[Pathogenic and clinicopathologic study of invative mycosis in nose and paranasal sinus].

Objective: To investigate the mechanism of fungi invasion into the mucosal tissue, and to analyse the clinical-pathological relationship.

Methods: The clinical data of 16 patients with invasive mycosis in the nose and paranasal sinus proved by pathologic examination between Apr, 1984 and Aug, 2002 were reviewed retrospectively. There were 7 males and 9 females with an average age of 48.

[Expression of pulmonary surfactant-associated protein D and its significance in human fetal lung].

Objective: To observe the expression of pulmonary surfactant-associated protein D (SP-D) during human fetal lung development.

Methods: Human fetal lung tissues from 11- to 36-week-old fetuses were assayed for lung maturity with HE staining and SP-D expression by means of immunohistochemistry.

Results: SP-D expression was located in the bronchial epithelium and alveolar epithelial type II cells(AEC II) of the lung tissues since the 12th week, and gradually increased during the canalicular to saccular stages with fetal development, at the point of which SP-D-positivity began a shift from the proximal bronchial epithelium to AEC II , and evidently located in the alveoli from the advanced stage of lung development till postnatal stage.

Time-resolved undersampled projection reconstruction magnetic resonance imaging of the peripheral vessels using multi-echo acquisition.

The hybrid projection reconstruction (PR) imaging provides high temporal resolution through an undersampled PR acquisition for the in-plane dimensions and Cartesian slice encoding for the through-plane dimension. The undersampling of projection data introduces streak artifact, which may severely compromise image quality. This study reports on a combination of multi-echo acquisition with time-resolved undersampled PR imaging and its application to peripheral magnetic resonance angiography.

Floating table isotropic projection (FLIPR) acquisition: a time-resolved 3D method for extended field-of-view MRI during continuous table motion.

In this work, 3D vastly undersampled isotropic projection (VIPR) acquisition is used simultaneously with continuous table motion to extend the superior/inferior (S/I) FOV for MR angiograms. The new technique is termed floating table isotropic PR (FLIPR). The use of 3D PR in conjunction with table motion obviates the need to locate and prescribe imaging volumes containing the major blood vessels over the large superior-inferior (S/I) ranges encountered in whole-body imaging.

Contrast-enhanced peripheral magnetic resonance angiography using time-resolved vastly undersampled isotropic projection reconstruction.

Purpose: To investigate the application of time-resolved vastly undersampled isotropic projection reconstruction (VIPR) in contrast-enhanced magnetic resonance angiography of the distal extremity (single station), and peripheral run-off vasculature in the abdomen, thigh, and calf (three stations).

Materials And Methods: Time-resolved distal extremity imaging was performed using VIPR sequence through the comparison of two acquisition matrix sizes: 256 with TR/TE=3.7/1.

[Effects of neonatal calf serum on differentiation of human fetal neural stem cells in the hippocampus].

Objective: To investigate the effects of neonatal calf serum on the differentiation of human neural stem cells in the hippocampus.

Methods: The effects of various concentrations of neonatal calf serum on the differentiation of human fetal neural stem cells in the hippocampus were observed in cell culture experiment and immunocytochemical staining.

Results: Neonatal calf serum efficiently induced the differentiation of human fetal neural stem cells into neurons and astrocytes, whose amount varied between serum-free cell culture and the culture with neonatal calf serum of different concentrations.

[Rapid diagnosis of common pathogenic bacteria infection in newborn infants by 16SrDNA oligonucleotide array].

Objective: The rapid identification of pathogenic bacteria is important for earlier effective patient management and antimicrobial therapy, especially for the infant patient, whose immunological system is not fully developed. However conventional microbiogical techniques of bacterial identification, culture and isolation of pathogenic bacteria, identification by biochemistry and serological assay, are time-consuming and require intensive labor. On the basis of special gene sequence, PCR provides simple and rapid way to identify bacteria.

[Repair of the rabbit ear cartilage defects with transforming growth factor-beta1 and allogenic chondrocyte/poly-DL-lactide higher porosity polymer].

Objective: To study the repair of the rabbit ear cartilage defects with transforming growth factor-beta1 (TGF-beta1) and allogenic chondrocyte/poly-DL-lactide (PDLLA) higher porosity polymer.

Methods: A total of 18 rabbits were divided into the TGF-beta1 and chondrocytes/PDLLA graft group, the chondrocytes/PDLLA graft group, and the blank control group. Samples were taken out at 4, 12 and 18 weeks after implantation.

[Expression of telomerase reverse transcriptase and its significance in human fetal lung development].

Objective: To investigate the expression and distribution of telomerase reverse transcriptase (hTERT) and its significance during the development of epithelial stem cells in human fatal lungs.

Methods: Human lung tissues were obtained at abortion from 37 fetuses 10-34 weeks of gestational age with parental consent, and the expression of hTERT in these tissues was examined by immunohistochemistry.

Results: In fetuses of 10 weeks old, hTERT was detected mainly in the epithelial cells of the proximal bronchi in the fetal lung, and migrated gradually to the distal bronchi as the lung developed.

