE-LC-MS. It only took 40 min to analysis a sample. The detection limit was 10 ng/L.