A novel designed anti-PD-L1/OX40 bispecific antibody augments both peripheral and tumor-associated immune responses for boosting anti-tumor immunity.

Bispecific antibodies (BsAbs) combining simultaneous PD-L1 blockade and conditional co-stimulatory receptor activation have been developed to improve immune checkpoint therapy response. However, several PD-L1-based BsAbs have encountered clinical challenges, including insufficient activity or unexpected toxicity. In this study, we propose OX40 as a more suitable target partner for PD-L1-based BsAb design compared to ongoing clinical partners (CD27 and 4-1BB).

A GMP-compliant manufacturing method for Wharton's jelly-derived mesenchymal stromal cells.

Background: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) hold great therapeutic potential in regenerative medicine. Therefore, it is crucial to establish a Good Manufacturing Practice (GMP)-compliant methodology for the isolation and culture of WJ-MSCs. Through comprehensive research, encompassing laboratory-scale experiments to pilot-scale studies, we aimed to develop standardized protocols ensuring the high yield and quality of WJ-MSCs manufacturing.

Peanut oil odor enhances the immunomodulatory effect on immunosuppressed mice by regulating the cAMP signaling pathway the brain-spleen axis.

The role of sniffing relative to immune function has attracted considerable attention. The present study investigated the immunomodulatory effects of peanut oil odor on cyclophosphamide (CTX)-induced immunosuppressed mice. The subset of mice subjected to prolonged (8 h) sniffing peanut oil odor (PL) demonstrated significantly elevated levels of agouti-related peptide, neuropeptide Y, and glutamate ( < 0.

Inhibition of bleomycin-induced pulmonary fibrosis in SD rats by sea cucumber peptides.

Background: Pulmonary fibrosis (PF) is the terminal manifestation of a type of pulmonary disease, which seriously affects the respiratory function of the body, and with no effective cure for treatment. This study evaluated the effect of sea cucumber peptides (SCP) on bleomycin-induced SD rat PF.

Results: SCP can inhibit the PF induced by bleomycin.

[Characteristics of Cadmium Concentration and Transport of in Farmland with Different Cadmium Pollution Degrees].

This study aimed to elucidate the cadmium (Cd) concentration and transport characteristics of in farmland with different Cd pollution degrees, so as to provide a reference basis for phytoremediation of Cd-contaminated farmland. The multi-point experiments in farmland with different Cd pollution degrees[(Cd) 0.32-38.

The ability of twisted nanographene for removal of Pb, Hg and Cd ions from wastewater: Computational study.

Context: Heavy metal ion removal from wastewater has become a global concern due to its extensive negative effects on human health and the environment. The density functional theory is employed to investigate the possibility of removing Pb, Hg, and Cd ions from wastewater using nano-graphene. Researchers have shown that NG can efficiently remove heavy metals from media.

Identification and sensing of hydrogen fluoride (HF) on aluminum phosphide (AlP) nanocage in both gas and water phases: electronic study via density-functional theory computations.

Context: Hydrogen fluoride (HF) is extensively present in environmental and industrial pollutants. It may harm the health of humans and animals. This work evaluated the adsorption of an (HF)n linear chain (n = 1, 2, 3, and 4) onto an AlP nanocage through ab initio calculations for the evaluation of its performance in sensing and monitoring (HF)n within aqueous and gaseous media.

Structural Characteristics and Immunomodulatory Effects of Melanoidins from Black Garlic.

Melanoidins are considered to have several biological activities. In this study, black garlic melanoidins (MLDs) were collected using ethanol solution extraction; 0%, 20%, and 40% ethanol solutions were used for chromatography. Three kinds of melanoidins were produced by macroporous resin, named MLD-0, MLD-20, and MLD-40.

Effect of biochar with different particle sizes on the sorption-desorption characteristics of soil phosphorus.

The size of particles determines the adsorption reaction. In this study, three different particle sizes of biochar (0.25-1 mm, 0.

Black garlic melanoidins inhibit in vitro α-amylase and α-glucosidase activity: the role of high-molecular-weight fluorescent compounds.

Background: Black garlic (Allium sativum L.) melanoidins (MLDs) are produced by Maillard reaction under high temperature and high humidity, and has a variety of biological activities. The aim of this study was to analyze the structural characteristics and investigate α-amylase and α-glucosidase in vitro inhibitory activity of black garlic MLDs.

Fabrication and Characterization of Konjac Glucomannan/Oat β-Glucan Composite Hydrogel: Microstructure, Physicochemical Properties and Gelation Mechanism Studies.

The aim of this study was to evaluate the effect of oat β-glucan on the formation mechanism, microstructure and physicochemical properties of konjac glucomannan (KGM) composite hydrogel. The dynamic rheology results suggested that the addition of oat β-glucan increased the viscoelastic modulus of the composite hydrogel, which was conducive to the formation of a stronger gel network. Gelling force experiments showed that hydrogen bonds and hydrophobic interactions participated in the formation of the gel network.