Noise reduction in MR angiography with nonlinear anisotropic filtering.

Purpose: To evaluate three-dimensional nonlinear anisotropic filtering in suppressing image noise in high spatial resolution magnetic resonance angiograms (MRA) acquired with hybrid undersampled projection reconstruction and phase contrast vastly undersampled isotropic projection reconstruction (PC-VIPR).

Materials And Methods: Three-dimensional nonlinear anisotropic filtering was quantitatively analyzed and evaluated through the measurement of contrast to noise ratio (CNR) in PC-VIPR images and contrast enhanced peripheral MRA images. To filter MRA images with ultra-high spatial resolution and poor CNR, a spatial frequency dependent nonlinear anisotropic filtering algorithm was proposed that uses two-step processing to filter the whole spatial frequency data.

[Surgical treatment of primary tracheal adenoid cystic carcinoma: report of 11 cases].

Objective: To evaluate the clinical characteristics, diagnosis and treatment of primary tracheal adenoid cystic carcinoma.

Methods: The data of 11 patients with primary tracheal adenoid cystic carcinoma were reviewed. The age of the patients ranged from 30 to 49 years.

[Preparation and identification of monoclonal antibodies against human pulmonary surfactant-associated protein A].

Objective: To prepare monoclonal antibody (mAb) against human pulmonary surfactant-associated protein A (SP-A) and evaluate its specificities.

Methods: The hybridoma cell line secreting SP-A mAb was established to induce the production of SP-A ascites fluid in BALB/c mice immunized with SP-A. The ascites fluid was then collected and purified through (NH4)2SO4 salting-out procedure, and the titer and specificity of the purified monoclonal anti-SP-A antibodies were determined by Western blotting and indirect enzyme-linked immunosorbent assay, respectively.

[Purification of porcine pulmonary surfactant by CO2 supercritical fluid extraction].

Objective: To develop a supercritical fluid extraction (SFE) method for purifying porcine pulmonary surfactant.

Method: An orthogonal design with 4 factors and 4 levels was used to optimize the SFE conditions.

Results: The best extraction conditions were established as the following: pressure 30 MPa, temperature 50 degrees Celsius, dynamic extraction for 4 h with 0.

Antiviral flavonoids from the root bark of Morus alba L.

A prenylated flavonoid, moralbanone, along with seven known compounds kuwanon S, mulberroside C, cyclomorusin, eudraflavone B hydroperoxide, oxydihydromorusin, leachianone G and alpha-acetyl-amyrin were isolated from the root bark of Morus alba L. Leachianone G showed potent antiviral activity (IC(50) = 1.6 microg/ml), whereas mulberroside C showed weak activity (IC(50) = 75.

[Effects of Allogeneic Transplantation of T Helper-2 Cells on Graft-versus-Host Disease and Graft-versus-Leukemia Effect].

This study was undertaken to explore whether the graft-versus-host-disease could be decreased and graft-versus-leukemia effect be retained by transplantation of allogeneic T helper-2 (Th2) cells. T cells from C57BL/6(H-2b) mice were incubated and polarized with rmIL-4, Con A and ionomycin in vitro, and then, the T cells were mixed with marrow cells and transplanted into recipient BALB/c(H-2d) mice bearing erythroleukemia cells. The occurence of GVHD and GVL effect was observed.

Isolation and purification of pulmonary surfactant-associated protein A from human amniotic fluid and its bioactivity assessment.

OBJECTIVE: To isolate and purify pulmonary surfactant-associated protein A(SP-A) from human amniotic fluid. METHODS: Human amniotic fluid was collected from 11 parturients and SP-A was isolated by extraction and dialysis followed by purification procedures. The specificity of SP-A was detected by Western blotting and the bioactivity assessed by MTP-1 film balance.

Saccopetrins A and B, two novel gamma-lactones from Saccopetalum prolificum.

Two new gamma-lactones, saccopetrin A (1) and saccopetrin B (2), together with 10 known compounds have been isolated from the roots of Saccopetalum prolificum. Their structures were established by spectroscopic and chemical methods. The absolute configuration of 1 was determined by chemical transformation and the Mosher method.

Combined time-resolved and high-spatial-resolution 3D MRA using an extended adaptive acquisition.

Purpose: To combine the benefits of time-resolved dynamic imaging and single elliptical centric acquisitions in a reasonable scan time.

Materials And Methods: A time series of images with moderate spatial resolution was acquired using the 3D Time-Resolved Imaging of Contrast KineticS (3D TRICKS) technique with elliptical centric encoding during contrast arrival. Following venous opacification, a complete large centrically encoded k-space volume was acquired.

Antiviral Chinese medicinal herbs against respiratory syncytial virus.

Forty-four medicinal herbs were tested for antiviral activities against respiratory syncytial virus (RSV) by means of the cytopathologic effect (CPE) assay. Twenty-seven of the 44 medicinal herbs showed potent or moderate antiviral activities against RSV with 50% inhibition concentration (IC(50)) ranging from 6.3 to 52.