Association between the platelet-lymphocyte ratio and short-term mortality in patients with non-ST-segment elevation myocardial infarction.

Background: Previous studies have shown that inflammation plays an important role in atherosclerosis and cardiovascular disease. Platelet to lymphocyte ratio (PLR) has been reported as a novel inflammatory marker. However, it is not clear whether PLR is associated with short-term all-cause mortality in critically ill patients with non-ST-segment elevation myocardial infarction (NSTEMI).

Different Molecular Weight Black Garlic Melanoidins Alleviate High Fat Diet Induced Circadian Intestinal Microbes Dysbiosis.

The purpose of this study is to explore the effects of different molecular weight black garlic melanoidins (MLDs) on high fat diet (HFD) induced dysrhythmia of intestinal microorganisms. The results showed that a HFD disturbed the periodic fluctuation of the gut microbiome and that oral gavage of low molecular weight melanoidin (LMM) or high molecular weight melanoidin (HMM) reversed these cyclical variations in part, which resulted in an increase in the number of bacteria producing short-chain fatty acids (SCFAs) and a decrease in the oscillation of inflammation-related bacteria within a specific time period over the course of 1 day. Moreover, structural analysis showed different structure characterizations of LMM and HMM, which are related to the differences in flora oscillation.

Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.

The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs). The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses. The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.

Evaluation on the physicochemical and digestive properties of melanoidin from black garlic and their antioxidant activities in vitro.

As the important compounds in black garlic, the physicochemical properties and bioactivities of melanoidin (MLD) were investigated in this study. The results showed that MLD possessed strong metal-ion chelating capacity and radical scavenging activities which were positively correlative with molecular weight (MW). During the simulated digestion in vitro, the ultraviolet absorption, browning degree and MW distribution of MLD remained the same as initial.

Co-Amendment of S and Si Alleviates Cu Toxicity in Rice ( L.) Grown on Cu-Contaminated Paddy Soil.

With irrigation using waste water, application of sewage sludge, and development of mine exploration, copper (Cu) contamination in some paddy fields has become increasingly serious. A greenhouse pot experiment was conducted using a factorial design with three sulfur (S) application rates (i.e.

Inhibition of Neddylation Modification Sensitizes Pancreatic Cancer Cells to Gemcitabine.

Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer death in the USA with a 5-year survival rate less than 3% to 5%. Gemcitabine remains as a standard care for PDAC patients. Although protein neddylation is abnormally activated in many human cancers, whether neddylation dysregulation is involved in PDAC and whether targeting neddylation would sensitize pancreatic cancer cells to gemcitabine remain elusive.

NEDD8-activating enzyme inhibitor, MLN4924 (Pevonedistat) induces NOXA-dependent apoptosis through up-regulation of ATF-4.

It has been reported that MLN4924 can inhibit cell growth and metastasis in various kinds of cancer. We have reported that MLN4924 is able to inhibit angiogenesis through the induction of cell apoptosis both in vitro and in vivo models. Moreover, Neddylation inhibition using MLN4924 triggered the accumulation of pro-apoptotic protein NOXA in Human umbilical vein endothelial cells (HUVECs).

Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking.

Dopamine (DA) homeostasis is essential for a variety of brain activities. Dopamine transporter (DAT)-mediated DA reuptake is one of the most critical mechanisms for normal DA homeostasis. However, the molecular mechanisms underlying the regulation of DAT activity in the brain remain poorly understood.

Design of a dual-mode electrochemical measurement and analysis system.

A dual-mode electrochemical measurement and analysis system is proposed. This system includes a dual-mode chip, which was designed and fabricated by using TSMC 0.35 µm 3.

[Effects of rice straw returning mode on rice grain yield and soil carbon pool management index in double rice-cropping system].

A 2-year field experiment was conducted to study the effects of different rice straw returning modes on the rice grain yield and soil carbon pool management index (CPMI) in a double rice-cropping system. Four treatments were installed, including balanced mineral fertilization (NPK), NPK plus cut rice straw returning (SNPK), NPK plus incinerated rice straw returning (SINPK), and no fertilization (CK). In treatments SNPK and SINPK, the 2 years average grain yield of early rice and late rice was basically the same, and much higher than that in treatment NPK, with an increment of 5.

Interleukin-22 protects rat PC12 pheochromocytoma cells from serum deprivation-induced cell death.

Interleukin-22 (IL-22), an IL-10 family cytokine, mediates the crosstalk between leukocytes and epithelial cells. Previous studies reported that IL-22 expresses in mouse brain, and the rat PC12 cells are responsive to IL-22 stimulation. However, the biological roles of IL-22 in neuronal cells remain largely unknown.